LOCUS       sequence-388969-       14307 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR-Cas9 multiplexing acceptor clone with BpiI cutter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14307)
  AUTHORS   Lee RR, Cher WY, Wang J, Chen Y, Chae E
  TITLE     Generating minimum set of gRNA to cover multiple targets in multiple
            genomes with MINORg.
  JOURNAL   Nucleic Acids Res. 2023 Mar 15:gkad142. doi: 10.1093/nar/gkad142.
  PUBMED    36919598
REFERENCE   2  (bases 1 to 14307)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2023 Mar 15:gkad142. doi: 10.1093/nar/gkad142."
COMMENT     Sequence Label: pECNUS_MP01
FEATURES             Location/Qualifiers
     source          1..14307
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1189..1884
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     CDS             complement(2989..3036)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(join(3043..5706,5896..7335))
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system"
                     /label=pcoCas9
                     /note="plant codon-optimized Cas9 gene containing the 
                     potato IV2 intron"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     intron          5707..5895
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             complement(7366..7386)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(7393..7416)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(9145..9468)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     primer_bind     9150..9169
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9363..9385
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9377..9394
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9378..9394
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    9395..9451
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(9464..9480)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9464..9480)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9477..9499)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9488..9504
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9512..9518,9519..9536,9537..9542))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9557..9578
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    9653..9677
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9957..10586
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11015..12088
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      12154..12348
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        12410..12514
                     /gene="bla"
                     /label=AmpR promoter
     CDS             12515..13309
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      13480..14068
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13969..13988
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    14204..14228
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gatccggagt tctagaatgt cgcggaacaa attttaaaac taaatcctaa atttttctaa
       61 ttttgttgcc aatagtggat atgtgggccg tatagaagga atctattgaa ggcccaaacc
      121 catactgacg agcccaaagg ttcgttttgc gttttatgtt tcggttcgat gccaacgcca
      181 cattctgagc taggcaaaaa acaaacgtgt ctttgaatag actcctctcg ttaacacatg
      241 cagcggctgc atggtgacgc cattaacacg tggcctacaa ttgcatgatg tctccattga
      301 cacgtgactt ctcgtctcct ttcttaatat atctaacaaa cactcctacc tcttccaaaa
      361 tatatacaca tctttttgat caatctctca ttcaaaatct cattctctct agtaaacaag
      421 aacaaaaaaa tggcggatac agctagagga acccatcacg atatcatcgg cagagatcag
      481 tacccgatga tgggccgaga tcgtgaccag taccagatgt ccggacgagg atctgactac
      541 tccaagtcta ggcagattgc taaagctgca actgctgtca cagctggtgg ttccctcctt
      601 gttctctcca gccttaccct tgttggaact gtcatagctt tgactgttgc aacacctctg
      661 ctcgttatct tcagcccaat ccttgtcccg gctctcatca cagttgcact cctcatcacc
      721 ggttttcttt cctctggagg gtttggcatt gccgctataa ccgttttctc ttggatttac
      781 aagtaagcac acatttatca tcttacttca taattttgtg caatatgtgc atgcatgtgt
      841 tgagccagta gctttggatc aatttttttg gtcgaataac aaatgtaaca ataagaaatt
