LOCUS       sequence-388699-       13949 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter of mitochondrial matrix Ca2+..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13949)
  TITLE     Subcellular imaging of neuronal calcium handling in vivo
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pKK1 -pFlp-18::mito4x-GCaMP6f
FEATURES             Location/Qualifiers
     source          1..13949
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1498..1509)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    3575..3595
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             complement(6691..6702)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             7813..7887
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             7909..7983
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             8005..8079
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             8101..8175
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             8233..8250
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(8290..8298,8299..8313)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             8341..8397
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     complement(8998..9017)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     protein_bind    complement(10070..10090)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(10098..10115)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10098..10114)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11941..11963
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12001..12019)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12087..12191
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12192..12260,12261..13052)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12410..12429)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13223..13811
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13712..13731
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttcacaggg ccctgtcgga ttatgggctc ttcttttatc ctctactttc tttctagaaa
       61 atgcaaatgt tgagtcagaa attgctgaat ggagtacaac tgcaatgggt ttagcttatg
      121 tttctggaac aattactggt ggaattgtga ttgaaaagta aggttatatt tcatttcaat
      181 aaatgttacc acagtataac aaaaacaagt tacattgttc agagtatgtc tgaatgtatt
      241 attattattg tatttgtgca tcagtactgt agttgtactg aagaagggtt taaaataatc
      301 tcaacgtttt taagacgagt tagtgagaat acggtcgcag ttaggatgtt tggacatagt
      361 tttattagat aatcgtgaac taactcacta taaaaacccg ctaactcatt gaataagata
      421 gttttaaacg ttcaaaattt gaagacaaaa ttgtatgaat aagtttacag agttggtcga
      481 cgcaagcttc tccttctctt cacatttctc aataatttag ctctaattgc attcgtattc
      541 ttcgcaaaaa tacgtattct gattgacccg atgaaatacg gatgcctagt tgcattgatc
      601 atctacggtt atacttatgg gtgaggtttt tcaaatatat tttgatttaa tcgaataatg
      661 aaatttcaga actggagttg gtccaatttc gtggtttata tcctctgaac tggtacctca
      721 aaaacatcgt agtattgctc aatctgtggc atattcaatg aatactgtaa tggtggttat
      781 cagtacattt acagtattac ctctatattc cgttattggt agttatgctt tccttattct
      841 atattcaatt ccttcattta taagtatgct tatattattt cgctacttac cggaaaccaa
      901 aggacgagag attcatgata ttgtgaatga acttaaaaaa aaatgaaaac ctttgaaaac
