LOCUS       sequence-388691-        8703 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Loads a truncated payload; for identification of Pdp1 packaging 
            domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8703)
  AUTHORS   Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F
  TITLE     Programmable protein delivery with a bacterial contractile injection
            system.
  JOURNAL   Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7.
            Epub 2023 Mar 29.
  PUBMED    36991127
REFERENCE   2  (bases 1 to 8703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-05870-7"; journalName: "Nature"; date: 
            "2023-04"; volume: "616"; issue: "7956"; pages: "357-364"
COMMENT     Sequence Label: pBR322-Pdp1_aa1-190-HiBiT
FEATURES             Location/Qualifiers
     source          1..8703
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(559..1350,1351..1419))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1182..1201
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1420..1524)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1592..1610
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(1637..1642,1643..1659,1660..1665)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             7884..8075
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(8069..8091)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8177..8317
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8232..8251
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8332..8349)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(8503..8703,1..388))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8583..8602)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
       61 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
      121 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
      181 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
      241 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
      301 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
      361 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
      421 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
      481 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
      541 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
      601 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
      661 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
      721 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
      781 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
      841 agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc
      901 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
      961 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     1021 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     1081 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     1141 tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag
     1201 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     1261 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     1321 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     1381 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     1441 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     1501 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     1561 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     1621 tcaagaattc tcatgtttga cagcttatca tcgataagct ttaatgcggt agtttatcac
     1681 agttaaattg ctaacgcagt caggcaccgt gtatgaaatc taacaatgcg ctcatcgtca
     1741 tcctcggcac cgtcaccctg gatgctgtag gcataggctt ggttatgccg gtactgccgg
     1801 gcctcttgcg ggatatcgtc cattccgaca gcatcgccag tcactatggc gtgctgctag
     1861 cgctatatgc gttgatgcaa tttctatgcg cacccgttct cggagcactg tccgaccgct
     1921 ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac tacgcgatca
     1981 tggcgaccac acccgtcctg tggatccatt tttttaatta aataaaggaa ttaatattga
     2041 tttgatagag catgtaatca ataacttcta tgaaaatgtt gaaaaacaat acacggaaac
     2101 acttaactta caattaaatt gttaaaggag atgatgataa gaatggtcag tcaccagata
