LOCUS       sequence-38908-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Sander JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung JK, Yeh JR
  TITLE     Targeted gene disruption in somatic zebrafish cells using engineered
            TALENs.
  JOURNAL   Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934.
  PUBMED    21822241
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2011-08-5- 5"; volume: "29"; 
            issue: "8"; pages: "697-8"
COMMENT     Sequence Label: TAL020
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(189..206)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(189..205)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(198..220)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(414..433)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     533..555
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(593..611)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        679..783
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcaggcctct gcagtcgacg ggcccgggat ccggtctctt caaaccgtgg gcttgacaca
       61 acacgggaag gagccgttgg acagtctcca atgcttgttt ccctccaccg ttggacgcaa
      121 tcgctacaac ttgatcggga gtcagttgtc ttctctagat gcattcgcga ggtaccgagc
      181 tcgaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      241 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      301 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
      361 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
      421 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
      481 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
      541 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
      601 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
      661 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      721 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      781 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
      841 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaa
//
