LOCUS       sequence-388905-       13491 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human codon-optimized SpRY-ABE8e(V106W) and blasticidin 
            resistance: EFS promoter-SpRY-ABE8e(V106W)-NLS-FLAG-P2A-BSD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13491)
  AUTHORS   Kim N, Choi S, Kim S, Song M, Seo JH, Min S, Park J, Cho SR, Kim HH
  TITLE     Deep learning models to predict the editing efficiencies and 
            outcomes of diverse base editors.
  JOURNAL   Nat Biotechnol. 2024 Mar;42(3):484-497. doi: 
            10.1038/s41587-023-01792-x
  PUBMED    37188916
REFERENCE   2  (bases 1 to 13491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2024-03"; volume: "42"; issue: "3"; pages: 
            "484-497"
COMMENT     Sequence Label: SpRY-ABE8e(V106W)
FEATURES             Location/Qualifiers
     source          1..13491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(629..1420,1421..1489))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1252..1271
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1490..1594)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1669..1688)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1860..2239
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2241..2439
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2394..2414
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2457..2637
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2684..2809
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3302..3535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3720..3764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4062..4179
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4272..4483
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             4534..4554
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5149..9249
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAERTRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAKQLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTRLGAPRAFKYFDTTIDPKQYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             9292..9312
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9313..9336
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             9346..9402
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9403..9801
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9826..10414
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9879..9899)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10297..10308)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10417..10433)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10418..10434)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10939..11119
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(11145..11162)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11151..11375
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11421..11849
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11508..11527)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11718..11739
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11858..11878)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      12023..12157
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12084..12103
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        12219..12266
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             12285..12659
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    12789..12910
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12826..12845)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12880..12899
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12959..12975)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12959..12975)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12972..12994)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12983..12999
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13007..13013,13014..13031,13032..13037))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13052..13073
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13190..13207)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(13361..13491,1..458))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13441..13460)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
       61 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
      121 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
      181 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
      241 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
      301 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
      361 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
      421 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
      481 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
      541 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
      601 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
      661 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
      721 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
      781 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
      841 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
      901 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
      961 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     1021 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     1081 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     1141 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     1201 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     1261 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     1321 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     1381 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     1441 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     1501 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     1561 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     1621 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
     1681 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
     1741 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
     1801 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
     1861 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     1921 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     1981 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     2041 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     2101 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     2161 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     2221 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     2281 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     2341 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     2401 gggcggtagg cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt
     2461 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     2521 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     2581 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     2641 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     2701 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     2761 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     2821 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     2881 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     2941 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     3001 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     3061 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     3121 caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata
     3181 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     3241 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     3301 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     3361 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     3421 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     3481 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     3541 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     3601 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     3661 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     3721 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     3781 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     3841 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     3901 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
     3961 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc
     4021 gtgcgccaat tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg
     4081 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     4141 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
     4201 agagatccag tttggttaat tagctagcta ggtcttgaaa ggagtgggaa ttggctccgg
     4261 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
     4321 cggcaattga tccggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
     4381 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
     4441 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggaccggt tgccaccatg
     4501 aaacggacag ccgacggaag cgagttcgag tcaccaaaga agaagcggaa agtctctgag
     4561 gtggagtttt cccacgagta ctggatgaga catgccctga ccctggccaa gagggcacgg
     4621 gatgagaggg aggtgcctgt gggagccgtg ctggtgctga acaatagagt gatcggcgag
     4681 ggctggaaca gagccatcgg cctgcacgac ccaacagccc atgccgaaat tatggccctg
     4741 agacagggcg gcctggtcat gcagaactac agactgattg acgccaccct gtacgtgaca
     4801 ttcgagcctt gcgtgatgtg cgccggcgcc atgatccact ctaggatcgg ccgcgtggtg
     4861 tttggatgga gaaattctaa aagaggcgcc gcaggctccc tgatgaacgt gctgaactac
     4921 cccggcatga atcaccgcgt cgaaattacc gagggaatcc tggcagatga atgtgccgcc
     4981 ctgctgtgcg atttctatcg gatgcctaga caggtgttca atgctcagaa gaaggcccag
     5041 agctccatca actccggagg atctagcgga ggctcctctg gctctgagac acctggcaca
     5101 agcgagagcg caacacctga aagcagcggg ggcagcagcg gggggtcaga caagaagtac
     5161 agcatcggcc tggccatcgg caccaactct gtgggctggg ccgtgatcac cgacgagtac
     5221 aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg accggcacag catcaagaag
     5281 aacctgatcg gagccctgct gttcgacagc ggcgaaacag ccgagcggac ccggctgaag
     5341 agaaccgcca gaagaagata caccagacgg aagaaccgga tctgctatct gcaagagatc
     5401 ttcagcaacg agatggccaa ggtggacgac agcttcttcc acagactgga agagtccttc
     5461 ctggtggaag aggataagaa gcacgagcgg caccccatct tcggcaacat cgtggacgag
     5521 gtggcctacc acgagaagta ccccaccatc taccacctga gaaagaaact ggtggacagc
     5581 accgacaagg ccgacctgcg gctgatctat ctggccctgg cccacatgat caagttccgg
     5641 ggccacttcc tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga caagctgttc
     5701 atccagctgg tgcagaccta caaccagctg ttcgaggaaa accccatcaa cgccagcggc
     5761 gtggacgcca aggccatcct gtctgccaga ctgagcaaga gcagacggct ggaaaatctg
     5821 atcgcccagc tgcccggcga gaagaagaat ggcctgttcg gaaacctgat tgccctgagc
     5881 ctgggcctga cccccaactt caagagcaac ttcgacctgg ccgaggatgc caaactgcag
     5941 ctgagcaagg acacctacga cgacgacctg gacaacctgc tggcccagat cggcgaccag
     6001 tacgccgacc tgtttctggc cgccaagaac ctgtccgacg ccatcctgct gagcgacatc
     6061 ctgagagtga acaccgagat caccaaggcc cccctgagcg cctctatgat caagagatac
     6121 gacgagcacc accaggacct gaccctgctg aaagctctcg tgcggcagca gctgcctgag
     6181 aagtacaaag agattttctt cgaccagagc aagaacggct acgccggcta cattgacggc
     6241 ggagccagcc aggaagagtt ctacaagttc atcaagccca tcctggaaaa gatggacggc
     6301 accgaggaac tgctcgtgaa gctgaacaga gaggacctgc tgcggaagca gcggaccttc
     6361 gacaacggca gcatccccca ccagatccac ctgggagagc tgcacgccat tctgcggcgg
     6421 caggaagatt tttacccatt cctgaaggac aaccgggaaa agatcgagaa gatcctgacc
     6481 ttccgcatcc cctactacgt gggccctctg gccaggggaa acagcagatt cgcctggatg
     6541 accagaaaga gcgaggaaac catcaccccc tggaacttcg aggaagtggt ggacaagggc
     6601 gcttccgccc agagcttcat cgagcggatg accaacttcg ataagaacct gcccaacgag
     6661 aaggtgctgc ccaagcacag cctgctgtac gagtacttca ccgtgtataa cgagctgacc
     6721 aaagtgaaat acgtgaccga gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa
     6781 aaggccatcg tggacctgct gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa
     6841 gaggactact tcaagaaaat cgagtgcttc gactccgtgg aaatctccgg cgtggaagat
     6901 cggttcaacg cctccctggg cacataccac gatctgctga aaattatcaa ggacaaggac
     6961 ttcctggaca atgaggaaaa cgaggacatt ctggaagata tcgtgctgac cctgacactg
     7021 tttgaggaca gagagatgat cgaggaacgg ctgaaaacct atgcccacct gttcgacgac
     7081 aaagtgatga agcagctgaa gcggcggaga tacaccggct ggggcaggct gagccggaag
     7141 ctgatcaacg gcatccggga caagcagtcc ggcaagacaa tcctggattt cctgaagtcc
     7201 gacggcttcg ccaacagaaa cttcatgcag ctgatccacg acgacagcct gacctttaaa
     7261 gaggacatcc agaaagccca ggtgtccggc cagggcgata gcctgcacga gcacattgcc
     7321 aatctggccg gcagccccgc cattaagaag ggcatcctgc agacagtgaa ggtggtggac
     7381 gagctcgtga aagtgatggg ccggcacaag cccgagaaca tcgtgatcga aatggccaga
     7441 gagaaccaga ccacccagaa gggacagaag aacagccgcg agagaatgaa gcggatcgaa
     7501 gagggcatca aagagctggg cagccagatc ctgaaagaac accccgtgga aaacacccag
     7561 ctgcagaacg agaagctgta cctgtactac ctgcagaatg ggcgggatat gtacgtggac
     7621 caggaactgg acatcaaccg gctgtccgac tacgatgtgg accatatcgt gcctcagagc
     7681 tttctgaagg acgactccat cgacaacaag gtgctgacca gaagcgacaa gaaccggggc