     2161 aaatgatctc tggtcaattt taggaaagct gaaaaaacct gaccaaatgc gtcgtaaatt
     2221 ctatgcttag aaagactcgg tgccagtgta atacctaaga tatggttttt atttccatat
     2281 ctgttcgaaa acttagaaat aacaagttca acaataacca gggactttaa atatgcctag
     2341 atatgctaat tatcagataa accccaaaca gaatattaaa aattcacatg ggaaatcttc
     2401 atcgtcagat ttttctagtg ggtacctttc attctcaaat aattcgcttg atgacccttt
     2461 tattcggcag caagttaaga gagaatttat ttgggaggga catatgaagg agattgaaga
     2521 agcttcaaga ttaggtaatt ttgctgtcag ttttcgggct gcgggcgggc caacactcag
     2581 agctttgggc aaaggtgcgg cagccaaggg gcatgatatt ttggaaaaaa ccattaaacc
     2641 cggctcaatt aacaaagctt atcccaaaga cgaagcttca gatgtaataa agaaagtaca
     2701 ggaagcgggt attgaaggct atgtaggcca ttgggataaa aaaactggcc gccttttggg
     2761 tatttacatg agtagtggtc acggtttatc agatgaacag gtcaatggaa aaatttatcc
     2821 tatagactta aacaatctgg aagcgagtct ctcagccttg aaagcaaaag agaactgggc
     2881 tgcactacct tttactggtg acgggagctc cggtgtgagc ggctggcggc tgttcaagaa
     2941 gattagctga tatggatttt catgtcatca ggagaatatt caaaattctg tgtcacacaa
     3001 aatattttac agcgtaatca caccattaag aggggcatca aaataaatgg ttaactggga
     3061 caatgttgaa tttcattttt ataaggcatt gtccgtatta tccaccatcc agatcaacgg
     3121 attcagatgg tgtaaagaaa gcatatagct ggtaggtaaa attaagaagt gtagttttat
     3181 aaagtattaa tggagaacga tgaatttaaa gtaaatggag gaaggttagt ttcactatca
     3241 aattacagga ccaaaaatat ggagtattcc atcttctttg gtgaattaag tcttgaagac
     3301 ttcaacaaat attgcctgga actatgggcc tgtgaatggt attggagtat catttataac
     3361 gactatttcg ataggaaatt taaagtcttg ttgaaaatga ctaatcgtct aaaagtatgt
     3421 ctggagaaat aggtttaatc tctatattta acggttttat tttcatctat ttactaatgg
     3481 taaatttttt tactctttga atatattggt gagtttgtta tccccattag ttaccagggt
     3541 attattaatg agtgaaattc aattttattg atattaaagt caaatttaca aatgatgact
     3601 ttattatttt ttatttatta attttaaatt attttttgtt aatctttact ataaatgaat
     3661 tttttgcttg catggacttg atttttttat atataacatt gacaaatatt aattcatata
     3721 tatgattgtt atagtgagtt atttaattga ttcgactaac aatttcattg taatttttca
     3781 agatagcagt caattgctat aacaagcatc tttctcgtca aaggaaaatg tgtatggcct
     3841 ataagcagca acttgaaaga tgaaggatat aatgatgatt aacttaacgg agagtagata
     3901 ttagaagtag tagaaggaca atttaagact ttatacccat taaaacaaag gggtaaaata
     3961 atgatgtttg atgaaaattt agaatgtgtt aatcacgaaa ttatcaatga aaaattagat
     4021 acgaaaattc accttgctaa tccttatgcc ttttgggggg gaagggtaaa tgagaatata
     4081 aatgggttaa ttaaacaaca ttctttaaga ggaactgact ttaataaaat ttttaaccgg
     4141 aagataaatt tttttgtaaa tggaataaat aattgccccc gaaaaactcg gaatataaaa
     4201 ccccctaatt aattatttaa aggaataaga gtttatttgc tcgtagagta attatgttcc
     4261 attaatatgc taaattgctc agtaaagtta ttaacttttt ttcaacgcca gtgaataagt
     4321 ctgttttatt aaataaggag ttcaggtgtt tatttcaaaa gaattatcaa gctttattgc
     4381 tgtagctaaa aataaatcaa taaataaagc aacaacggaa ctttttatta cacagtcacc
     4441 gatttgccga agcttaaaaa aactggaatt ttcattaggt gtaaaactct ttgtaagaaa
     4501 aagccaggga ttggctttaa cagaagaagg agaatgctta tataataaag ttttgtcaat
     4561 ttgtgaacgg cttcaagaaa tagaaggtga ctacaagaat gaaaaatcaa catacaatga
     4621 tctatatcaa aaaaatatat gaggttctaa tataataagc ggttctagat aactaaagtc
     4681 atctattacc aatatctgta tactcagtga aagttttgtc ttctaggtaa gttttcataa
     4741 tagtgacata ccttattttt tcatctgggt gtctctcatc ctgaagaatg ggtgatatag
     4801 ttgaaataac tcagttcgtt ttgtggttta cgatttttgg cggatcacaa aaatagggct
     4861 gagtcccttc aagtgagttt tgaaatatac aattataccc ttcatccttt aagttgttgc
     4921 tttgttggct gttttcattc acctcagtca catagttatc tatgctcctg aggattcgtt
     4981 cacttgccgt cgtgctgcaa ctcgaaatct attgggtata tatgtaattt tcatattctg
     5041 tttcatttta acggtattat ttaaatattt atatttagaa cattaggtta ttagaataga
     5101 taattttagg taaatgtaat taattcaacc atcacggaga gtttatcaat gttaaaatat
     5161 gctaatcctc agaccgtagc cacacaacgt actaaaaata ctgcgaagaa accgccatca
     5221 tcaacctctt ttgatgggca ccttgaactt tcaaatggtg aaaaccagcc ttacgaaggc
     5281 cataagatta ggaaaatcaa aggattgaga caacatcttg cggataggag tcttaataaa
