LOCUS       sequence-389270-       10968 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encoding human ErbB3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10968)
  AUTHORS   Matsuda K, Hirayama D, Hino N, Kuno S, Sakaue-Sawano A, Miyawaki A, 
            Matsuda M, Terai K
  TITLE     Knockout of all ErbB-family genes delineates their roles in 
            proliferation, survival, and migration.
  JOURNAL   J Cell Sci. 2023 Jul 31:jcs.261199. doi: 10.1242/jcs.261199.
  PUBMED    37519219
REFERENCE   2  (bases 1 to 10968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2023 Jul 31:jcs.261199. doi: 10.1242/jcs.261199."
COMMENT     Sequence Label: pPBpuro-ErbB3
FEATURES             Location/Qualifiers
     source          1..10968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1392..1667
                     /label=chicken beta-actin promoter
     intron          1668..2684
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2608..2630
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2692..2711
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     misc_feature    6874..7423
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7018..7035)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7245..7264
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7426..8025
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7426..7445)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7922..7942
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(8052..8069)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8058..8282
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8329..8345)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8353..8369
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8377..8383,8384..8401,8402..8407))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8422..8443
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   8677..8739
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8780..8800)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8780..8798)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8819..8835)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8843..8859
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8867..8873,8874..8891,8892..8897))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8912..8933
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9050..9067)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9221..9809)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9301..9320)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9980..10771,10772..10840))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10603..10622
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10841..10945)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     1441 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     1501 gggggggggg gsgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc
     1561 ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga
     1621 ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc
     1681 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
     1741 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
     1801 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
     1861 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
     1921 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
     1981 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
     2041 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
     2101 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
     2161 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
     2221 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
     2281 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
     2341 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     2401 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     2461 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     2521 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     2581 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     2641 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     2701 tggttattgt gctgtctcat cattttggca aagaattggg atccgaattc ctcgagggcg
     2761 gccgccacca tgagggcgaa cgacgctctg caggtgctgg gcttgctttt cagcctggcc
     2821 cggggctccg aggtgggcaa ctctcaggca gtgtgtcctg ggactctgaa tggcctgagt
     2881 gtgaccggcg atgctgagaa ccaataccag acactgtaca agctctacga gaggtgtgag
     2941 gtggtgatgg ggaaccttga gattgtgctc acgggacaca atgccgacct ctccttcctg
     3001 cagtggattc gagaagtgac aggctatgtc ctcgtggcca tgaatgaatt ctctactcta
     3061 ccattgccca acctccgcgt ggtgcgaggg acccaggtct acgatgggaa gtttgccatc
     3121 ttcgtcatgt tgaactataa caccaactcc agccacgctc tgcgccagct ccgcttgact
     3181 cagctcaccg agattctgtc agggggtgtt tatattgaga agaacgataa gctttgtcac
     3241 atggacacaa ttgactggag ggacatcgtg agggaccgag atgctgagat agtggtgaag
     3301 gacaatggca gaagctgtcc cccctgtcat gaggtttgca aggggcgatg ctggggtcct
     3361 ggatcagaag actgccagac attgaccaag accatctgtg ctcctcagtg taatggtcac
     3421 tgctttgggc ccaaccccaa ccagtgctgc catgatgagt gtgccggggg ctgctcaggc
     3481 cctcaggaca cagactgctt tgcctgccgg cacttcaatg acagtggagc ctgtgtacct
     3541 cgctgtccac agcctcttgt ctacaacaag ctaactttcc agctggaacc caatccccac
     3601 accaagtatc agtatggagg agtttgtgta gccagctgtc cccataactt tgtggtggat
     3661 caaacatcct gtgtcagggc ctgtcctcct gacaagatgg aagtagataa aaatgggctc
     3721 aagatgtgtg agccttgtgg gggactatgt cccaaagcct gtgagggaac aggctctggg
     3781 agccgcttcc agactgtgga ctcgagcaac attgatggat ttgtgaactg caccaagatc
     3841 ctgggcaacc tggactttct gatcaccggc ctcaatggag acccctggca caagatccct
     3901 gccctggacc cagagaagct caatgtcttc cggacagtac gggagatcac aggttacctg
     3961 aacatccagt cctggccgcc ccacatgcac aacttcagtg ttttttccaa tttgacaacc
     4021 attggaggca gaagcctcta caaccggggc ttctcattgt tgatcatgaa gaacttgaat
     4081 gtcacatctc tgggcttccg atccctgaag gaaattagtg ctgggcgtat ctatataagt
     4141 gccaataggc agctctgcta ccaccactct ttgaactgga ccaaggtgct tcgggggcct
     4201 acggaagagc gactagacat caagcataat cggccgcgca gagactgcgt ggcagagggc
     4261 aaagtgtgtg acccactgtg ctcctctggg ggatgctggg gcccaggccc tggtcagtgc
     4321 ttgtcctgtc gaaattatag ccgaggaggt gtctgtgtga cccactgcaa ctttctgaat
     4381 ggggagcctc gagaatttgc ccatgaggcc gaatgcttct cctgccaccc ggaatgccaa
     4441 cccatggagg gcactgccac atgcaatggc tcgggctctg atacttgtgc tcaatgtgcc
     4501 cattttcgag atgggcccca ctgtgtgagc agctgccccc atggagtcct aggtgccaag
     4561 ggcccaatct acaagtaccc agatgttcag aatgaatgtc ggccctgcca tgagaactgc
     4621 acccaggggt gtaaaggacc agagcttcaa gactgtttag gacaaacact ggtgctgatc
     4681 ggcaaaaccc atctgacaat ggctttgaca gtgatagcag gattggtagt gattttcatg
     4741 atgctgggcg gcacttttct ctactggcgt gggcgccgga ttcagaataa aagggctatg
     4801 aggcgatact tggaacgggg tgagagcata gagcctctgg accccagtga gaaggctaac
     4861 aaagtcttgg ccagaatctt caaagagaca gagctaagga agcttaaagt gcttggctcg
