LOCUS       sequence-389271-       10866 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encoding human ErbB4..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10866)
  AUTHORS   Matsuda K, Hirayama D, Hino N, Kuno S, Sakaue-Sawano A, Miyawaki A, 
            Matsuda M, Terai K
  TITLE     Knockout of all ErbB-family genes delineates their roles in 
            proliferation, survival, and migration.
  JOURNAL   J Cell Sci. 2023 Jul 31:jcs.261199. doi: 10.1242/jcs.261199.
  PUBMED    37519219
REFERENCE   2  (bases 1 to 10866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2023 Jul 31:jcs.261199. doi: 10.1242/jcs.261199."
COMMENT     Sequence Label: pPBpuro-ErbB4
FEATURES             Location/Qualifiers
     source          1..10866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1392..1667
                     /label=chicken beta-actin promoter
     intron          1668..2684
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2608..2630
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2692..2711
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     misc_feature    6772..7321
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6916..6933)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7143..7162
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7324..7923
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7324..7343)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7820..7840
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7950..7967)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7956..8180
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8227..8243)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8251..8267
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8275..8281,8282..8299,8300..8305))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8320..8341
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   8575..8637
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8678..8698)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8678..8696)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8717..8733)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8741..8757
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8765..8771,8772..8789,8790..8795))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8810..8831
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8948..8965)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9119..9707)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9199..9218)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9878..10669,10670..10738))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10501..10520
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10739..10843)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     1441 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     1501 gggggggggg gsgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc
     1561 ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga
     1621 ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc
     1681 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
     1741 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
     1801 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
     1861 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
     1921 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
     1981 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
     2041 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
     2101 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
     2161 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
     2221 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
     2281 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
     2341 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     2401 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     2461 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     2521 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     2581 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     2641 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     2701 tggttattgt gctgtctcat cattttggca aagaattggg atccgaattc ctcgagggcg
     2761 gccgccacca tgaagccggc gacaggactt tgggtctggg tgagccttct cgtggcggcg
     2821 gggaccgtcc agcccagcga ttctcagtca gtgtgtgcag gaacggagaa taaactgagc
     2881 tctctctctg acctggaaca gcagtaccga gccttgcgca agtactatga aaactgtgag
     2941 gttgtcatgg gcaacctgga gataaccagc attgagcaca accgggacct ctccttcctg
     3001 cggtctgttc gagaagtcac aggctacgtg ttagtggctc ttaatcagtt tcgttacctg
     3061 cctctggaga atttacgcat tattcgtggg acaaaacttt atgaggatcg atatgccttg
     3121 gcaatatttt taaactacag aaaagatgga aactttggac ttcaagaact tggattaaag
     3181 aacttgacag aaatcctaaa tggtggagtc tatgtagacc agaacaaatt cctttgttat
     3241 gcagacacca ttcattggca agatattgtt cggaacccat ggccttccaa cttgactctt
     3301 gtgtcaacaa atggtagttc aggatgtgga cgttgccata agtcctgtac tggccgttgc
     3361 tggggaccca cagaaaatca ttgccagact ttgacaagga cggtgtgtgc agaacaatgt
     3421 gacggcagat gctacggacc ttacgtcagt gactgctgcc atcgagaatg tgctggaggc
     3481 tgctcaggac ctaaggacac agactgcttt gcctgcatga atttcaatga cagtggagca
     3541 tgtgttactc agtgtcccca aacctttgtc tacaatccaa ccacctttca actggagcac
     3601 aatttcaatg caaagtacac atatggagca ttctgtgtca agaaatgtcc acataacttt
     3661 gtggtagatt ccagttcttg tgtgcgtgcc tgccctagtt ccaagatgga agtagaagaa
     3721 aatgggatta aaatgtgtaa accttgcact gacatttgcc caaaagcttg tgatggcatt
     3781 ggcacaggat cattgatgtc agctcagact gtggattcca gtaacattga caaattcata
     3841 aactgtacca agatcaatgg gaatttgatc tttctagtca ctggtattca tggggaccct
     3901 tacaatgcaa ttgaagccat agacccagag aaactgaacg tctttcggac agtcagagag
     3961 ataacaggtt tcctgaacat acagtcatgg ccaccaaaca tgactgactt cagtgttttt
     4021 tctaacctgg tgaccattgg tggaagagta ctctatagtg gcctgtcctt gcttatcctc
     4081 aagcaacagg gcatcacctc tctacagttc cagtccctga aggaaatcag cgcaggaaac
     4141 atctatatta ctgacaacag caacctgtgt tattatcata ccattaactg gacaacactc
     4201 ttcagcacaa tcaaccagag aatagtaatc cgggacaaca gaaaagctga aaattgtact
     4261 gctgaaggaa tggtgtgcaa ccatctgtgt tccagtgatg gctgttgggg acctgggcca
     4321 gaccaatgtc tgtcgtgtcg ccgcttcagt agaggaagga tctgcataga gtcttgtaac
     4381 ctctatgatg gtgaatttcg ggagtttgag aatggctcca tctgtgtgga gtgtgacccc
     4441 cagtgtgaga agatggaaga tggcctcctc acatgccatg gaccgggtcc tgacaactgt
     4501 acaaagtgct ctcattttaa agatggccca aactgtgtgg aaaaatgtcc agatggctta
     4561 cagggggcaa acagtttcat tttcaagtat gctgatccag atcgggagtg ccacccatgc
     4621 catccaaact gcacccaagg gtgtaacggt cccactagtc atgactgcat ttactaccca
     4681 tggacgggcc attccacttt accacaacat gctagaactc ccctgattgc agctggagta
     4741 attggtgggc tcttcattct ggtcattgtg ggtctgacat ttgctgttta tgttagaagg
     4801 aagagcatca aaaagaaaag agccttgaga agattcttgg aaacagagtt ggtggaacca
