LOCUS       sequence-388900-        1039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GCaMP8f in a Cre-dependent manner.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Moffa JC, Bland IN, Tooley JR, Kalyanaraman V, Heitmeier M, Creed 
            MC, Copits BA
  TITLE     Cell-Specific Single Viral Vector CRISPR/Cas9 Editing and 
            Genetically Encoded Tool Delivery in the Central and Peripheral 
            Nervous Systems.
  JOURNAL   eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.2024. doi: 
            10.1523/ENEURO.0438-23.2024. Print 2024 Jul.
  PUBMED    38871457
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/ENEURO.0438-23.2024"; journalName: "eNeuro"; date: 
            "2024-07-5- 5"; volume: "11"; issue: "7"; pages: 
            "ENEURO.0438-23.2024"
COMMENT     Sequence Label: pX552-hsyn DIO GCaMP8f(with gRNA scaffold)
FEATURES             Location/Qualifiers
     source          1..1039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..105
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    176..209
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             219..236
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     518..539
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(645..666)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(906..925)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gggnnnngnn nncncccgta gatctctcga gcagcgctcg gtatcgataa gcttgaacct
       61 cttcgaggga cataacttcg tataatgtat gctatacgaa gttatgggtc gatggtgaga
      121 tctggactag agggtcgatg gtgatgcttg gatcaaaata ggaagaatcc caatgataac
      181 ttcgtataaa gtatcctata cgaagttatg atatcatgca tcatcaccat catcacacgc
      241 gtcgcaagaa gaccttcaag gaggtggcca ccgccgtgaa gatcatcgcc cgcctgatgg
      301 gcctgaagat caacgtctat atcaaggccg acaagcagaa gaacggcatc aaggcgaact
      361 tccacatccg ccacaacatc gaggacggcg gcgtgcagct cgcctaccac taccagcaga
      421 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcgtggagt
      481 ccaaactttc gaaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
      541 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggcggtacc ggagggagca
      601 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
      661 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg tgagggcgat gccacctacg
      721 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
      781 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
      841 agcacgactt cttcaagtcc gccatgcccg aannctacat ccaggagcgc accatcttct
      901 tcnangacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgn
      961 gaaccgcatc gagctgaann natcgacttc nnnnnnnggc acatcctggg gcacnagctg
     1021 gagtacnanc tgccggacc
//
