LOCUS       sequence-390145-       13466 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For ubiquitous expression of split-intein Gal4-C-int component under
            control of the tubulin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13466)
  AUTHORS   Ewen-Campen B, Luan H, Xu J, Singh R, Joshi N, Thakkar T, Berger B, 
            White BH, Perrimon N
  TITLE     split-intein Gal4 provides intersectional genetic labeling that is 
            repressible by Gal80.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Jun 13;120(24):e2304730120. doi: 
            10.1073/pnas.2304730120. Epub 2023 Jun 5.
  PUBMED    37276389
REFERENCE   2  (bases 1 to 13466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-06-13"; volume: "120(24):e2304730120. 
            doi"; pages: " 10.1073/pnas.2304730120. Epub 2023 Jun 5"
COMMENT     Sequence Label: pTub-split-intein-Gal4[21-881]-C-int
FEATURES             Location/Qualifiers
     source          1..13466
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(675..693)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1145..1216,1618..1891,1966..2620,2682..2997,3201..3332,
                     3403..4017)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4792..4825
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             9897..10241
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     intron          10340..10405
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10535..10555
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10827..10961
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10864..10883)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10918..10937
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    10990..11059
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(11278..11296)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11317..11333)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11317..11333)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11330..11352)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11341..11357
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11365..11371,11372..11389,11390..11395))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11410..11431
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11548..11565)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11719..12307)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11799..11818)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12478..13269,13270..13338))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13101..13120
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13339..13443)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccaa gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggctctaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcctt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agtattacat aacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgccattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatctggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagata gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaagat aaataaattt catttaactc gcgaatatta atgagatgcg
     3181 agtaacattt taatttgcag atggttgcca tcttgattgg cctcatcttt ttgggccaac
     3241 aactcacgca agtgggtgtg atgaatatca acggagccat cttcctcttc ctgaccaaca
     3301 tgacctttca aaacgtcttt gccacgataa atgtaagtca tgtttagaat acatttgcat
     3361 ttcaataatt tactaacttt ctaatgaatc gattcgattt aggtgttcac ctcagagctg
     3421 ccagttttta tgagggaggc ccgaagtcga ctttatcgct gtgacacata ctttctgggc
     3481 aaaacgattg ccgaattgcc gctttttctc acagtgccac tggtcttcac ggcgattgcc
     3541 tatccgatga tcggactgcg ggccggagtg ctgcacttct tcaactgcct ggcgctggtc
     3601 actctggtgg ccaatgtgtc aacgtccttc ggatatctaa tatcctgcgc cagctcctcg
     3661 acctcgatgg cgctgtctgt gggtccgccg gttatcatac cattcctgct ctttggcggc
     3721 ttcttcttga actcgggctc ggtgccagta tacctcaaat ggttgtcgta cctctcatgg