     5341 gggcatattt ccccgttgat gaataaggga ttattagtag gtagtaagga tgtgtcaatt
     5401 gacatcccag ttatagctca tcgttatgac agcagccatc aacttacaga tgcggagcct
     5461 ttgaaagctg atagtcactc caatcatcta gacccttttt atggtgttat tgcaggattt
     5521 cgtggtgatc aggtgacatc atcagagtcg ggttcaggat caataggcgt acattggggg
     5581 aaaaatacgc tggattccaa tattatgggt gttaatgtgg ttaatggtgc atcgggaacc
     5641 gtgggaataa gaattgcttt gaaggatatt cagcatgggt ccccggttat tgtcacgtct
     5701 ggagcattga gcggttgtac tatggtatat tcggtgaaaa atggctattt ttttgcctat
     5761 catacggggc agaaaccggg taataatgaa tggaaaacag gcaggcaggg tgttgttgct
     5821 acctatctga gccatcaagc cctttcgccg gatagtgaac ctatgacggt tggtgaacag
     5881 aataacgatt tagtgaatat ttttgcaaat tatgatcaga gtgttattac ctatatggga
     5941 aaaccgggag tgctgattga taaaatggca gaaaatgttg gtgtttttaa ttatgacgaa
     6001 attaagcctg aaaaacctgc tattagagct ggctacagtt atgcgttgct ggctaaggac
     6061 gataaaggaa aggtgaacgt taaggtacta tcggaagatg ttatcgtttc atcaggtaag
     6121 cagggaaata ctgttaaggc cattaattca cttaaaaaac ggttgttata attttgccca
     6181 atgagggcag gaggtacgga ttttcattag cacttatcga agcggatata tgtttgtgac
     6241 tacattgtca gaaaatttca ttcatcaact tgaattagaa cagtgtccat ataacttatt
     6301 tagaataaag gaaggaaatt aatcttagat acaatgataa gggaaataaa atagaaagta
     6361 gagttaattg tattaataag gtgctggagc tttacttaca aaatatcaat gatttttggt
     6421 ttcgtataag taattaattt agagtaatga aaaattattt gtgggttaaa ttttatttat
     6481 tgaaaattca ttggtttatt ttatgaaata ggaaggtaat agcggtatta ttcggttgaa
     6541 ataaccgtat agataaatag taggatttag ataaattatt tcatatatga caatttttac
     6601 tattatatgg ggtttgtaag tcgaccgatg cccttgagag ccttcaaccc agtcagctcc
     6661 ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg
     6721 caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc
     6781 tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct
     6841 caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc
     6901 ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg
     6961 gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc
     7021 atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct
     7081 cttaccagcc taacttcgat cattggaccg ctgatcgtca cggcgattta tgccgcctcg
     7141 gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc
     7201 cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctgaatgga agccggcggc
     7261 acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg
     7321 tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc
     7381 gcacgcggcg catctcgggc agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc
     7441 tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac
     7501 cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa
     7561 catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct
     7621 gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt ggaacaccta
     7681 catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg tcccgccgca
     7741 tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt tcatcatcag
     7801 taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc atgaacagaa
     7861 atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt aacatggccc
     7921 gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg gacgcggatg
     7981 aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac cgcagctgcc
     8041 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     8101 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     8161 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
     8221 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     8281 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
     8341 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     8401 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8461 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8521 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8581 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8641 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8701 ctc
//
