LOCUS       sequence-389656-       10980 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express hCOL4A5_R1683X in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10980)
  AUTHORS   Omachi K, Kai H, Roberge M, Miner JH
  TITLE     NanoLuc reporters identify COL4A5 nonsense mutations susceptible to 
            drug-induced stop codon readthrough.
  JOURNAL   iScience. 2022 Feb 8;25(3):103891. doi: 10.1016/j.isci.2022.103891. 
            eCollection 2022 Mar 18.
  PUBMED    35243249
REFERENCE   2  (bases 1 to 10980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2022-02-8"; volume: "25(3):103891. doi"; pages: " 
            10.1016/j.isci.2022.103891. eCollection 2022 Mar 18"
COMMENT     Sequence Label: pNLF1-C_hCOL4A5-Nluc_R1683X
FEATURES             Location/Qualifiers
     source          1..10980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        135..514
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        515..718
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     669..689
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          854..986
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     969..988
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1030..1049
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1030..1048
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6152..6664
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     primer_bind     complement(6740..6758)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    6795..6916
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6806..6825)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6860..6879
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        7161..7518
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7161..7352
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7184..7204)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7369..7504
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7549..8586
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             8565..8573
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    8610..8658
                     /note="synthetic polyadenylation signal"
     promoter        8814..8918
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8919..8987,8988..9779)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9137..9156)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(9937..10525)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10017..10036)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    10641..10924
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 atattggcca ttagccatat tattcattgg ttatatagca taaatcaata ttggctattg
       61 gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat
      121 atgaccgcca tgttggcatt gattattgac tagttattaa tagtaatcaa ttacggggtc
      181 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      241 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      301 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      361 cttggcagta catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg
      421 taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca
      481 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacaccaa
      541 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
      601 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta ataaccccgc
      661 cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg
      721 tttagtgaac cgtcagatca ctagaagctt tattgcggta gtttatcaca gttaaattgc
      781 taacgcagtc agtgcttctg acacaacagt ctcgaactta agctgcagaa gttggtcgtg
      841 aggcactggg caggtaagta tcaaggttac aagacaggtt taaggagacc aatagaaact
      901 gggcttgtcg agacagagaa gactcttgcg tttctgatag gcacctattg gtcttactga
      961 catccacttt gcctttctct ccacaggtgt ccactcccag ttcaattaca gctcttaagg
     1021 ctagagtatt aatacgactc actatagggc tagcaaagcg atcgccatga aactgcgtgg
     1081 agtcagcctg gctgccggct tgttcttact ggccctgagt ctttgggggc agcctgcaga
     1141 ggctgcggct tgctatgggt gttctccagg atcaaagtgt gactgcagtg gcataaaagg