     3781 ttccgttacg ccaacgaggg tctgctgatt aaccaatggg cggacgtgga gccgggcgaa
     3841 attagctgca catcgtcgaa caccacgtgc cccagttcgg gcaaggtcat cctggagacg
     3901 cttaacttct ccgccgccga tctgccgctg gactacgtgg gtctggccat tctcatcgtg
     3961 agcttccggg tgctcgcata tctggctcta agacttcggg cccgacgcaa ggagtagccg
     4021 acatatatcc gaaataactg cttgtttttt ttttttacca ttattaccat cgtgtttact
     4081 gtttattgcc ccctcaaaaa gctaatgtaa ttatatttgt gccaataaaa acaagatatg
     4141 acctatagaa tacaagtatt tccccttcga acatccccac aagtagactt tggatttgtc
     4201 ttctaaccaa aagacttaca cacctgcata ccttacatca aaaactcgtt tatcgctaca
     4261 taaaacaccg ggatatattt tttatataca tacttttcaa atcgcgcgcc ctcttcataa
     4321 ttcacctcca ccacaccacg tttcgtagtt gctctttcgc tgtctcccac ccgctctccg
     4381 caacacattc accttttgtt cgacgacctt ggagcgactg tcgttagttc cgcgcgattc
     4441 ggttcgctca aatggttccg agtggttcat ttcgtctcaa tagaaattag taataaatat
     4501 ttgtatgtac aatttatttg ctccaatata tttgtatata tttccctcac agctatattt
     4561 attctaattt aatattatga ctttttaagg taattttttg tgacctgttc ggagtgatta
     4621 gcgttacaat ttgaactgaa agtgacatcc agtgtttgtt ccttgtgtag atgcatctca
     4681 aaaaaatggt gggcataata gtgttgttta tatatatcaa aaataacaac tataataata
     4741 agaatacatt taatttagaa aatgcttgga tttcactgga actaggctag cataacttcg
     4801 tataatgtat gctatacgaa gttatgctag cggatccaag cttgcggccg caattctata
     4861 ttctaaaaac acaaatgata cttctaaaaa aaaatcatga atggcatcaa ctctaagctt
     4921 gcacaggtcc tgttcgataa cgtcgtactc gggaatcacc tttaacttgt ccgctattgg
     4981 gtatttgtcc ttaaacacat tcgtaatctc tcccacaatc attgccctgg cctctgcaat
     5041 gtaaaaagta tttataaaat atcgctggct ctaaaggaaa catattaatc aaagtattga
     5101 ggctttatca atctaacatt tacaagtagt cagaccacaa tttttagact gcacttctgc
     5161 gtaagtaaat gaaatgaaat ggtgacctac tcttgggtag ccgtactgtt atatgctgac
     5221 gagccttcac ctgggcctgc tttttctcct gtttcgtctg atgcgtcgga tgcggctgga
     5281 ctggctgatt gttgggattg ggttttggat gctgctccga aaggtgggcc acagcttttg
     5341 cgatggtggt gcccactatg cggttgctgc aggaactaca gctgaatgtt cgctttattg
     5401 tctgcagctc ctggggctgg acgaaggcca cgccctcacc cggtgcggcc atctcctgcg
     5461 gcaaaacgta tgtgggattc ctgtccaagt agctctcgat gcccaccata atgcgctcca
     5521 gcaggttggc caggtgcggg tctagctgcg gcggattcgt cgtctggttc actgagttta
     5581 gggtctgcag aaagaagtgt gcctccatgg taagcggatt catctgcacg atcagcgggc
     5641 tttagtctaa aaattcgaat gatacaaact cgtactcacg taaagcagtt tgtttggcat
     5701 catctcggcc atcgtcttat tcttcttcgt ggcctttgtt accggagcgg ctatgttggg
     5761 cgcggtggag cccggaagct gaaaggttaa tcgaatgcgt taacttttct gcaactcgaa
     5821 agtttgccgc ctttgttcga ctgccaataa ctgttgattc gaaaattcga atcgaagcgg
     5881 ttgaatttcg tagggtggcc aactacacca aagttcgccg gcggtgtatg catgtgagtg
     5941 tgagtgtgac agtgggtgta cgtgtgcttt aagcgacaca atcgcaccgc gatgtacata
     6001 tttttgtagt aatgtgtgtg catttttcgt ccggatcacg gctccataaa ataaatcata
     6061 tcacagggct cagggatttg tgagtagaag ttcccgccac ttaaacgtca cttgtgaaat
     6121 cgccatcgac ggtcaacttt tactcacact ggtctttcgc ggacggaccg tctcaaagta
     6181 ctgcctttct gcgttggaaa acatcgcctt tttcgtccaa aaggagtccc caggttcgat
     6241 ccgcatggcg ttgtgcgtgc gtgcctttct tttcaaatga ttacggctat taacttgggg
     6301 gcgttaagtt ggaaacacgt aaattgcaga ctgcgattag agtgaccatg agtaggagtt
     6361 caaaatctcc tgacatcatt ttcttaaaac ctgctttgtt ttttacattt ctatttaata
     6421 taactcctat ttgaataaaa aaacaaaaca agtttagatg ttaagatatt aactacatcc
     6481 tttgctccaa agggagaggg gaagttatgg agttaattaa tttgctgttg gaaatcaata
     6541 tggagtcaga aatataatga tttactaaac cttattgaat cggtaacgat gcgaatttat
     6601 attaaaatag cttttatgaa acattcaaca aaaatattat taatgttggc ccactttagc
     6661 aaccggttag gtctaccggt tgggcaagca aagattcacg ccctggttcg agtcccaact
     6721 agtcctgcaa aataccgcag caagttttag agagaccaag tgccattacc tctcccactt
     6781 cagttatcgg ttatgcggcg tttaagtcga cagcttgccg tctctagctc cggtgcctat
     6841 ataaagcagc ccgctttcca catttcatat tcgttttacg tttgtcaagc ctcatagccg