      961 gttcatacgg ccgaaatttt acttgatcga gccaattgaa cttttgttaa gacttacaga
     1021 aaagtgagag attttgttac aaaaaagtga gaaaaaagcg aaaatgaaca ggaaaaagaa
     1081 gaaaaataca cccgaagtat catataaaaa ttaaaaagat tgtttgacct ggcggaactt
     1141 ttttgcaaaa actctgaata tccatggcac atgcttcgtg caaaacagga tccaaatgaa
     1201 tatcaacaag agattcttga agacgacgag ccacttgctc agagcattgc ttatctcgaa
     1261 tgagaccctt gttaaaatca tttttcaaac attatatgac attgtcgaaa tgtcctcctg
     1321 attccatgat ggtagcaaac tcacttcgtc tttgagccaa tttatccaag tccttgtaaa
     1381 aagtattcga aaattgttaa cggataaaat gtttattata atatcaaaaa caatttgtca
     1441 gttgaccact ttttgatata tttgacagaa accgggatga attggctcaa agttagggcg
     1501 cccttctatt acagggtttc tgataacaaa ccggttatta actcccaaca agggatgatt
     1561 tcaattcatc atgctcaaat tgacccaaat taagttacat gacaaattca tcgccctttt
     1621 caactcttct tggctcatca tctgttattc tgttcctatt ctgtctgcac cctatacccc
     1681 tttgcatact ctctcgtcat tcctttcttg gatagtgtct tcttctcccc agctctgcta
     1741 cttctatgac ttgcgcccgc tgcttttccg ctcgttcctc tcctctctga cgtcttcttc
     1801 tgcttctttt ctccttcctt tttcccttct caatcttcct ctttttccca tttctgtcaa
     1861 tcattcgaag aagaagaaga agaagaccct cattcatttc attttttttt ctgtcggtgt
     1921 gtgctgctcg gttaagagtg agctctctat tccacgtctt cttctttttc ttcttcgatt
     1981 cgaatcaatc actccaccaa aacgcattcg tttttgggat tcaccccgcg gttcgcaata
     2041 ggtttctttt tcaaattatt agcgttataa atagaaaaat ggtggagttt caataaaaat
     2101 gataatttac aaagtgtatt ttgattacat gtactcaaaa aggttgaaat ttttcatacc
     2161 agttttccgg aaatccatct gatatcatta tcgtattttt ctttttaaaa atgttttcaa
     2221 aaaaaaacaa aatatagctg gttatttggc accctctaat taccattttc ctgtcacacc
     2281 acactctttt ccttcttcca cttctttccg ttttcagccg cttccaacca aaccgatatg
     2341 aaagccgagc aacaacgatc gattccaccc ggctcggcga ccttcccgtc gcaggtgaga
     2401 ctcagaaaac tagagaaacc gctcaactaa ctcttgatat ccgatttcat tcttttctct
     2461 tttctttttg ttgaactttt ccatatttcc agatgccacg gccaccacca agcaccgaac
     2521 aaggaatcac aacggaatcg gagcttgcga agaaagctca aatcactccg aacgacgttt
     2581 tagcacttcc gggaatcact caaggtatct tgctcccttt ggatttcagt ttaaaacata
     2641 aatttaggat tcttatgctc cccatctgcg aacatctata acatcgagtt caccaagttc
     2701 caaatccgtg atctggacac tgagcaagtg cttttcgaga tcgccaaacc ggagaacgat
     2761 caggagaatg atgagtcgcc acaggagtcg gcaagatacg tgcgttatag atttgctcca
     2821 aactttttga aactcaaaac ggtcggagca actgtggaat tcaaagtagg agacatccca
     2881 atccatcatt tccgaatgat cgaacgtcac ttcttcaaag atcgccttct gaagtgtttt
     2941 gactttgaat tcggattctg tattccgaat tcacgaaaca actgtgaaca tatctatgag
     3001 ttccctcaac tctctcaaca actcagtgag tcattattct aaaaagtaca attcaaaaga
     3061 ctaatctctt tttcagtgga cgacatgatc aacaatccaa acgagactcg ttctgacagc
     3121 ttctatttcg tcgataacaa actcgtcatg cacaacaaag ccgactactc atatgatgca
     3181 taaatattta atacaaaaat gttctggata attattctgt cgaatagaaa aaaaactcca
     3241 aatgtgatta aattccaata attcctgtct agtttgttcc ttccccttcc ccttctcatg
     3301 ttcaatgcat tcctaagctt ttcagttccc cccttgtttc tatatttttt cgctgtcctg
     3361 tcacactcgc taaaaacact aatcacacgg aaatctgttt tcaataaaaa ctccaacttt
     3421 aactcatttt caatttcaac tgaaagattt tttcattaga gaatgtctag aactaggccc
