LOCUS       sequence-388558-        4848 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A human PTPN22 mutant (intron 18-runon) in Gateway pDONR221.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Ge Y, Onengut-Gumuscu S, Quinlan AR, Mackey AJ, Wright JA, Buckner 
            JH, Habib T, Rich SS, Concannon P
  TITLE     Targeted Deep Sequencing in Multiple-Affected Sibships of European 
            Ancestry Identifies Rare Deleterious Variants in PTPN22 That Confer 
            Risk for Type 1 Diabetes.
  JOURNAL   Diabetes. 2016 Mar;65(3):794-802. doi: 10.2337/db15-0322. Epub 2015 
            Dec 2.
  PUBMED    26631741
REFERENCE   2  (bases 1 to 4848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.2337/db15-0322";
            journalName: "Diabetes"; date: "2016-03"; volume: "65"; issue: "3"; 
            pages: "794-802"
COMMENT     Sequence Label: pDONR221-In18runon-PTPN22
FEATURES             Location/Qualifiers
     source          1..4848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2973..3072)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3089..3108)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3090..3108)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3113..3129)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3113..3129)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3231..3250)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3242..4051
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3309..3328)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4198..4786
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4687..4706
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc atggaccaaa gagaaattct gcagaagttc ctggatgagg cccaaagcaa
      721 gaaaattact aaagaggagt ttgccaatga atttctgaag ctgaaaaggc aatctaccaa
      781 gtacaaggca gacaaaacct atcctacaac tgtggctgag aagcccaaga atatcaagaa
      841 aaacagatat aaggatattt tgccctatga ttatagccgg gtagaactat ccctgataac
      901 ctctgatgag gattccagct acatcaatgc caacttcatt aagggagttt atggacccaa
      961 ggcttatatt gccacccagg gtcctttatc tacaaccctc ctggacttct ggaggatgat
     1021 ttgggaatat agtgtcctta tcattgttat ggcatgcatg gagtatgaaa tgggaaagaa
     1081 aaagtgtgag cgctactggg ctgagccagg agagatgcag ctggaatttg gccctttctc
     1141 tgtatcctgt gaagctgaaa aaaggaaatc tgattatata atcaggactc taaaagttaa
     1201 gttcaatagt gaaactcgaa ctatctacca gtttcattac aagaattggc cagaccatga
     1261 tgtaccttca tctatagacc ctattcttga gctcatctgg gatgtacgtt gttaccaaga
     1321 ggatgacagt gttcccatat gcattcactg cagtgctggc tgtggaagga ctggtgttat
     1381 ttgtgctatt gattatacat ggatgttgct aaaagatggg ataattcctg agaacttcag
     1441 tgttttcagt ttgatccggg aaatgcggac acagaggcct tcattagttc aaacgcagga
     1501 acaatatgaa ctggtctaca atgctgtatt agaactattt aagagacaga tggatgttat
     1561 cagagataaa cattctggaa cagagagtca agcaaagcat tgtattcctg agaaaaatca
     1621 cactctccaa gcagactctt attctcctaa tttaccaaaa agtaccacaa aagcagcaaa
     1681 aatgatgaac caacaaagga caaaaatgga aatcaaagaa tcttcttcct ttgactttag
     1741 gacttctgaa ataagtgcaa aagaagagct agttttgcac cctgctaaat caagcacttc
     1801 ttttgacttt ctggagctaa attacagttt tgacaaaaat gctgacacaa ccatgaaatg
     1861 gcagacaaag gcatttccaa tagttgggga gcctcttcag aagcatcaaa gtttggattt
     1921 gggctctctt ttgtttgagg gatgttctaa ttctaaacct gtaaatgcag caggaagata
     1981 ttttaattca aaggtgccaa taacacggac caaatcaact ccttttgaat tgatacagca
     2041 gagagaaacc aaggaggtgg acagcaagga aaacttttct tatttggaat ctcaaccaca
     2101 tgattcttgt tttgtagaga tgcaggctca aaaagtaatg catgtttctt cagcagaact
     2161 gaattattca ctgccatatg actctaaaca ccaaatacgt aatgcctcta atgtaaagca
     2221 ccatgactct agtgctcttg gtgtatattc ttacatacct ttagtggaaa atccttattt
     2281 ttcatcatgg cctccaagtg gtaccagttc taagatgtct cttgatttac ctgagaagca
     2341 agatggaact gtttttcctt cttctctgtt gccaacatcc tctacatccc tcttctctta
     2401 ttacaattca catgattctt tatcactgaa ttctccaacc aatatttcct cactattgaa
     2461 ccaggagtca gctgtactag caactgctcc aaggatagat gatgaaatcc cccctccact
     2521 tcctgtacgg acacctgaat catttattgt ggttgaggaa gctggagaat tctcaccaaa
     2581 tgttcccaaa tccttatcct cagctgtgaa ggtaaaaatt ggaacatcac tggaatgggg
     2641 tggaacatct gaaccaaaga aatttgatga ctctgtgata cttagaccaa gcaagagtgt
     2701 aaaactccga agtcctaaat cagaactaca tcaagatcgt tcttctcccc cacctcctct
     2761 cccagaaaga actctagagt ccttctttct tgccgatgaa gattgtatgc aggcccaatc
     2821 tatagaaaca tattctacta gctatcctga caccatggaa aattcaacat cttcaaaaca
     2881 gacactgaag actcctggaa aaagtttcac aaggagtaac gtaaagaaga taagagtttt
     2941 gggaaacatg ccggaaactt tcccttactg agacccagct ttcttgtaca aagttggcat
     3001 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
     3061 aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc
     3121 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
     3181 aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt
     3241 tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga
     3301 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     3361 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     3421 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
     3481 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
     3541 cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     3601 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
     3661 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
     3721 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     3781 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     3841 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
     3901 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
     3961 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     4021 gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg
     4081 gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt
     4141 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4201 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4261 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4321 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     4381 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4441 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4501 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4561 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4621 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4681 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4741 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4801 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt
//
