LOCUS       sequence-389606-       10966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encoding human ErbB1 tagged with mScarlet..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10966)
  AUTHORS   Matsuda K, Hirayama D, Hino N, Kuno S, Sakaue-Sawano A, Miyawaki A, 
            Matsuda M, Terai K
  TITLE     Knockout of all ErbB-family genes delineates their roles in 
            proliferation, survival, and migration.
  JOURNAL   J Cell Sci. 2023 Jul 31:jcs.261199. doi: 10.1242/jcs.261199.
  PUBMED    37519219
REFERENCE   2  (bases 1 to 10966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2023 Jul 31:jcs.261199. doi: 10.1242/jcs.261199."
COMMENT     Sequence Label: pKUNOC-ErbB1-mScarlet
FEATURES             Location/Qualifiers
     source          1..10966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        123..502
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        504..702
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             720..900
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    947..1072
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1569..1802
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1987..2031
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2305..2422
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2523..2826
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2827..3030
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             join(3082..3153,3154..5016,5017..5085,5086..6711)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     CDS             6712..7407
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(6845..6863)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7113..7132
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    7460..8048
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7513..7533)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7931..7942)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8567..8747
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8773..8790)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8779..9003
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(9099..9687)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9179..9198)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9858..10649,10650..10718))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10719..10823)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10898..10917)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
       61 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
      121 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
      181 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      241 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      301 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      361 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      421 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      481 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
      541 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
      601 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
      661 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg
      721 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
      781 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
      841 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
      901 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
      961 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     1021 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     1081 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     1141 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     1201 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     1261 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     1321 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     1381 aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga
     1441 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1501 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1561 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1621 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     1681 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     1741 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     1801 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     1861 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     1921 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     1981 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     2041 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     2101 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     2161 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     2221 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
     2281 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2341 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2401 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagaaatt cactttgaat
     2461 taattcgatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     2521 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     2581 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     2641 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     2701 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     2761 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     2821 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     2881 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     2941 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     3001 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc
     3061 taccggtctc gagaattcac catgcgaccc tccgggacgg ccggggcagc gctcctggcg
     3121 ctgctggctg cgctctgccc ggcgagtcgg gctctggagg aaaagaaagt ttgccaaggc
     3181 acgagtaaca agctcacgca gttgggcact tttgaagatc attttctcag cctccagagg
     3241 atgttcaata actgtgaggt ggtccttggg aatttggaaa ttacctatgt gcagaggaat
     3301 tatgatcttt ccttcttaaa gaccatccag gaggtggctg gttatgtcct cattgccctc
     3361 aacacagtgg agcgaattcc tttggaaaac ctgcagatca tcagaggaaa tatgtactac
     3421 gaaaattcct atgccttagc agtcttatct aactatgatg caaataaaac cggactgaag
     3481 gagctgccca tgagaaattt acaggaaatc ctgcatggcg ccgtgcggtt cagcaacaac
     3541 cctgccctgt gcaacgtgga gagcatccag tggcgggaca tagtcagcag tgactttctc
     3601 agcaacatgt cgatggactt ccagaaccac ctgggcagct gccaaaagtg tgatccaagc
     3661 tgtcccaatg ggagctgctg gggtgcagga gaggagaact gccagaaact gaccaaaatc
     3721 atctgtgccc agcagtgctc cgggcgctgc cgtggcaagt cccccagtga ctgctgccac
     3781 aaccagtgtg ctgcaggctg cacaggcccc cgggagagcg actgcctggt ctgccgcaaa
     3841 ttccgagacg aagccacgtg caaggacacc tgccccccac tcatgctcta caaccccacc
     3901 acgtaccaga tggatgtgaa ccccgagggc aaatacagct ttggtgccac ctgcgtgaag
     3961 aagtgtcccc gtaattatgt ggtgacagat cacggctcgt gcgtccgagc ctgtggggcc
     4021 gacagctatg agatggagga agacggcgtc cgcaagtgta agaagtgcga agggccttgc
     4081 cgcaaagtgt gtaacggaat aggtattggt gaatttaaag actcactctc cataaatgct
     4141 acgaatatta aacacttcaa aaactgcacc tccatcagtg gcgatctcca catcctgccg
     4201 gtggcattta ggggtgactc cttcacacat actcctcctc tggatccaca ggaactggat
     4261 attctgaaaa ccgtaaagga aatcacaggg tttttgctga ttcaggcttg gcctgaaaac
     4321 aggacggacc tccatgcctt tgagaaccta gaaatcatac gcggcaggac caagcaacat
     4381 ggtcagtttt ctcttgcagt cgtcagcctg aacataacat ccttgggatt acgctccctc
     4441 aaggagataa gtgatggaga tgtgataatt tcaggaaaca aaaatttgtg ctatgcaaat
