LOCUS       sequence-388958-        4623 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC27A5_STOP
FEATURES             Location/Qualifiers
     source          1..4623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2180..2279)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2296..2315)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2297..2315)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2320..2336)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2320..2336)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2438..2457)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2449..3258
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2516..2535)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3405..3993
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3894..3913
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4147..4164
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4323..4350)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4442..4528)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4577..4599
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4591..4608
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4592..4608
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggg cgtgaggcag
      121 cagctggccc tgttattact gctgctgctg ctgctgtggg gactgggaca gcccgtgtgg
      181 ccagtggcag tggccctgac cctgaggtgg ctgctgggcg accctacatg ctgcgtgctg
      241 ctgggcctgg ccatgctggc ccgcccatgg ctgggcccct gggtgcctca cggactgagc
      301 ctggcagcag ccgccctggc cctgaccctg ctgccagccc ggctgccccc tggcctgaga
      361 tggctgcccg ccgatgtgat cttcctggcc aagatcctgc acctgggcct gaagatcagg
      421 ggatgcctgt ccaggcagcc accagacaca ttcgtggatg cctttgagag gagagcaagg
      481 gcacagccag gaagagccct gctggtgtgg accggacctg gagcaggatc tgtgacattc
      541 ggcgagctgg acgcaagggc atgccaggca gcatgggccc tgaaggcaga gctgggcgat
      601 cctgcatccc tgtgcgcagg agagccaacc gccctgctgg tgctggcatc tcaggcagtg
      661 cctgccctgt gcatgtggct gggactggca aagctgggat gtccaacagc ctggatcaac
      721 cctcacggaa ggggaatgcc actggcacac agcgtgctga gctccggagc acgggtgctg
      781 gtggtggacc ctgatctgag agagtccctg gaggagatcc tgccaaagct gcaggccgag
      841 aatatcagat gtttctacct gagccacacc tcccctacac caggcgtggg cgccctggga
      901 gccgccctgg acgcagcccc aagccaccca gtgcctgcag atctgagggc aggaatcacc
      961 tggcgctccc ccgccctgtt tatctataca tctggcacca caggcctgcc aaagccagca
     1021 atcctgaccc acgagcgggt gctgcagatg tccaagatgc tgtctctgag cggagcaacc
     1081 gcagacgatg tggtgtacac agtgctgcct ctgtatcacg tgatgggact ggtggtggga
     1141 atcctgggat gcctggacct gggagcaacc tgcgtgctgg caccaaagtt ctccacatct
     1201 tgcttttggg acgattgtag gcagcacggc gtgaccgtga tcctgtacgt gggcgagctg
     1261 ctgcgctatc tgtgcaacat cccacagcag cccgaggaca ggacccacac agtgcgcctg
     1321 gccatgggca atggcctgag agccgacgtg tgggagacct tccagcagcg gtttggcccc
     1381 atcagaatct gggaggtgta cggctctaca gagggcaaca tgggcctggt gaattatgtg
     1441 ggcaggtgcg gcgccctggg caagatgtct tgtctgctgc gcatgctgag ccccttcgag
     1501 ctggtgcagt ttgacatgga ggccgccgag cctgtgaggg ataaccaggg cttctgtatc
     1561 ccagtgggac tgggagagcc tggactgctg ctgaccaagg tggtgtccca gcagccattt
     1621 gtgggctacc ggggccccag agagctgtct gagcgcaagc tggtgaggaa cgtgcgccag
     1681 agcggcgacg tgtactataa tacaggcgac gtgctggcaa tggataggga gggcttcctg
     1741 tactttaggg accgcctggg cgataccttc agatggaagg gcgagaacgt gagcacacac
     1801 gaggtggagg gcgtgctgtc ccaggtggat tttctgcagc aggtgaacgt gtacggcgtg
     1861 tgcgtgccag gatgtgaggg caaggtcgga atggcagcag tgcagctggc acctggacag
     1921 accttcgacg gcgagaagct gtaccagcac gtgcgggcat ggctgcctgc atatgcaaca
     1981 ccacacttta tcagaatcca ggatgccatg gaggtgacct ctacattcaa gctgatgaag
     2041 acccggctgg tgagagaggg ctttaacgtg ggcatcgtgg tggacccact gttcgtgctg
     2101 gataataggg cacagagctt tcgcccactg accgcagaga tgtaccaggc cgtgtgcgag
     2161 ggcacatgga ggctgtaaga cccagctttc ttgtacaaag ttggcattat aagaaagcat
     2221 tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc
     2281 catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
     2341 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     2401 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
     2461 caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
     2521 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag
     2581 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
     2641 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
     2701 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca
     2761 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
     2821 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
     2881 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
     2941 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg
     3001 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
     3061 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     3121 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     3181 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     3241 ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg
     3301 ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg
     3361 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     3421 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3481 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3541 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3601 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3661 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3721 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3781 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3841 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3901 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     3961 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     4021 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     4081 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     4141 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     4201 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     4261 ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg
     4321 caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg
     4381 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
     4441 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
     4501 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg
     4561 ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg
     4621 ccc
//