      901 gcaaattcta gggaacattt ggttaactaa atacgaaatt tgacctagct agcttgaatg
      961 tgtctgtgta tatcatctat ataggtaaaa tgcttggtat gatacctatt gattgtgaat
     1021 aggtacgcaa cgggagagca cccacaggga tcagacaagt tggacagtgc aaggatgaag
     1081 ttgggaagca aagctcagga tctgaaagac agagctcagt actacggaca gcaacatact
     1141 ggtggggaac atgaccgtga ccgtactcgt ggtggccagc acactactat gagcgagctg
     1201 attaaggaga acatgcacat gaagctgtac atggagggca ccgtgaacaa ccaccacttc
     1261 aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag
     1321 gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctaccag cttcatgtac
     1381 ggcagcagaa ccttcatcaa ccacacccag ggcatccccg acttctttaa gcagtccttc
     1441 cctgagggct tcacatggga gagagtcacc acatacgaag atgggggcgt gctgaccgct
     1501 acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg
     1561 aacttcccat ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccaacacc
     1621 gagatgctgt accccgctga cggcggcctg gaaggcagaa gcgacatggc cctgaagctc
     1681 gtgggcgggg gccacctgat ctgcaacttc aagaccacat acagatccaa gaaacccgct
     1741 aagaacctca agatgcccgg cgtctactat gtggaccaca gactggaaag aatcaaggag
     1801 gccgacaaag aaacctacgt cgagcagcac gaggtggctg tggccagata ctgcgacctc
     1861 cctagcaaac tggggcacaa gtgagcttac cccactgatg tcatcgtcat agtccaataa
     1921 ctccaatgtc ggggagttag tttatgagga ataaagtgtt tagaatttga tcagggggag
     1981 ataataaaag ccgagtttga atctttttgt tataagtaat gtttatgtgt gtttctatat
     2041 gttgtcaaat ggtaccatgt tttttttcct ctctttttgt aacttgcaag tgttgtgttg
     2101 tactttattt ggcttctttg taagttggta acggtggtct atatatggaa aaggtcttgt
     2161 tttgttaaac ttatgttagt taactggatt cgtctttaac cacaaaaagt tttcaataag
     2221 ctacaaattt agacacgcaa gccgatgcag tcattagtac atatatttat tgcaagtgat
     2281 tacatggcaa cccaaacttc aaaaacagta ggttgctcca tttagtcgct gcaagaattc
     2341 aagcttagcg ttgtcaatca attggcaagt cataaaatgc attaaaaaat attttcatac
     2401 tcaactacaa atccatgagt ataactataa ttataaagca atgattagaa tctgacaagg
     2461 attctggaaa attacataaa ggaaagttca taaatgtcta aaacacaaga ggacatactt
     2521 gtattcagta acatttgcag cttttctagg tctgaaaata tatttgttgc ctagtgaata
     2581 agcataatgg tacaactaca agtgttttac tcctcatatt aacttcggtc attagaggcc
     2641 acgatttgac acatttttac tcaaaacaaa atgtttgcat atctcttata atttcaaatt
     2701 caacacacaa caaataagag aaaaaacaaa taatattaat ttgagaatga acaaaaggac
     2761 catatcattc attaactctt ctccatccat ttccatttca cagttcgata gcgaaaaccg
     2821 aataaaaaac acagtaaatt acaagcacaa caaatggtac aagaaaaaca gttttcccaa
     2881 tgccataata ctcaaactca gtaggattct ggtgtgtgcg caatgaaact gatgcattga
     2941 acttgacgaa cgttgtcgaa accgatgata cgaacgaaag caagctcact tcttcttctt
     3001 agcctgtcca gccttcttgg tagcagctgg cctcttgcca ccatctcctc caagctgaga
     3061 aagatcgatt cttgtctcgt aaagtccggt gatagactgg tggataaggg tagcatcaag
     3121 aacctccttg gtagaggtgt atctttttct atcgatggtg gtatcgaagt acttgaaagc
     3181 agctggagca ccaaggttgg taagggtgaa aaggtggatg atgttctcag cctgctctct
     3241 gattggctta tctctgtgct tgttgtaagc agaaagaacc ttatcaaggt tagcatcagc
     3301 aaggataact ctcttagaga actcagagat ttgctcgatg atctcatcaa ggtagtgctt
     3361 gtgctgctca acgaaaagct gcttctgctc gttatcctct ggagatccct tcaacttctc
     3421 gtagtgagaa gcaaggtaaa ggaagttaac gtacttagat ggaagagcaa gctcgtttcc
     3481 cttctgaagc tctccagcag aagcaagcat tctctttctt ccgttctcaa gctcgaaaag
     3541 agagtacttt ggcaacttga tgataagatc cttcttaacc tccttgtatc ccttagcctc
     3601 aaggaaatcg attgggttct tctcgaaaga agaacgctcc atgatggtga ttccaagaag
     3661 ctccttaaca gacttcaact tcttagactt tcccttctca accttagcaa caacaagaac
     3721 agagtaagca acggttggag aatcgaatcc tccgtacttc tttgggtccc aatccttctt
     3781 tctagcgatc aacttatcag agtttctctt tggaaggata gactccttag agaatcctcc
     3841 ggtctgaacc tcggttttct taacgatgtt aacctgtggc atagaaagaa cctttctaac
     3901 ggtagcgaaa tctcttccct tatcccaaac gatctctcct gtctctccgt ttgtctcgat
     3961 aagtggtctt tttctgatct ctccgttagc aagggtgatc tcggtcttga agaagttcat
     4021 gatgttagag tagaagaagt acttagcggt agcctttccg atctcctgct cagacttagc
     4081 gatcatcttt ctaacatcgt aaaccttgta atctccgtaa acgaactcag actccaactt