     6901 gcagttcgaa cgtatacgct ctctgagtca gacctcgaaa tcgtagctct acacaattct
     6961 gtgaattttc cttgtcgcgt gtgaaacact tccaataaaa actcctgcag gtgagtactt
     7021 taaaaaaaaa tctagtgaaa taatgctgaa aagaaatttg tgtgggcaaa attcaatggg
     7081 caaaaacgcg atgcggcttt ttctcaaaat ggcggccggc ctgcgttttt tcctcaaaag
     7141 tgatgacgtc atgcctgttt tttttttttt gttcgcaatg aggaatggct cttaaaatct
     7201 actagataaa aaaaatattc attatttcta tgctgctgga acgcttcatt aatcttaaaa
     7261 attctaaatt cggttaccat gatacttcga cgcataactg tagattttgg atagaattaa
     7321 agagaaaatg gcgagagagt aaaattccgg cgtcggcaaa gtagagcaaa aaaatcagta
     7381 taccatttag ctacctctct cactcgcacg cagtgccggc tcaagttggg cgcggctctg
     7441 caattatcga ttttcttggg gtgtgtaact aatcatccgt tttcccttcc tcctcatcca
     7501 cagcgtgaag gtacccgccc ggggatcaga tccgcggccg caacatgatg ctgaagaaga
     7561 tcctgaagat cgaggagctg gatgagcgcg agctgatcga tatcgaggtg agcggcaacc
     7621 acctgttcta cgccaacgat atcctgaccc acaactgctc caaagaaaaa ccgaagtgcg
     7681 ccaagtgtct gaagaacaac tgggagtgtc gctactctcc caaaaccaaa aggtctccgc
     7741 tgactagggc acatctgaca gaagtggaat caaggctaga aagactggaa cagctatttc
     7801 tactgatttt tcctcgagaa gaccttgaca tgattttgaa aatggattct ttacaggata
     7861 taaaagcatt gttaacagga ttatttgtac aagataatgt gaataaagat gccgtcacag
     7921 atagattggc ttcagtggag actgatatgc ctctaacatt gagacagcat agaataagtg
     7981 cgacatcatc atcggaagag agtagtaaca aaggtcaaag acagttgact gtatcgattg
     8041 actcggcagc tcatcatgat aactccacaa ttccgttgga ttttatgccc agggatgctc
     8101 ttcatggatt tgattggtct gaagaggatg acatgtcgga tggcttgccc ttcctgaaaa
     8161 cggaccccaa caataatggg ttctttggcg acggttctct cttatgtatt cttcgatcta
     8221 ttggctttaa accggaaaat tacacgaact ctaacgttaa caggctcccg accatgatta
     8281 cggatagata cacgttggct tctagatcca caacatcccg tttacttcaa agttatctca
     8341 ataattttca cccctactgc cctatcgtgc actcaccgac gctaatgatg ttgtataata
     8401 accagattga aatcgcgtcg aaggatcaat ggcaaatcct ttttaactgc atattagcca
     8461 ttggagcctg gtgtatagag ggggaatcta ctgatataga tgttttttac tatcaaaatg
     8521 ctaaatctca tttgacgagc aaggtcttcg agtcaggttc cataattttg gtgacagccc
     8581 tacatcttct gtcgcgatat acacagtgga ggcagaaaac aaatactagc tataattttc
     8641 acagcttttc cataagaatg gccatatcat tgggcttgaa tagggacctc ccctcgtcct
     8701 tcagtgatag cagcattctg gaacaaagac gccgaatttg gtggtctgtc tactcttggg
     8761 agatccaatt gtccctgctt tatggtcgat ccatccagct ttctcagaat acaatctcct
     8821 tcccttcttc tgtcgacgat gtgcagcgta ccacaacagg tcccaccata tatcatggca
     8881 tcattgaaac agcaaggctc ttacaagttt tcacaaaaat ctatgaacta gacaaaacag
     8941 taactgcaga aaaaagtcct atatgtgcaa aaaaatgctt gatgatttgt aatgagattg
     9001 aggaggtttc gagacaggca ccaaagtttt tacaaatgga tatttccacc accgctctaa
     9061 ccaatttgtt gaaggaacac ccttggctat cctttacaag attcgaactg aagtggaaac
     9121 agttgtctct tatcatttat gtattaagag attttttcac taattttacc cagaaaaagt
     9181 cacaactaga acaggatcaa aatgatcatc aaagttatga agttaaacga tgctccatca
     9241 tgttaagcga tgcagcacaa agaactgtta tgtctgtaag tagctatatg gacaatcata
     9301 atgtcacccc atattttgcc tggaattgtt cttattactt gttcaatgca gtcctagtac
     9361 ccataaagac tctactctca aactcaaaat cgaatgctga gaataacgag accgcacaat
     9421 tattacaaca aattaacact gttctgatgc tattaaaaaa actggccact tttaaaatcc
     9481 agacttgtga aaaatacatt caagtactgg aagaggtatg tgcgccgttt ctgttatcac
     9541 agtgtgcaat cccattaccg catatcagtt ataacaatag taatggtagc gccattaaaa
     9601 atattgtcgg ttctgcaact atcgcccaat accctactct tccggaggaa aatgtcaaca
     9661 atatcagtgt taaatatgtt tctcctggct cagtagggcc ttcacctgtg ccattgaaat
     9721 caggagcaag tttcagtgat ctagtcaagc tgttatctaa ccgtccaccc tctcgtaact
     9781 ctccagtgac aataccaaga agcacacctt cgcatcgctc agtcacgcct tttctagggc
     9841 aacagcaaca gctgcaatca ttagtgccac tgaccccgtc tgctttgttt ggtggcgcca
     9901 attttaatca aagtgggaat attgctgata gctcattgtc cttcactttc actaacagta