     3481 gggctacgta atacgactca cttaaggcct tgactagagg gtaccagagc tcacctaggc
     3541 aggaacagct atgaccatga ttacgccaag ctatcaactt tgtatagaaa agttgcgagt
     3601 ttgttggagc aaaacacgga acactagtgc tagagcgtca atttttgagc taaaacaaat
     3661 ctgtcacata ctgctcgaat cggaggtagg tttgaaaaac atgcaattca tttccaaaat
     3721 atgacgaatt ttgaaaaaaa aaatagaaaa gaaactcaat taaattacct tattttttct
     3781 agtacgtcaa ttttgacttg cacttaaaat ctgcatttta aaaatgaaaa ttcagtgcag
     3841 ctgcagtgca gtgcggctat tattttcggc atttttgcaa aacttacaca ccaccgttat
     3901 aagaaaatat ttgtcttttt tttaaatttt tattcagtcg ttattcgaaa aagacttaca
     3961 gtaccttgct gaattccttc cgcattgcgc taaaatttac cgattgcaaa ataatcttac
     4021 atgctatagt aataaaaaat ttacatactt gccatttcat ttcatttaat tatttcatcc
     4081 aatttatctt gatcagtatt actctttgtt aaaaattcca ctgttcaatt tttacatttc
     4141 agttgtttta atatcgaagt gcacaagaag cgcgaacata atggacacca tcgctcaaaa
     4201 attatgcttc tagttgtagt tcctcgagca tttttgatct caaaagacat ttccaagagc
     4261 aataattgcc atttttgagc tacaagtacc atatcctgct cataattcat gttccaccac
     4321 tccctaaagt tttttctttg gaattcccca gactttgcgc ctaaaattct cgttattctc
     4381 tccaattcaa ttggctcaat tcaactgact agatttcagt gatgtaggta gcgcggaact
     4441 cagtattaag agaggactca actcagtttc taatccaatt tcaacaaatt ctaagacacc
     4501 gaaacgctgc aggtctcgta gagcctacgt gttgacaggt tcttcctttt caatctattt
     4561 ccgtcgatat tccggcgtca tttagttgaa aatgagtgaa aaaatgcgat ctgacgcgaa
     4621 ttcacgtgaa ctcgaaaatt cgagaatgta atcattaagt ttccaaacac caagttctct
     4681 ttcgaaacca agtcaataga aaaatgagac acccacgaga agaaggttgg tccgaaaaaa
     4741 gaaactaaag aagttattta tttctctttt tgatgtttgt tgagcagaat atgatttagt
     4801 taagcacatc tgatttcaac gccgatgaat gaggtcgtag gcgtcacgtt caatttgatc
     4861 tgatttgttc agtgcttacg agtagaaatt ctaaatatga tgttactttt tggttctgta
     4921 agatggagct gggtatacat gtggtgcacg gcaatcgcta tttggcaatt tcagtcattt
     4981 cggcaaattc ggcaattttg gcgaattctc atattttgga aattttggca aatgcggaaa
     5041 atttcaaaaa caagcaatta ccaaatttgc caaacgccga aaaatttaaa aaaaaaccga
     5101 catttgctgg tatttttttt taatcaggat gctaattatg taaggttaat ctataaattc
     5161 ttgaaacaca cttaactttg aaattgaaat cactgacatt tcgcgcattc gccgataaaa
     5221 tctctaaata aaataatgta aactgtgtcc agttttggta cttgcttagt atacctggtt
     5281 caaaatattt tccaatgcgt tttccattta tttattttag aaatgatcat ttaattaaaa
     5341 cgtaaaattc gcattttcaa gcttagaaac attaatccta agtccaatat ggacaaaaag
     5401 agtagtgtat tagtaccaag taagaaccct aaaaaaaaaa tattctaatt aaatttttgg
     5461 caaaacgcca catttttctt ttttttctca acggggaaaa gttgtgtttc ttcccttttt
     5521 attaaatttt ttgttttcct taggctaagt acaacttgaa atggaacaga aattacataa
     5581 atttattgct ttcacatttt gctcttcttc actcaaatct tggactcttg cttttcacaa
     5641 aaaaaacctt ataaacaaaa ataagacatc tgaaaaaatg atgaacttcc tgtcagtttg
     5701 ttccagtatc cttcttaaat ttccttgttt tccacatcac aaaaatccta catcacgctt
     5761 cgactcattt cctgacagtc atcacatcac caaatgaatt tctattttta caatatagtt
     5821 gtagtatttt tgtcgaacat atattgttct aactaacttc ttaaaaaatt gtgctcttga
     5881 ctgaacacaa atttctgcgt cgtttcacgt ttacttttct caatttttta aaaccaaaat
     5941 ttaactgcaa acttctgcat tgttctaaaa actcacaggt tatctcaaaa agataaattt