     4141 tgggtacttc ttgataagag cggttccaac aacagcgtta aggtaagcat cgtgagcgtg
     4201 gtggtagttg ttgatctctc taaccttgta gaactggaaa tcctttctga aatcagaaac
     4261 caacttagac ttcaaggtga taaccttaac ctctctgatc aacttatcgt tctcatcgta
     4321 cttggtgttc atacgagaat caaggatctg agcaacgtgc ttggtgatct gtctggtttc
     4381 aacaagctgt ctcttgatga atccagcctt atcaagctca gaaagtcctc ctctctcagc
     4441 cttggtaagg ttatcgaact ttctctgggt gatcaactta gcgttaagaa gctgtctcca
     4501 gtagttcttc atcttcttaa caacctcctc agatggaacg ttatcagact ttcctctgtt
     4561 cttatcagaa cgggtaagaa ccttgttatc gatagaatca tccttcaaga aagactgtgg
     4621 aacgatgtga tcaacatcgt aatcagaaag tctgttgatg tcaagctcct gatcaacgta
     4681 catatctctt ccgttctgaa ggtagtaaag gtacaacttc tcgttctgaa gctgggtgtt
     4741 ctcaactggg tgctccttca agatttgaga tccaagctcc ttgattccct cctcgattct
     4801 cttcattctc tcacgagagt tcttctgtcc cttctgggtg gtctggttct ctctagccat
     4861 ctcgataacg atgttctctg gcttgtgtct acccataacc ttaacaagct catcaacaac
     4921 cttaacggtc tgaaggattc ccttcttgat agctggagat ccagcaaggt tagcgatgtg
     4981 ctcgtgaaga gaatctccct gtccagaaac ctgagccttc tggatgtcct ccttgaaggt
     5041 aagagaatca tcgtggataa gctgcatgaa gtttctgtta gcgaatccat cagacttcaa
     5101 gaaatcaagg atggtctttc cagactgctt atctctgatt ccgttgatca acttacgaga
     5161 aagtctaccc catccggtgt atcttcttct cttcaactgc ttcataacct tatcatcgaa
     5221 aaggtgagcg taggtcttga gtctctcctc gatcatctct ctatcctcga aaagggtaag
     5281 ggtaagaacg atgtcctcaa ggatgtcctc gttctcctcg ttatcaagga aatccttatc
     5341 cttgatgatc ttcaaaagat cgtggtaggt tccaagagaa gcgttgaatc tatcctcaac
     5401 tccagagatt tcaacagaat cgaagcactc gatcttcttg aagtaatcct ccttcaactg
     5461 cttaacggta acctttctgt tggtcttgaa aagaagatca acgatagcct tcttctgctc
     5521 tccagaaagg aaagctggct ttctcattcc ctcggtaacg tacttaacct tggtaagctc
     5581 gttgtaaacg gtgaagtact cgtaaagaag agagtgcttt ggaagaacct tctcgtttgg
     5641 aaggttctta tcgaagttgg tcattctctc gatgaaagac tgagcagaag ctcccttatc
     5701 aacaacctgc acatcaacaa attttggtca tatattagaa aagttataaa ttaaaatata
     5761 cacacttata aactacagaa aagcaattgc tatatactac attcttttat tttgaaaaaa
     5821 atatttgaaa tattatatta ctactaatta atgataatta ttatatatat atcaaaggta
     5881 gaagcagaaa cttacctcct cgaagttcca aggggtgatt gtctcctcag actttctggt
     5941 catccaagcg aaacgagagt ttcctctagc aagtggtcca acgtagtatg ggattctgaa
     6001 ggtaaggatc ttctcgatct tctctctgtt atccttcaag aatgggtaga aatcctcctg
     6061 tctacgaagg atagcgtgaa gctctccaag gtggatctgg tgtgggatag atccgttatc
     6121 gaaggttctc tgctttctaa gaagatcctc tctgttcaac ttaacaagaa gctcctcggt
     6181 tccatccatc ttctcaagga ttggcttgat gaacttgtag aactcctcct gagaagctcc
     6241 tccatcgatg tatccagcgt atccgttctt agactgatcg aagaagattt ccttgtactt
     6301 ctctggaagc tgctgtctaa caagagcctt caaaagggta agatcctggt ggtgctcatc
     6361 gtatctcttg atcatagaag cagaaagtgg agccttggtg atctcggtgt taactctaag
     6421 gatgtcagaa agaaggatag catcagaaag gttcttagca gcaaggaaaa gatcagcgta
     6481 ctgatctccg atctgagcaa gaaggttatc aagatcatca tcgtaggtat ccttagaaag
     6541 ctgcaactta gcatcctcag caagatcgaa gttagacttg aagtttgggg taagtccaag
     6601 agaaagagcg ataaggtttc cgaaaagtcc gttcttcttc tctcctggaa gctgagcgat
     6661 aaggttctca agtctacgag acttagaaag tctagcagaa aggatagcct tagcatcaac
     6721 tccagaagcg ttgattgggt tctcctcgaa aagctggttg taggtctgaa caagctggat
     6781 gaacaactta tcaacatcag agttatctgg gttaagatct ccctcgataa ggaagtgtcc
     6841 tctgaacttg atcatgtgag caagagcaag gtagataagt ctaagatcag ccttatcggt
     6901 agaatcaacc aacttctttc taaggtggta gatggttggg tacttctcgt ggtaagcaac
     6961 ctcatcaacg atgtttccga agattgggtg tctctcgtgc ttcttatcct cctcaacaag
     7021 gaaagactcc tcaagtctgt ggaagaaaga atcatcaacc ttagccatct cgttagagaa
     7081 gatttcctga aggtagcaga ttctgttctt tcttctggtg tatcttcttc tagcggttct
     7141 cttcaatctg gtagcctcag cggtttctcc agaatcgaaa agaagagcac cgataaggtt
     7201 cttcttgata gagtgtctat cggtgtttcc aagaaccttg aacttcttag atggaacctt
     7261 gtactcatcg gtgataacag cccatccaac agagttggtt ccgatgtcaa gtccgataga