     4921 ggtgtctttg gaactgtgca caaaggagtg tggatccctg agggtgaatc aatcaagatt
     4981 ccagtctgca ttaaagtcat tgaggacaag agtggacggc agagttttca agctgtgaca
     5041 gatcatatgc tggccattgg cagcctggac catgcccaca ttgtaaggct gctgggacta
     5101 tgcccagggt catctctgca gcttgtcact caatatttgc ctctgggttc tctgctggat
     5161 catgtgagac aacaccgggg ggcactgggg ccacagctgc tgctcaactg gggagtacaa
     5221 attgccaagg gaatgtacta ccttgaggaa catggtatgg tgcatagaaa cctggctgcc
     5281 cgaaacgtgc tactcaagtc acccagtcag gttcaggtgg cagattttgg tgtggctgac
     5341 ctgctgcctc ctgatgataa gcagctgcta tacagtgagg ccaagactcc aattaagtgg
     5401 atggcccttg agagtatcca ctttgggaaa tacacacacc agagtgatgt ctggagctat
     5461 ggtgtgacag tttgggagtt gatgaccttc ggggcagagc cctatgcagg gctacgattg
     5521 gctgaagtac cagacctgct agagaagggg gagcggttgg cacagcccca gatctgcaca
     5581 attgatgtct acatggtgat ggtcaagtgt tggatgattg atgagaacat tcgcccaacc
     5641 tttaaagaac tagccaatga gttcaccagg atggcccgag acccaccacg gtatctggtc
     5701 ataaagagag agagtgggcc tggaatagcc cctgggccag agccccatgg tctgacaaac
     5761 aagaagctag aggaagtaga gctggagcca gaactagacc tagacctaga cttggaagca
     5821 gaggaggaca acctggcaac caccacactg ggctccgccc tcagcctacc agttggaaca
     5881 cttaatcggc cacgtgggag ccagagcctt ttaagtccat catctggata catgcccatg
     5941 aaccagggta atcttgggga gtcttgccag gagtctgcag tttctgggag cagtgaacgg
     6001 tgcccccgtc cagtctctct acacccaatg ccacggggat gcctggcatc agagtcatca
     6061 gaggggcatg taacaggctc tgaggctgag ctccaggaga aagtgtcaat gtgtaggagc
     6121 cggagcagga gccggagccc acggccacgc ggagatagcg cctaccattc ccagcgccac
     6181 agtctgctga ctcctgttac cccactctcc ccacccgggt tagaggaaga ggatgtcaac
     6241 ggttatgtca tgccagatac acacctcaaa ggtactccct cctcccggga aggcaccctt
     6301 tcttcagtgg gtctcagttc tgtcctgggt actgaagaag aagatgaaga tgaggagtat
     6361 gaatacatga accggaggag aaggcacagt ccacctcatc cccctaggcc aagttccctt
     6421 gaggagctgg gttatgagta catggatgtg gggtcagacc tcagtgcctc tctgggcagc
     6481 acacagagtt gcccactcca ccctgtaccc atcatgccca ctgcaggcac aactccagat
     6541 gaagactatg aatatatgaa tcggcaacga gatggaggtg gtcctggggg tgattatgca
     6601 gccatggggg cctgcccagc atctgagcaa gggtatgaag agatgagagc ttttcagggg
     6661 cctggacatc aggcccccca tgtccattat gcccgcctaa aaactctacg tagcttagag
     6721 gctacagact ctgcctttga taaccctgat tactggcata gcaggctttt ccccaaggct
     6781 aatgcccaga gaacgtaagg ccgctctaga gtcaactaac ttaagctagc aacggtttcc
     6841 ctctagcggg atcaattccg cccccccccc ctaacgttac tggccgaagc cgcttggaat
     6901 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     6961 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     7021 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     7081 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     7141 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     7201 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     7261 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     7321 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     7381 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgata ataccatgac cgagtacaag
     7441 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     7501 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     7561 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     7621 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     7681 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     7741 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     7801 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     7861 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     7921 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     7981 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgatcgat gatctagagc
     8041 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     8101 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     8161 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     8221 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     8281 ggcttctgag gcggaaagaa cctgcagccc aagcttggcg taatcatggt catagctgtt
     8341 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     8401 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     8461 gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc ttaaaagttt tgttacttta
     8521 tagaagaaat tttgagtttt tgtttttttt taataaataa ataaacataa ataaattgtt
     8581 tgttgaattt attattagta tgtaagtgta aatataataa aacttaatat ctattcaaat
     8641 taataaataa acctcgatat acagaccgat aaaacacatg cgtcaatttt acgcatgatt
     8701 atctttaacg tacgtcacaa tatgattatc tttctagggt taatctagta tacgcgttta
     8761 attaatccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt
     8821 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     8881 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     8941 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     9001 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     9061 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     9121 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     9181 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     9241 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     9301 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     9361 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     9421 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     9481 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     9541 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     9601 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     9661 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     9721 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     9781 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     9841 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     9901 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     9961 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    10021 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    10081 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    10141 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    10201 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    10261 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    10321 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    10381 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    10441 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    10501 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    10561 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    10621 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    10681 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    10741 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    10801 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    10861 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    10921 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