     6001 caaagtgaga tttttttaaa aaaattccgg actgtttttt gattttgatg tttttaatga
     6061 atttttaaaa ttctttatta gtgcctgaaa ttaacaataa ttttaaagtt tgtcacactg
     6121 cgaataaatt aatctcacat ttcgtatgtt tagaaagatg catgaaactc cgtcgttctt
     6181 atgaagttaa ttaagacatg gctaattgaa agcccacatg gtcatgaaaa agtaaaactt
     6241 acaacttttt actgaatatt aaccagtctc tataaatatc ccagcttagc cggaataggg
     6301 tcacgaaaac atgaaaataa agcttgaacc tattgttatg attttcaatg ccaccttttg
     6361 cagtttaata tacactatta ctacttctca gttcaagttc acttctgaga cggaaccttt
     6421 attttctaac tttctttaat cttacttttt atgagccgaa tgttctattt tgtaaaatga
     6481 tcagatacaa gcccaaagct aattttgcca ataagtgtta gtagatcaat tgaattacaa
     6541 tataatgacc tgggcggaat agatttttgc actgattttt ttccaagctt actctatgaa
     6601 tattcataga tcatcttcaa aggtagcgtg tgttgtagtt gtgttttggc gggttcgtgt
     6661 tatttgactc agctcaagta gatgttgtag tcttccctca atcaacgcgt gggcttgacc
     6721 ctctttaaat tgagtggaaa ccagccaatc tatttgaaat ctacccccga aatgagattc
     6781 gacgatgaca cgacgtgtgt gttacgtgcg ctgagaattt aggaaaaaaa agataatctt
     6841 cttttcgtta gctgcgccac aacctgtgca gataaactcc gcacaattga agttcttact
     6901 ggtcccaccc gatttattca attgtattgt gtcttttttt tttatttctc tacaactcta
     6961 actttcttca actattctct acatcagtgc ttttttccac catgaaaaaa gaaatgacgt
     7021 cgttgtttct taggaggggc ggagcctcaa ctcaatggtc cgttcgaata gatcccaccc
     7081 ctcatataaa taggactgaa accaaagaag tttttaaatt ttcagcttac cgtgtttctc
     7141 aattttcaaa atagtctttt ttgtgtctga acgggctgat caattatgtt tttttttgaa
     7201 tgaaaaatct tcctcccaag ccttaaaatt ccttccaaag atcgaaagtt atgtctattc
     7261 aagttacttc ttcttagatc aaatattaaa caaaaaaata cgccatcatt ctctcacatt
     7321 tagtaattca aaatttcgat ttgttttcga acttttgaat ttcccgccta ggaagtgaca
     7381 tgagtgaaat ctcattagat aaatgtgcca ctcaccgatg acactatttt caaataaggg
     7441 ggggggtgtt tcgaatacct tgtacacgcg tttccgcaaa accacacacc ccgagttcct
     7501 cagcttcttc ccctcgaccc atgagccacg tcaagatgtc agcgtgtcac ttttcattgc
     7561 ttcaccttct acggtaacga gaaaaggcgg ggaatccagg atctggtgtg acttcttcct
     7621 tcttcgtatc ttcctaaaaa tgttcgttta ctttttagcg agtgtcattt gatgggatgt
     7681 cgttcttgag gaaagaggct agaagcttaa tactctcata tttcatatta ttggcatcaa
     7741 gacgtttatt atttaactac aataacgatt gctttaacaa ctaaaaataa taatttcagg
     7801 gttagacatg acatgagcgt gctgacacct ctgctgctga gaggcctgac aggctctgct
     7861 agaaggctgc ctgtgcctag agccaagatc cactctctgg gcgaccctat gtctgtgctg
     7921 actccactgc tcctgcgggg actgactgga tctgctcgta gactgccagt gccacgggct
     7981 aagattcaca gcctgggcga tccaatgagt gtgcttaccc cactcctgct cagaggactg
     8041 accggaagtg ctagacgcct gccagttcca agggccaaaa tccatagtct gggagatccc
     8101 atgtccgtgt tgacacccct cttgctgagg ggacttaccg gctcagctcg gagactccct
     8161 gttccaagag caaagattca ttccctcggc gacccaagat ctggcagcgc taaagaccct
     8221 accatgggta gccaccacca ccatcaccac ggcatggctt ctatgacagg cggccagcag
     8281 atgggccgcg acctgtacga tgacgacgat aaggacctgg ccacaatggt ggacagcagc
     8341 aggcggaagt ggaacaagac aggccacgcc gttagagcca tcggcagact gtctagcctg
     8401 gaaaacgtgt acatcaaggc cgacaagcag aagaacggca tcaaggctaa cttcaagatc
     8461 cggcacaaca tcgaggacgg cggagtgcag ctggcttacc actaccagca gaacacccct