     7321 gtacttctta tccatacctc cagcagctgg aactccgtgg attccaacct ttctcttctt
     7381 ctttggagcc atcttatcat catcatcctt gtaatccatt ggctgtggtg agagaaacag
     7441 agcgtgagct caaatacaca agtacgaaat cagagttatg agagaggatt tggagaaaga
     7501 agatcacctg aagatcgttt gcgtcgcgcg aatgagagag acggcagaga aaacgagaag
     7561 ctttaagagg acgaacaaaa accctaatgt cactcctttt atatagatga gaaatttagg
     7621 gtttgggctc taatggagct gtctacatgg gctttcattt ctttatccag taaagaaccg
     7681 gtttaacagg ttccctggtt tatacgtggt tttagccggt taacttacgg aatattattt
     7741 ttatcttaag ttacacaaat tacatcatta atagtaaaac aaaattttat ggtgcaattt
     7801 aagtttttat gatgagtgaa attcgaataa tatacaaata ttttactgtt taggtttccc
     7861 taaacctact tttagatatg taaattaaac attttttttt ttttggttag aaccaagtca
     7921 gtgatcatag aactcaaaaa gctggtgttt caattggtac aagcattttt attgcaagat
     7981 ataaaaattc agaaggacat atccttgatg tatgtacatg aaggaaccga ttttgactcc
     8041 aagctacaaa gaagaagaag aatgatatac aaagaaaact gcatatcctc accatgaaat
     8101 tgaaagctag aggaagggga ataacagata ctctgcgtag aagagattcc atataaaccg
     8161 ataatatcct gagatagctt cctgcaataa tcaaatgttt ctacactccg ttagatatac
     8221 actccattga tgttccgtga taacgagttt atgtttgtac aaaagtattt taccttggtg
     8281 tagtttgctt tttctagtac ccatgtctac caaagatcaa caacaaagat attagagatt
     8341 aatgaatttg gcagtgtcaa atgcagaaac taaagcatat agttatgaag ttcttgtgca
     8401 ttatctcaaa agtatgtaag agatataaga gtatctcata atgctttacc tggaaaatta
     8461 agcctgaagc caagatcaca tgtgcaggaa tcatcaagct acctctaaaa acctgttcat
     8521 tgttaaagga taagagggtt ggtcttttca aagctgcata ctgaggaaag taacaatatc
     8581 gcaacaaaaa taaattggtg gcgatagttt atcaagcttc tacatgcagt aatggtgttc
     8641 gtgctttaat taaccgaaat agttaagacg gaaatagatg aattcacctg aggcatgtag
     8701 aaagctagtg atatggctga aacataattt actagcagaa gtccagaacc gaggaatgca
     8761 atgtttctca ctccaagttt tgttgccagg gttgatattt ggaacctatt caagatcaat
     8821 caaaggcatg attaactaaa catgcttgaa caaacacata aagacaatat atcaaacgga
     8881 gctggttaaa cattgtcttt gactgtcaaa tggattgagc caaagagcgg aaagaaaatg
     8941 gttatgcgtg cagttactta ctttcgatct ccttcaacat caggaaggtc ctttgtaata
     9001 gcaatgacca gtgcaaacag tgtcacaaaa gatgtgatga acgccacagg tgcactgcaa
     9061 atagcgaatc aaaagttgag taatatatct actcacgatt atggggagct ccactagaat
     9121 tcgagctcgg agtgccaagt cttcctatgc ggcatcagag cagattgtac tgagagtgca
     9181 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
     9241 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     9301 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     9361 ttcccagtca cgacgttgta aaacgacggc cagtgaattc gagctcggta cccggggatc
     9421 ctctagagtc gacctgcagg catgcaagct tggcgtaatc atggtcatag ctgtttcctg
     9481 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     9541 aagcctgggg tgcctaatga gtgagctaac tcacattaga agacaaggga gctttacgag
     9601 gatgcacatg tgaccgaggg acacgaagtg atccgtttaa actatcagtg tttgacagga
     9661 tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa
     9721 gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc cagccgtgcg gctgcatgaa
     9781 atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa
     9841 gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat
     9901 gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga
     9961 aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc
    10021 tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg
    10081 gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg
    10141 accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg
    10201 acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc
    10261 tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg
    10321 ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg
    10381 cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc
    10441 ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca
    10501 caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg
    10561 ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac
    10621 aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag
