LOCUS       sequence-388790-        1167 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Eukaryotic expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1167)
  TITLE     Eukaryotic expression plasmid with a puromycin selection marker
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1-(-)-Pur
FEATURES             Location/Qualifiers
     source          1..1167
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    720..841
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(757..776)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     811..830
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(890..906)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(890..906)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(903..925)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    914..930
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(938..944,945..962,963..968))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    983..1004
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1121..1138)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aagtgcgcct gggccactcg cgatgatgtg ccccgcgccg tccgcactct ggccgccgct
       61 ttcgccgact accccgctac ccggcacacc gtggaccccg accggcacat cgagcgtgtg
      121 acagagttgc aggagctgtt cctgacccgc gtcgggctgg acatcggcaa ggtgtgggta
      181 gccgacgacg gcgcggccgt ggccgtgtgg actacccccg agagcgttga ggccggcgcc
      241 gtgttcgccg agatcggccc ccgaatggcc gagctgagcg gcagccgcct ggccgcccag
      301 cagcaaatgg agggcctgct tgccccccat cgtcccaagg agcctgcctg gtttctggcc
      361 actgtaggag tgagccccga ccaccagggc aagggcttgg gcagcgccgt cgtgttgccc
      421 ggcgtagagg ccgccgaacg cgccggtgtg cccgcctttc tcgaaacaag cgcaccaaga
      481 aaccttccat tctacgagcg cctgggcttc accgtgaccg ccgatgtcga ggtgcccgag
      541 ggacctagga cctggtgtat gacacgaaaa cctggcgcct aaggcgcgcc cacgtgctac
      601 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
      661 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
      721 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
      781 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
      841 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
      901 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
      961 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     1021 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     1081 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     1141 cctcggtcgt tcggctgcgg caaacgg
//