     8521 atcggcgacg gaccagttct gctgcctgac aaccactacc tgagcgtgca gagcaagctg
     8581 agcaaggacc ccaacgagaa gagggaccac atggtgctgc tggaatttgt gaccgccgct
     8641 ggcatcacac tcggcatgga cgaactgtac aaaggcggca caggcggctc tatggtgtcc
     8701 aagggcgaag aactgttcac cggcgtggtg cccattctgg tggaactgga tggggatgtg
     8761 aacggccaca agttcagcgt cagcggagaa ggcgaaggcg acgccacata cggaaagctg
     8821 accctgaagt tcatctgcac caccggcaaa ctgcccgtgc cttggcctac actggtcacc
     8881 acactgacat acggcgtgca gtgcttcagc agataccccg accatatgaa gcagcacgac
     8941 ttcttcaaga gcgccatgcc tgagggctac atccaagaga gaaccatctt ctttaaggac
     9001 gacggcaact acaagaccag ggccgaagtg aagttcgagg gcgacaccct ggtcaacaga
     9061 atcgagctga agggcatcga cttcaaagag gacggcaaca tcctgggcca caagctcgag
     9121 tacaacctgc ctgaccagct gaccgaggaa cagatcgccg agttcaaaga agagttcagc
     9181 ctgttcgaca aggacggcga cggcacaatc accaccaaag aactgggcac cgtgatgaga
     9241 agcctgggac agaaccctac agaggccgag ctgcaggaca tgatcaacga ggttgacgct
     9301 gatggcgacg gaactatcga cttccccgag ttcctgacca tgatggccag aaagatgaag
     9361 tacagggaca cagaggaaga gatccgcgag gccttcggcg tgttcgataa ggatggaaac
     9421 ggctacatct ctgccgctga gctgagacac gtgatgacca acctgggcga gaagctgaca
     9481 gacgaagagg tggacgagat gatcagagag gccgacattg acggcgacgg gcaagtgaac
     9541 tacgaagagt ttgtccagat gatgaccgcc aagtgaaccg gtgtcgacga attcgggccc
     9601 gtttaaaccc gctgatcagc ttgtacaaag tggttggatg atcgacgcca acgtcgttga
     9661 attttcaaat tttaaatact gaatatttgt tttttttcct attatttatt tattctcttt
     9721 gtgttttttt tcttgctttc taaaaaatta attcaatcca aatctaaaca tttttttttc
     9781 tctttccgtc tcccaattcg tattccgctc ctctcatctg aacacaatgt gcaagtttat
     9841 ttatcttctc gctttcattt cattaggacg tggggggaat tggtggaagg gggaaacaca
     9901 caaaaggatg atggaaatga aataaggaca cacaatatgc aacaacattc aattcagaaa
     9961 tatggaggaa ggtttaaaag aaaacataaa aatatataga ggaggaagga aaactagtaa
    10021 aaaataagca aagaaattag gcgaacgatg agaattgtcc tcgcttggac aactttatta
    10081 tacatagttg ataattcact ggccgtcgtt ttacactcga gacgtacggt gcgcgcgatg
    10141 cattcgaaga tctgcccact agtgagtcgt attataagtg caagtaagat cagtgtttgt
    10201 ttcgatatca gtctgtttcg taacggtctt ctgtataact acaaaaaaga ataaaaaacc
    10261 gtatcacaac tgagaaaaaa gacaaaactt attgaacagt gttcctttca aaaaattatt
    10321 ttgtaaaaaa tcgagttttt ctccaaaata acgcacgcaa tcaaaaacaa gaaaactaac
    10381 gtgttctcct gggcgtggaa ctccttatca agattaccat tattgtctgt cagaattgat
    10441 atttcaatta tttcatatct agacaaaccc gggtgtcttg catttattag tactgtgagt
    10501 ttacatacaa acgaggctta ctcagataac atagagtcac ctctaattag caaaaaccca
    10561 tttctaatta gctatttcga ccagttctaa agtcaccgta attagaaatg gtttttgtag
    10621 cttttctaat tagaaatgac cccgagataa gtgaaatatg gcattttctg ggttttctag
    10681 taagaaatga tcccgcacgt agtgattccg cgttacaagc caaattcaaa ttaaaaaagt
    10741 atattacaca attcaagtta cattattttc aggaaacaat ggcgaagagc aaagcagaga
    10801 ttcaaaagga ggaaagagac aacagatatg aacgaagact tcttgaaaag agaaagagac
    10861 atgaggtgag taaccatgat aataaataat caagtaaggg ctatagattt ataaattctg
    10921 gagttaacag tttcgaaaaa aagtagtttt catatgtgcg aaggaatatc aaaaataggg
    10981 gaatagccta aacgagtgag aaaattaaat gtttgttatt ttttcaaaaa tgtctgcagg
    11041 gccgttatcc tttcaaatgc actctatcaa acatagatca tatccatcat atcttatcca