     4501 acaataaact ggaaaaaact gtttgggacc tccggtcaga aaaccaaaat tataagcaac
     4561 agaggtgaaa acagctgcaa ggccacaggc caggtctgcc atgccttgtg ctcccccgag
     4621 ggctgctggg gcccggagcc cagggactgc gtctcttgcc ggaatgtcag ccgaggcagg
     4681 gaatgcgtgg acaagtgcaa ccttctggag ggtgagccaa gggagtttgt ggagaactct
     4741 gagtgcatac agtgccaccc agagtgcctg cctcaggcca tgaacatcac ctgcacagga
     4801 cggggaccag acaactgtat ccagtgtgcc cactacattg acggccccca ctgcgtcaag
     4861 acctgcccgg caggagtcat gggagaaaac aacaccctgg tctggaagta cgcagacgcc
     4921 ggccatgtgt gccacctgtg ccatccaaac tgcacctacg gatgcactgg gccaggtctt
     4981 gaaggctgtc caacgaatgg gcctaagatc ccgtccatcg ccactgggat ggtgggggcc
     5041 ctcctcttgc tgctggtggt ggccctgggg atcggcctct tcatgcgaag gcgccacatc
     5101 gttcggaagc gcacgctgcg gaggctgctg caggagaggg agcttgtgga gcctcttaca
     5161 cccagtggag aagctcccaa ccaagctctc ttgaggatct tgaaggaaac tgaattcaaa
     5221 aagatcaaag tgctgggctc cggtgcgttc ggcacggtgt ataagggact ctggatccca
     5281 gaaggtgaga aagttaaaat tcccgtcgct atcaaggaat taagagaagc aacatctccg
     5341 aaagccaaca aggaaatcct cgatgaagcc tacgtgatgg ccagcgtgga caacccccac
     5401 gtgtgccgcc tgctgggcat ctgcctcacc tccaccgtgc agctcatcac gcagctcatg
     5461 cccttcggct gcctcctgga ctatgtccgg gaacacaaag acaatattgg ctcccagtac
     5521 ctgctcaact ggtgtgtgca gatcgcaaag ggcatgaact acttggagga ccgtcgcttg
     5581 gtgcaccgcg acctggcagc caggaacgta ctggtgaaaa caccgcagca tgtcaagatc
     5641 acagattttg ggctggccaa actgctgggt gcggaagaga aagaatacca tgcagaagga
     5701 ggcaaagtgc ctatcaagtg gatggcattg gaatcaattt tacacagaat ctatacccac
     5761 cagagtgatg tctggagcta cggggtgacc gtttgggagt tgatgacctt tggatccaag
     5821 ccatatgacg gaatccctgc cagcgagatc tcctccatcc tggagaaagg agaacgcctc
     5881 cctcagccac ccatatgtac catcgatgtc tacatgatca tggtcaagtg ctggatgata
     5941 gacgcagata gtcgcccaaa gttccgtgag ttgatcatcg aattctccaa aatggcccga
     6001 gacccccagc gctaccttgt cattcagggg gatgaaagaa tgcatttgcc aagtcctaca
     6061 gattccaact tctaccgtgc cctgatggat gaagaagaca tggacgacgt ggtggatgcc
     6121 gacgagtacc tcatcccaca gcagggcttc ttcagcagcc cctccacgtc acggactccc
     6181 ctcctgagct ctctgagtgc aaccagcaac aattccaccg tggcttgcat tgatagaaat
     6241 gggctgcaaa gctgtcccat caaggaagac agcttcttgc agcgatacag ctcagacccc
     6301 acaggcgcct tgactgagga cagcatagac gacaccttcc tcccagtgcc tgaatacata
     6361 aaccagtccg ttcccaaaag gcccgctggc tctgtgcaga atcctgtcta tcacaatcag
     6421 cctctgaacc ccgcgcccag cagagaccca cactaccagg acccccacag cactgcagtg
     6481 ggcaaccccg agtatctcaa cactgtccag cccacctgtg tcaacagcac attcgacagc
     6541 cctgcccact gggcccagaa aggcagccac caaattagcc tggacaaccc tgactaccag
     6601 caggacttct ttcccaagga agccaagcca aatggcatct ttaagggctc cacagctgaa
     6661 aatgcagaat acctaagggt cgcgccacaa agcagtgaat ttattggagc aatggtgagc
     6721 aagggcgagg cagtgatcaa ggagttcatg cggttcaagg tgcacatgga gggctccatg
     6781 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     6841 accgccaagc tgaaggtgac caagggtggc cccctgccct tctcctggga catcctgtcc
     6901 cctcagttca tgtacggctc cagggccttc accaagcacc ccgccgacat ccccgactac
     6961 tataagcagt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     7021 ggcgccgtga ccgtgaccca ggacacctcc ctggaggacg gcaccctgat ctacaaggtg
     7081 aagctccgcg gcaccaactt ccctcctgac ggccccgtaa tgcagaagaa gacaatgggc
     7141 tgggaagcgt ccaccgagcg gttgtacccc gaggacggcg tgctgaaggg cgacattaag
     7201 atggccctgc gcctgaagga cggaggccgc tacctggcgg acttcaagac cacctacaag
     7261 gccaagaagc ccgtgcagat gcccggcgcc tacaacgtcg accgcaagtt ggacatcacc
     7321 tcccacaacg aggactacac cgtggtggaa cagtacgaac gctccgaggg ccgccactcc
     7381 accggcggca tggacgagct gtacaagtaa gcggccgcgg atcctctaga gttaacatcg
     7441 agggatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     7501 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     7561 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     7621 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     7681 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     7741 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     7801 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     7861 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     7921 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     7981 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     8041 ctccccgcat cgataccgtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     8101 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     8161 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     8221 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     8281 tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac
     8341 accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     8401 tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt
     8461 gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg
     8521 cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt
     8581 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     8641 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     8701 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa
     8761 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     8821 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     8881 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     8941 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     9001 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     9061 gcggatatcc cgggacgtca ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9121 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9181 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9241 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9301 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9361 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9421 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     9481 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     9541 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     9601 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9661 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     9721 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     9781 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9841 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9901 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9961 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    10021 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    10081 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    10141 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    10201 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    10261 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10321 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10381 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    10441 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    10501 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    10561 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    10621 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10681 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    10741 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    10801 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
    10861 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
    10921 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctga
//