     1201 ggaaaaggga gagagagggt ttccaggttt ggaaggacac ccaggattgc ctggatttcc
     1261 aggtccagaa gggcctccgg ggcctcgggg acaaaagggt gatgatggaa ttccagggcc
     1321 accaggacca aaaggaatca gaggtcctcc tggacttcct ggatttccag ggacaccagg
     1381 tcttcctgga atgccaggcc acgatggggc cccaggacct caaggtattc ccggatgcaa
     1441 tggaaccaag ggagaacgtg gatttccagg cagtcccggt tttcctggtt tacagggtcc
     1501 tccaggaccc cctgggatcc caggtatgaa gggtgaacca ggtagtataa ttatgtcatc
     1561 actgccagga ccaaagggta atccaggata tccaggtcct cctggaatac aaggcctacc
     1621 tggtcccact ggtataccag ggccaattgg tcccccagga ccaccaggtt tgatgggccc
     1681 tcctggtcca ccaggacttc caggacctaa ggggaatatg ggcttaaatt tccagggacc
     1741 caaaggtgaa aaaggtgagc aaggtcttca gggcccacct gggccacctg ggcagatcag
     1801 tgaacagaaa agaccaattg atgtagagtt tcagaaagga gatcagggac ttcctggtga
     1861 ccgagggcct cctggacctc cagggatacg tggtcctcca ggtcccccag gtggtgagaa
     1921 aggtgagaag ggtgagcaag gagagccagg caaaagaggt aaaccaggca aagatggaga
     1981 aaatggccaa ccaggaattc ctggtttgcc tggtgatcct ggttaccctg gtgaacccgg
     2041 aagggatggt gaaaagggcc aaaaaggtga cactggccca cctggacctc ctggacttgt
     2101 aattcctaga cctgggactg gtataactat aggagaaaaa ggaaacattg ggttgcctgg
     2161 gttgcctgga gaaaaaggag agcgaggatt tcctggaata cagggtccac ctggccttcc
     2221 tggacctcca ggggctgcag ttatgggtcc tcctggccct cctggatttc ctggagaaag
     2281 gggtcagaaa ggtgatgaag gaccacctgg aatttccatt cctggacctc ctggacttga
     2341 cggacagcct ggggctcctg ggcttccagg gcctcctggc cctgctggcc ctcacattcc
     2401 tcctagtgat gagatatgtg aaccaggccc tccaggcccc ccaggatctc caggtgataa
     2461 aggactccaa ggagaacaag gagtgaaagg tgacaaaggt gacacttgct tcaactgcat
     2521 tggaactggt atttcagggc ctccaggtca acctggtttg ccaggtctcc caggtcctcc
     2581 aggatctctt ggtttccctg gacagaaagg ggaaaaagga caagctggtg caactggtcc
     2641 caaaggatta ccaggcattc caggagctcc aggtgctcca ggctttcctg gatctaaagg
     2701 tgaacctggt gatatcctca cttttccagg aatgaagggt gacaaaggag agttgggttc
     2761 ccctggagct ccagggcttc ctggtttacc tggcactcct ggacaggatg gattgccagg
     2821 gcttcctggc ccgaaaggag agcctggtgg aattactttt aagggtgaaa gaggtccccc
     2881 tgggaaccca ggtttaccag gcctcccagg gaatataggg cctatgggtc cccctggttt
     2941 cggccctcca ggcccagtag gtgaaaaagg catacaaggt gtggcaggaa atccaggcca
     3001 gccaggaata ccaggtccta aaggggatcc aggtcagact ataacccagc cggggaagcc
     3061 tggcttgcct ggtaacccag gcagagatgg tgatgtaggt cttccaggtg accctggact
     3121 tccagggcaa ccaggcttgc cagggatacc tggtagcaaa ggagaaccag gtatccctgg
     3181 aattgggctt cctggaccac ctggtcccaa aggctttcct ggaattccag gacctccagg
     3241 agcacctggg acacctggaa gaattggtct agaaggccct cctgggccac ccggctttcc
     3301 aggaccaaag ggtgaaccag gatttgcatt acctgggcca cctgggccac caggacttcc
     3361 aggtttcaaa ggagcacttg gtccaaaagg tgatcgtggt ttcccaggac ctccgggtcc
     3421 tccaggacgc actggcttag atgggctccc tggaccaaaa ggtgatgttg gaccaaatgg
     3481 acaacctgga ccaatgggac ctcctgggct gccaggaata ggtgttcagg gaccaccagg
     3541 accaccaggg attcctgggc caataggtca acctggttta catggaatac caggagagaa
     3601 gggggatcca ggacctcctg gacttgatgt tccaggaccc ccaggtgaaa gaggcagtcc
     3661 agggatcccc ggagcacctg gtcctatagg acctccagga tcaccagggc ttccaggaaa
     3721 agcaggtgcc tctggatttc caggtaccaa aggtgaaatg ggtatgatgg gacctccagg
     3781 cccaccagga cctttgggaa ttcctggcag gagtggtgta cctggtctta aaggtgatga
     3841 tggcttgcag ggtcagccag gacttcctgg ccctacagga gaaaaaggta gtaaaggaga
     3901 gcctggcctt ccaggccctc ctggaccaat ggatccaaat cttctgggct caaaaggaga
     3961 gaagggggaa cctggcttac caggtatacc tggagtttca gggccaaaag gttatcaggg
     4021 tttgcctgga gacccagggc aacctggact gagtggacaa cctggattac caggaccacc
     4081 aggtcccaaa ggtaaccctg gtctccctgg acagccaggt cttataggac ctcctggact
     4141 taaaggaacc atcggtgata tgggttttcc agggcctcag ggtgtggaag ggcctcctgg
     4201 accttctgga gttcctggac aacctggctc cccaggatta cctggacaga aaggcgacaa
     4261 aggtgatcct ggtatttcaa gcattggtct tccaggtctt cctggtccaa agggtgagcc