    10681 cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac
    10741 caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata
    10801 catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg
    10861 ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca
    10921 tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca
    10981 atggcactgg aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc
    11041 ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc
    11101 cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc
    11161 tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgtcgt cgattaggaa
    11221 gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac
    11281 ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg
    11341 agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc
    11401 ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac
    11461 cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc
    11521 acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga
    11581 cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa
    11641 ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa
    11701 gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat
    11761 gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt
    11821 tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa
    11881 ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt
    11941 caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga
    12001 tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat
    12061 cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct
    12121 agcaggggaa aaaggtcgaa aaagcttctt tcctgtggat agcacgtaca ttgggaaccc
    12181 aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa
    12241 ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa
    12301 ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca
    12361 gcgcacagcc gaacagctgc aaaaagcgcc cacatcaagg ctccgagtgc gcggaacccc
    12421 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
    12481 ataaatgctt caataatatt gaaaaaggaa gagtatggct aaaatgagaa tatcaccgga
    12541 attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc
    12601 taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg
    12661 gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg
    12721 aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct
    12781 catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga
    12841 aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga
    12901 ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa
    12961 cgatctggcc gatgtggatt gcgaaaactg ggaagaggac actccattta aagatccgcg
    13021 cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg
    13081 cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct
    13141 tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgctcag
    13201 ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc
    13261 tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagc tgtcagacca
    13321 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
    13381 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
    13441 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
    13501 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
    13561 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
    13621 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
    13681 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
    13741 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
    13801 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
    13861 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
    13921 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
    13981 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
    14041 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
    14101 gctcggatct gttggaccgg acagtagtca tggttgatgg gctgcctgta tcgagtggtg
    14161 attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg
    14221 gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact
    14281 ggggttgaac actctgtgcc gaattcg
//
