LOCUS       sequence-38841-e         610 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Noyes MB, Meng X, Wakabayashi A, Sinha S, Brodsky MH, Wolfe SA
  TITLE     A systematic characterization of factors that regulate Drosophila 
            segmentation via a bacterial one-hybrid system.
  JOURNAL   Nucleic Acids Res. 2008 May;36(8):2547-60. doi: 10.1093/nar/gkn048. 
            Epub 2008 Mar 10.
  PUBMED    18332042
REFERENCE   2  (bases 1 to 610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkn048"; journalName: "Nucleic Acids Res"; date: 
            "2008-05"; volume: "36"; issue: "8"; pages: "2547-60"
COMMENT     Sequence Label: pB1H2w2-12-Oct1
FEATURES             Location/Qualifiers
     source          1..610
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(27..209,210..218,219..386))
                     /codon_start=1
                     /product="composite human DNA-binding domain composed of 
                     domains from the transcription factors Zif268 and Oct-1 
                     (Pomerantz et al., 1995)"
                     /label=ZFHD1
                     /label=ZFHD1 (DNA binding domain)
                     /translation="RPYACPVESCDRRFSRSDELTRHIRIHTGQKPFQCRICMRNFSRS
                     DHLTTHIRTHTGTGRRRKKRTSIETNIRVALEKSFLENQKPTSEEITMIADQLNMEKEV
                     IRVWFCNRRQKEKRIN"
     CDS             complement(426..449)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tctctagagg atccttagct tggtgggttg attctttttt ctttctggcg gcggttacag
       61 aaccaaacac gaatcacctc tttttccata ttgagctgat cagcaatcat agtgatctct
      121 tccgaggtag gcttttgatt ctccaagaaa ctcttctcta aggccacacg gatgttggtc
      181 tctatgctgg tgcgtttctt cctcctacgc ccggtaccgg tgtgggtgcg gatgtgggtg
      241 gtaaggtggt cactacgact gaagttacgc atgcagattc gacactggaa gggcttctgg
      301 cctgtgtgga tgcggatatg gcgggtaagc tcatccgagc gagaaaagcg gcgatcgcag
      361 gactcgacag ggcaagcata tgggcgcgcc gtgccgtgcg gcggggtctt ggagccggtc
      421 cggaacttgt cgtcgtcatc cttgtagtcc gcggccgcac gaccttcagc aatagcggta
      481 acggcttgta attcagcggc ttcctgctct tgctgttcct ggcgttcgcg aacgtcgagg
      541 atctggttgt tgatcagacc ttcttcgatt tcgcgcagcg cgattacagt ggttttatcg
      601 ttttcttccg
//