     4321 tggtctgcct ggatacccag ggaaccctgg tatcaaaggt tctgtgggag atcctggttt
     4381 gcccggatta ccaggaaccc ctggagcaaa aggacaacca ggccttcctg gattcccagg
     4441 aaccccaggc cctcctggac caaaaggtat tagtggccct cctgggaacc ccggccttcc
     4501 aggagaacct ggtcctgtag gtggtggagg tcatcctggg caaccagggc ctccaggcga
     4561 aaaaggcaaa cccggtcaag atggtattcc tggaccagct ggacagaagg gtgaaccagg
     4621 tcaaccaggc tttggaaacc caggaccgcc tggacttcca ggactttctg gccaaaaggg
     4681 tgatggagga ttacctggga ttccaggaaa tcctggcctt ccaggtccaa agggcgaacc
     4741 aggctttcac ggtttccctg gtgtgcaggg tcccccaggc cctcctggtt ctccgggtcc
     4801 agctctggaa ggacctaaag gcaaccctgg gccccaaggt cctcctggga gaccaggtcc
     4861 tacaggtttt caaggtctac caggtccaga aggtcctcca ggtctccctg gaaatggagg
     4921 tattaaagga gagaagggaa atccaggcca acctgggcta cctggcttgc ctggtttgaa
     4981 aggagatcaa ggaccaccag gactccaggg taatcctggc cggccgggtc tcaatggaat
     5041 gaaaggagat cctggtctcc ctggtgttcc aggattccca ggcatgaaag gacccagtgg
     5101 agtacctgga tcagctggcc ctgaggggga accgggactt attggtcctc caggtcctcc
     5161 tggattacct ggtccttcag gacagagtat cataattaaa ggagatgctg gtcctccagg
     5221 aatccctggc cagcctgggc taaagggtct accaggaccc caaggacctc aaggcttacc
     5281 aggtccaact ggccctccag gagatcctgg acgcaatgga ctccctggct ttgatggtgc
     5341 aggagggcgc aaaggagacc caggtctgcc aggacagcca ggtacccgtg gtttggatgg
     5401 tccccctggt ccagatggat tgcaaggtcc cccaggtccc cctggaacct cctctgttgc
     5461 acatggattt cttattacac gccacagcca gacaacggat gcaccacaat gcccacaggg
     5521 aacacttcag gtctatgaag gcttttctct cctgtatgta caaggaaata aaagagccca
     5581 cggtcaagac ttggggacgg ctggcagctg ccttcgtcgc tttagtacca tgcctttcat
     5641 gttctgcaac atcaataatg tttgcaactt tgcttcaaga aatgactatt cttactggct
     5701 ctctacccca gagcccatgc caatgagcat gcaaccccta aagggccaga gcatccagcc
     5761 attcattagt cgatgtgcag tatgtgaagc tccagctgtg gtgatcgcag ttcacagtca
     5821 gacgatccag attccccatt gtcctcaggg atgggattct ctgtggattg gttattcctt
     5881 catgatgcat acaagtgcag gggcagaagg ctcaggtcaa gccctagcct cccctggttc
     5941 ctgcttggaa gagtttcgtt cagctccctt catcgaatgt catgggaggg gtacctgtaa
     6001 ctactatgcc aactcctaca gcttttggct ggcaactgta gatgtgtcag acatgttcag
     6061 taaacctcag tcagaaacgc tgaaagcagg agacttgagg acacgaatta gctgatgtca
     6121 agtgtgcatg aagaggacat tctcgagcgg cgtcttcaca ctcgaagatt tcgttgggga
     6181 ctggcgacag acagccggct acaacctgga ccaagtcctt gaacagggag gtgtgtccag
     6241 tttgtttcag aatctcgggg tgtccgtaac tccgatccaa aggattgtcc tgagcggtga
     6301 aaatgggctg aagatcgaca tccatgtcat catcccgtat gaaggtctga gcggcgacca
     6361 aatgggccag atcgaaaaaa tttttaaggt ggtgtaccct gtggatgatc atcactttaa
     6421 ggtgatcctg cactatggca cactggtaat cgacggggtt acgccgaaca tgatcgacta
     6481 tttcggacgg ccgtatgaag gcatcgccgt gttcgacggc aaaaagatca ctgtaacagg
     6541 gaccctgtgg aacggcaaca aaattatcga cgagcgcctg atcaaccccg acggctccct
     6601 gctgttccga gtaaccatca acggagtgac cggctggcgg ctgtgcgaac gcattctggc
     6661 gtaaggccgc gactctaggg catgcaagct gatccggctg ctaacaaagc ccgaaaggaa
     6721 gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcggccgct
     6781 tcgagcagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     6841 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     6901 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     6961 gatgtgggag gtttttttaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgaatttta
     7021 acaaaatatt aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg
     7081 tatttcacac cgcatacgcg gatctgcgca gcaccatggc ctgaaataac ctctgaaaga
     7141 ggaacttggt taggtacctt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
     7201 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     7261 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     7321 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     7381 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     7441 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     7501 ggcctaggct tttgcaaaaa gctcgattct tctgacacta gcgccaccat gaagaagccc