     4861 ttaactccca gtggcacagc acccaatcaa gctcaacttc gtattttgaa agaaactgag
     4921 ctgaagaggg taaaagtcct tggctcaggt gcttttggaa cggtttataa aggtatttgg
     4981 gtacctgaag gagaaactgt gaagattcct gtggctatta agattcttaa tgagacaact
     5041 ggtcccaagg caaatgtgga gttcatggat gaagctctga tcatggcaag tatggatcat
     5101 ccacacctag tccggttgct gggtgtgtgt ctgagcccaa ccatccagct ggttactcaa
     5161 cttatgcccc atggctgcct gttggagtat gtccacgagc acaaggataa cattggatca
     5221 caactgctgc ttaactggtg tgtccagata gctaagggaa tgatgtacct ggaagaaaga
     5281 cgactcgttc atcgggattt ggcagcccgt aatgtcttag tgaaatctcc aaaccatgtg
     5341 aaaatcacag attttgggct agccagactc ttggaaggag atgaaaaaga gtacaatgct
     5401 gatggaggaa agatgccaat taaatggatg gctctggagt gtatacatta caggaaattc
     5461 acccatcaga gtgacgtttg gagctatgga gttactatat gggaactgat gacctttgga
     5521 ggaaaaccct atgatggaat tccaacgcga gaaatccctg atttattaga gaaaggagaa
     5581 cgtttgcctc agcctcccat ctgcactatt gacgtttaca tggtcatggt caaatgttgg
     5641 atgattgatg ctgacagtag acctaaattt aaggaactgg ctgctgagtt ttcaaggatg
     5701 gctcgagacc ctcaaagata cctagttatt cagggtgatg atcgtatgaa gcttcccagt
     5761 ccaaatgaca gcaagttctt tcagaatctc ttggatgaag aggatttgga agatatgatg
     5821 gatgctgagg agtacttggt ccctcaggct ttcaacatcc cacctcccat ctatacttcc
     5881 agagcaagaa ttgactcgaa taggagtgaa attggacaca gccctcctcc tgcctacacc
     5941 cccatgtcag gaaaccagtt tgtataccga gatggaggtt ttgctgctga acaaggagtg
     6001 tctgtgccct acagagcccc aactagcaca attccagaag ctcctgtggc acagagtgct
     6061 actgctgaga tttttgatga ctcctgctgt aatggcaccc tacgcaagcc agtggcaccc
     6121 catgtccaag aggacagtag cacccagagg tacagtgctg accccaccgt gtttgcccca
     6181 gaacggagcc cacgaggaga gctggatgag gaaggttaca tgactcctat gcgagacaaa
     6241 cccaaacaag aatacctgaa tccagtggag gagaaccctt ttgtttctcg gagaaaaaat
     6301 ggagaccttc aagcattgga taatcccgaa tatcacaatg catccaatgg tccacccaag
     6361 gccgaggatg agtatgtgaa tgagccactg tacctcaaca cctttgccaa caccttggga
     6421 aaagctgagt acctgaagaa caacatactg tcaatgccag agaaggccaa gaaagcgttt
     6481 gacaaccctg actactggaa ccacagcctg ccacctcgga gcacccttca gcacccagac
     6541 tacctgcagg agtacagcac aaaatatttt tataaacaga atgggcggat ccggcctatt
     6601 gtggcagaga atcctgaata cctctctgag ttctccctga agccaggcac tgtgctgccg
     6661 cctccacctt acagacaccg gaatactgtg gtgtaaggcc gctctagagt caactaactt
     6721 aagctagcaa cggtttccct ctagcgggat caattccgcc ccccccccct aacgttactg
     6781 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     6841 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     6901 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
     6961 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
     7021 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
     7081 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     7141 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
     7201 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa
     7261 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgataat
     7321 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     7381 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     7441 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     7501 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     7561 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     7621 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     7681 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     7741 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     7801 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     7861 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     7921 tgatcgatga tctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     7981 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     8041 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     8101 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     8161 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc tgcagcccaa gcttggcgta
     8221 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     8281 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     8341 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatctt
     8401 aaaagttttg ttactttata gaagaaattt tgagtttttg ttttttttta ataaataaat
     8461 aaacataaat aaattgtttg ttgaatttat tattagtatg taagtgtaaa tataataaaa
     8521 cttaatatct attcaaatta ataaataaac ctcgatatac agaccgataa aacacatgcg
     8581 tcaattttac gcatgattat ctttaacgta cgtcacaata tgattatctt tctagggtta
     8641 atctagtata cgcgtttaat taatccagct tttgttccct ttagtgaggg ttaattgcgc
     8701 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     8761 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     8821 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     8881 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     8941 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     9001 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     9061 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     9121 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     9181 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     9241 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     9301 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     9361 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     9421 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     9481 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     9541 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     9601 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     9661 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     9721 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     9781 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     9841 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     9901 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     9961 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    10021 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    10081 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    10141 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    10201 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    10261 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    10321 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    10381 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    10441 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    10501 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    10561 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    10621 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    10681 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    10741 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    10801 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    10861 tgccac
//