     7741 aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga tgaagaacta ctggcggcag
     7801 ctgctgaacg ccaagctgat tacccagaga aagttcgaca atctgaccaa ggccgagaga
     7861 ggcggcctga gcgaactgga taaggccggc ttcatcaaga gacagctggt ggaaacccgg
     7921 cagatcacaa agcacgtggc acagatcctg gactcccgga tgaacactaa gtacgacgag
     7981 aatgacaagc tgatccggga agtgaaagtg atcaccctga agtccaagct ggtgtccgat
     8041 ttccggaagg atttccagtt ttacaaagtg cgcgagatca acaactacca ccacgcccac
     8101 gacgcctacc tgaacgccgt cgtgggaacc gccctgatca aaaagtaccc taagctggaa
     8161 agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc
     8221 gagcaggaaa tcggcaaggc taccgccaag tacttcttct acagcaacat catgaacttt
     8281 ttcaagaccg agattaccct ggccaacggc gagatccgga agcggcctct gatcgagaca
     8341 aacggcgaaa ccggggagat cgtgtgggat aagggccggg attttgccac cgtgcggaaa
     8401 gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc
     8461 agcaaagagt ctatccggcc caagaggaac agcgataagc tgatcgccag aaagaaggac
     8521 tgggacccta agaagtacgg cggcttcctg tggcccaccg tggcctattc tgtgctggtg
     8581 gtggccaaag tggaaaaggg caagtccaag aaactgaaga gtgtgaaaga gctactgggg
     8641 atcaccatca tggaaagaag cagcttcgag aagaatccca tcgactttct ggaagccaag
     8701 ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc ctaagtactc cctgttcgag
     8761 ctggaaaacg gccggaagag aatgctggcc tctgccaagc agctgcagaa gggaaacgaa
     8821 ctggccctgc cctccaaata tgtgaacttc ctgtacctgg ccagccacta tgagaagctg
     8881 aagggctccc ccgaggataa tgagcagaaa cagctgtttg tggaacagca caagcactac
     8941 ctggacgaga tcatcgagca gatcagcgag ttctccaaga gagtgatcct ggccgacgct
     9001 aatctggaca aagtgctgtc cgcctacaac aagcaccggg ataagcccat cagagagcag
     9061 gccgagaata tcatccacct gtttaccctg acccggctgg gagcccctcg ggccttcaag
     9121 tactttgaca ccaccatcga ccccaagcag taccggagca ccaaagaggt gctggacgcc
     9181 accctgatcc accagagcat caccggcctg tacgagacac ggatcgacct gtctcagctg
     9241 ggaggcgact ctggcggctc aaaaagaacc gccgacggca gcgaattcga gcccaagaag
     9301 aagaggaaag tcgattacaa agacgatgac gataagggat ccggcgcaac aaacttctct
     9361 ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggccaa gcctttgtct
     9421 caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct
     9481 gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc
     9541 aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct
     9601 gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc
     9661 ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc
     9721 atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct
     9781 ggttatgtgt gggagggcta agaattcgat atcaagctta tcggtaatca acctctggat
     9841 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     9901 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     9961 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
    10021 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
    10081 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
    10141 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
    10201 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
    10261 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
    10321 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
    10381 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta
    10441 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
    10501 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga
    10561 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
    10621 gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca
    10681 caaggctact tccctgattg gcagaactac acaccagggc cagggatcag atatccactg
    10741 acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat
    10801 gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag
    10861 agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag
    10921 ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
    10981 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
    11041 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
    11101 gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
    11161 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
    11221 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
    11281 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
    11341 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
    11401 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
    11461 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
    11521 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
    11581 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
    11641 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
    11701 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
    11761 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
    11821 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
    11881 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
    11941 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
    12001 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccagttccgc ccattctccg
    12061 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
    12121 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
    12181 ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat
    12241 agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca
    12301 gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc
    12361 ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg
    12421 tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg
    12481 tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact
    12541 tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt
    12601 tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac
    12661 acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
    12721 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
    12781 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
    12841 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    12901 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
    12961 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
    13021 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
    13081 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
    13141 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
    13201 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    13261 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    13321 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    13381 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    13441 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt c
//