     7561 gaactcaccg ctaccagcgt tgaaaaattt ctcatcgaga agttcgacag tgtgagcgac
     7621 ctgatgcagt tgtcggaggg cgaagagagc cgagccttca gcttcgatgt cggcggacgc
     7681 ggctatgtac tgcgggtgaa tagctgcgct gatggcttct acaaagaccg ctacgtgtac
     7741 cgccacttcg ccagcgctgc actacccatc cccgaagtgt tggacatcgg cgagttcagc
     7801 gagagcctga catactgcat cagtagacgc gcccaaggcg ttactctcca agacctcccc
     7861 gaaacagagc tgcctgctgt gttacagcct gtcgccgaag ctatggatgc tattgccgcc
     7921 gccgacctca gtcaaaccag cggcttcggc ccattcgggc cccaaggcat cggccagtac
     7981 acaacctggc gggatttcat ttgcgccatt gctgatcccc atgtctacca ctggcagacc
     8041 gtgatggacg acaccgtgtc cgccagcgta gctcaagccc tggacgaact gatgctgtgg
     8101 gccgaagact gtcccgaggt gcgccacctc gtccatgccg acttcggcag caacaacgtc
     8161 ctgaccgaca acggccgcat caccgccgta atcgactggt ccgaagctat gttcggggac
     8221 agtcagtacg aggtggccaa catcttcttc tggcggccct ggctggcttg catggagcag
     8281 cagactcgct acttcgagcg ccggcatccc gagctggccg gcagccctcg tctgcgagcc
     8341 tacatgctgc gcatcggcct ggatcagctc taccagagcc tcgtggacgg caacttcgac
     8401 gatgctgcct gggctcaagg ccgctgcgat gccatcgtcc gcagcggggc cggcaccgtc
     8461 ggtcgcacac aaatcgctcg ccggagcgca gccgtatgga ccgacggctg cgtcgaggtg
     8521 ctggccgaca gcggcaaccg ccggcccagt acacgaccgc gcgctaagga ggtaggtcga
     8581 gtttagactc tagccatcac gatggccgca ataaaatatc tttattttca ttacatctgt
     8641 gtgttggttt tttgtgtgaa tcgatagcga taaggatcct ctttgcgctt gcgttttccc
     8701 ttgtccagat agcccagtag ctgacattca tccggggtca gcaccgtttc tgcggactgg
     8761 ctttctacgt aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     8821 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     8881 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     8941 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     9001 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     9061 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     9121 gcactttcaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     9181 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     9241 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     9301 gtgataacac tgcggccaac ttacttctga caactatcgg aggaccgaag gagctaaccg
     9361 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     9421 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     9481 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     9541 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     9601 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     9661 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     9721 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaat
     9781 tcgaaatgac cgaccaagcg acgcccaacc ggtatcagct cactcaaagg cggtaatacg
     9841 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     9901 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     9961 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    10021 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    10081 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    10141 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    10201 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    10261 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    10321 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
    10381 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    10441 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    10501 tacgcgcaga aaaaaaggat ttcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    10561 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    10621 cacctagatc cttttatagt ccggaaatac aggaacgcac gctggatggc ccttcgctgg
    10681 gatggtgaaa ccatgaaaaa tggcagcttc agtggattaa gtgggggtaa tgtggcctgt
    10741 accctctggt tgcataggta ttcatacggt taaaatttat caggcgcgat tgcggcagtt
    10801 tttcgggtgg tttgttgcca tttttacctg tctgctgccg tgatcgcgct gaacgcgttt
    10861 tagcggtgcg tacaattaag ggattatggt aaatccactt actgtctgcc ctcgtagcca
    10921 tcgagataaa ccgcagtact ccggccacga tgcgtccggc gtagaggatc gagatcttca
//
