LOCUS       sequence-388730-        2737 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for overexpression of mutated initiator tRNA (A37G).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Hollerer S, Jeschek M
  TITLE     Ultradeep characterisation of translational sequence determinants 
            refutes rare-codon hypothesis and unveils quadruplet base pairing of
            initiator tRNA and transcript.
  JOURNAL   Nucleic Acids Res. 2023 Mar 21;51(5):2377-2396. doi: 
            10.1093/nar/gkad040.
  PUBMED    36727459
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2023-03-21- 21"; volume: "51"; issue: 
            "5"; pages: "2377-2396"
COMMENT     Sequence Label: ptRNAfMet-A37G
FEATURES             Location/Qualifiers
     source          1..2737
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..31
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     terminator      714..808
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        849..951
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             952..1611
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1018..1037)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     oriT            1767..1875
                     /note="incP origin of transfer"
     rep_origin      1922..2467
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     2368..2387
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2584..2601
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2645..2731)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt accaaaataa caccctgctt aattaactga tgatgagcct ggatttccgc
      121 tctcactgaa tttttatgca aaataaatga gttttcattt aatcatcttt tatcggagac
      181 aggaagagtt tagtgtgttt tttgtaaaat aatgcgctta agggagagca ggagaaggca
      241 aaagtattca acaaatgaaa gtgaactgga tattcattca catgattagc aataaacgtt
      301 gacaaaatgt ggcgtggatc actataatgc ctgcagattt tacgtcccgt ctcggtacac
      361 caaatcccag cagtatttgc attttttacc caaaacgagt agaatttgcc acgtttcagg
      421 cgcggggtgg agcagcctgg tagctcgtcg ggctcatgac ccgaaggtcg tcggttcaaa
      481 tccggccccc gcaaccactt tcccttagag tcctttttca aatatactgt gaagacttcg
      541 gccttcgtag tgggatttga aaaaatcctt ctggaaagtg ctccagaccg cagttgcggt
      601 tatagggttc agttatataa agccccgatt tatcggggtt ttttgttatc tgactacaga
      661 ataactgggc tttaggccct ttttttatgt cttgggggtg ggcactagtc ttggactcct
      721 gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg
      781 ccgccgggcg ttttttattg gtgagaatcc aggggtcccc aataattacg atggcgaaaa
      841 tgagacgttg atcggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta
      901 ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa aatggagaaa
      961 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
     1021 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
     1081 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
     1141 gcccgcctga tgaatgctca tccggaattt cgtatggcaa tgaaagacgg tgagctggtg
     1201 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
     1261 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
     1321 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
     1381 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
     1441 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg
     1501 ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga
     1561 atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta atttgacttt
     1621 cgcgcgttgt ccttttccgc tgcataaccc tgcttcgggg tcattatagc gattttttcg
     1681 gtatatccat cctttttcgc acgatataca ggattttgcc aaagggttcg tgtagacttt
     1741 ccttggtgta tccaacggcg tcagccgggc aggataggtg aagtaggccc acccgcgagc
     1801 gggtgttcct tcttcactgt cccttattcg cacctggcgg tgctcaacgg gaatcctgct
     1861 ctgcgaggct ggccgtaggc cggccctaga aatattttat ctgattaata agatgatctt
     1921 cttgagatcg ttttggtctg cgcgtaatct cttgctctga aaacgaaaaa accgccttgc
     1981 agggcggttt ttcgaaggtt ctctgagcta ccaactcttt gaaccgaggt aactggcttg
     2041 gaggagcgca gtcaccaaaa cttgtccttt cagtttagcc ttaaccggcg catgacttca
     2101 agactaactc ctctaaatca attaccagtg gctgctgcca gtggtgcttt tgcatgtctt
     2161 tccgggttgg actcaagacg atagttaccg gataaggcgc agcggtcgga ctgaacgggg
     2221 ggttcgtgca tacagtccag cttggagcga actgcctacc cggaactgag tgtcaggcgt
     2281 ggaatgagac aaacgcggcc ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac
     2341 aggagagcgc acgagggagc cgccaggggg aaacgcctgg tatctttata gtcctgtcgg
     2401 gtttcgccac cactgatttg agcgtcagat ttcgtgatgc ttgtcagggg ggcggagcct
     2461 atggaaaaac ggctttgccg cggccctctc acttccctgt taagtatctt cctggcatct
     2521 tccaggaaat ctccgccccg ttcgtaagcc atttccgctc gccgcagtcg aacgaccgag
     2581 cgtagcgagt cagtgagcga ggaagcggaa tatatccggc gcgcccagct gtctagggcg
     2641 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     2701 agtctttcga ctgagccttt cgttttattt gatgcct
//
