LOCUS       sequence-388353-         404 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of P53 CTD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Krois AS, Park S, Martinez-Yamout MA, Dyson HJ, Wright PE
  TITLE     Mapping Interactions of the Intrinsically Disordered C-Terminal 
            Regions of Tetrameric p53 by Segmental Isotope Labeling and NMR.
  JOURNAL   Biochemistry. 2022 Dec 6;61(23):2709-2719. doi: 
            10.1021/acs.biochem.2c00528. Epub 2022 Nov 15.
  PUBMED    36380579
REFERENCE   2  (bases 1 to 404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.biochem.2c00528"; journalName: "Biochemistry"; date: 
            "2022-12-6- 6"; volume: "61"; issue: "23"; pages: "2709-2719"
COMMENT     Sequence Label: H6GB1tevP53(363-393)
FEATURES             Location/Qualifiers
     source          1..404
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..238
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             263..283
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gaaataattt tgtttaactt taataaggag atataccatg ggccatcatc atcatcatca
       61 cagcagcggc atgacttaca aactgatcct taacggtaaa actttgaaag gcgaaaccac
      121 taccgaagct gttgatgctg cgactgcaga aaaagttttc aaacagtacg ctaacgacaa
      181 cggtgttgac ggtgaatgga cttacgacga tgcgactaag accttcaccg ttactgaaga
      241 attcagcagc ggcagcagcg gcgaaaacct gtattttcag ggctcccata gggctcactc
      301 cagccacctg aagtccaaaa agggtcagtc tacctcccgc cataaaaaac tcatgttcaa
      361 gacagaaggg cctgactcag actaatagaa gcttgcggcc gcac
//