    11101 gaatttatat catatccaag attgagagcg tttcaaggaa aatctcaaac aaccatacaa
    11161 ttttgtagat cgacaacaag gcctccactt cttcatcgat ggcctctgca gcacctgcac
    11221 caccaagttt gtatttttca tattaatata aagtgagcca cgcatagtcg tttttaggat
    11281 ttttgccaga tcgatgtcgc gcaaacagac tgtttttttt tctttgaaag ccgtgcgtga
    11341 catgggccac ggattgtaac gccgcgctgg atggataaaa atttattcag ttttttagcc
    11401 tttacaaaat atggttcgac tactttcttt atttttacat ttttataggt gctcattgag
    11461 ctgagatatt ttgggtataa tcggaaaatc gctgattttg gtcatttttc tgcactaaag
    11521 attacacaaa aagttcagta tttagattca ttgttgaaaa ttggggatga tctacagata
    11581 catatcgccg tgttcaaaaa cacacgtgct gtgacgtcgt gactggggaa aaccctggcg
    11641 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
    11701 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
    11761 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
    11821 gtgcaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
    11881 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
    11941 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
    12001 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
    12061 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
    12121 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
    12181 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
    12241 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
    12301 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
    12361 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
    12421 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
    12481 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
    12541 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
    12601 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
    12661 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    12721 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    12781 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    12841 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    12901 agcgtgggtc tcgcggtatc attgcagcac tggggtcaga tggtaagccc tcccgtatcg
    12961 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    13021 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    13081 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    13141 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    13201 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    13261 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    13321 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
    13381 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    13441 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    13501 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    13561 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    13621 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    13681 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    13741 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    13801 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggtcttt tgctggcctt
    13861 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    13921 ttgagtgagc tgataccagc ggataacaa
//