     9961 gcaacggtcc gaacctcata acaactcaaa caaattctca agcgctttca caaccaattg
    10021 cctcctctaa cgttcatgat aacttcatga ataatgaaat cacggctagt aaaattgatg
    10081 atggtaataa ttcaaaacca ctgtcacctg gttggacgga ccaaactgcg tataacgcgt
    10141 ttggaatcac tacagggatg tttaatacca ctacaatgga tgatgtatat aactatctat
    10201 tcgatgatga agatacccca ccaaacccaa aaaaagagta ataactcgag ggtacctcta
    10261 gaggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac
    10321 agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact
    10381 gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg
    10441 gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga
    10501 tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga
    10561 ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag
    10621 aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa
    10681 aattatggaa aaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca
    10741 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
    10801 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
    10861 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    10921 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccactagtgt cgacgatgta
    10981 ggtcacggtc tcgaagccgc ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc
    11041 gtactccacc tcacccatct ggtccatcat gatgaacggg tcgaggtggc ggtagttgat
    11101 cccggcgaac gcgcggcgca ccgggaagcc ctcgccctcg aaaccgctgg gcgcggtggt
    11161 cacggtgagc acgggacgtg cgacggcgtc ggcgggtgcg gatacgcggg gcagcgtcag
    11221 cgggttctcg acggtcacgg cgggcatgtc gacactagtt ctagccagct tttgttccct
    11281 ttagtgaggg ttaatttcga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
    11341 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
    11401 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
    11461 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
    11521 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
    11581 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
    11641 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
    11701 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    11761 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    11821 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    11881 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    11941 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    12001 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    12061 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    12121 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
    12181 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    12241 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    12301 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    12361 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    12421 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    12481 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    12541 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    12601 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    12661 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    12721 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    12781 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    12841 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    12901 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    12961 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    13021 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    13081 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    13141 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    13201 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    13261 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    13321 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    13381 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    13441 gcgcacattt ccccgaaaag tgccac
//
