LOCUS       sequence-390244-        5611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drives specific expression in the AWC (OFF) neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 5611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pMZ10
FEATURES             Location/Qualifiers
     source          1..5611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2669..2818
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        3356..3460
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3461..3529,3530..4321)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3679..3698)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4492..5080
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4981..5000
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5234..5251
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5368..5389
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5404..5409,5410..5427,5428..5434)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5442..5458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5447..5469
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5466..5482
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5466..5482
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggccgcgt aaaacacagc agaaaaatat
       61 tatcttaaat agttatcgta cctctatctt taaaggtgca ttttatttgg agcattttct
      121 gatttatttt cgtcataact tagcttcgtc ataaaaataa gcttactcat cccccattct
      181 atacaaactt attttcaatt gtttagctct tattcaactt aatcctatac accctattct
      241 tcttaacatt ccctaaatgt caaactctaa aacatatgag ctattaagcg ataattagag
      301 ggtatacctg catttgtaaa tttaactgaa atttgatccc atgaagtgtc ggctgcagca
      361 aaaattttct gtgaaacaca tgtattctga aaccagaaag gctttaatca ctctataatg
      421 aaaaaaagtg atccgagtat caaaagccgc cgagctcacg gtggacataa gtagggtaac
      481 gcttttttga aacattactt tttacatttt ttcacaaaaa tgtttcctac tgtttttacg
      541 ccactataaa taaaatgcgt ttcccaaaat atttagttgt gaaatgctaa gaattcacaa
      601 aaaaaatcca aaaaattgct gtttcgcgat tatttcaata caactgcaca aaatggaaat
      661 tttaatacta gcgatacttt ttgcagtggt cttgaaataa tagcgaaaca gcaatttttt
      721 ggttgagatt tttttattct tttcattgaa atcttttgaa aaaactatat ttattataat
      781 ggcgtaaaaa cagcaaaaaa atatttgttc aaataaatac cgtacctata tttaaaggcg
      841 caatttccga tttattttcg tcttaacttt gtcataaatg ccttcgtctt tgtcagatta
      901 tcgtcataaa aataagctta ctcaaaatat ttagttgact tatgctaaaa atgcaaaaat
      961 gcaaaaaaaa acacaaaaaa ttgctgtttc cgatgaaaac aaaaatgctg ttttacgatg
     1021 aaaaccaaaa acagtaagtt ttgcagtggt cttgaaataa tagcgaaaca gcaaattttt
     1081 cgggttgtgg tttttcgatt tggtgcttga atttcacttc gttttaatca tattttttga
     1141 agaaactata tttatttata gtggtgtaaa aatagcagaa aaattattgt tcaaataaat
     1201 atcgtacctc tatcttcaaa ggcacatgtc atttggagcg aatttcagat ttattgtcgt
     1261 cataactttg cttcgtcata aaaataagct ttctcatccc ccattcttta caaatatatt
     1321 ttcaattgtt tagctcttat tcaacttaat cctatacccc ctattctcct tagcattcct
     1381 taaatatcaa actctaaaac aattgagcta ttgagcgata attagaggga ctactgacgc
     1441 ttgtaaattt aactaaaatt tgatcccatg aagtgtcggc tgctgcagca tgtttttgtg
     1501 aaacacatgt atttcgaaac cagaaaggct ttaatcactc tataatgaaa aaaccatttg
     1561 agccgagtat caaaagccgc cgagctcatg gtggacgaaa gtagggtaac gctttttttt
     1621 ttgaaaaatt accttttacg gtttttcacg aaaatgtttc ctactgtttt tacgccactt
     1681 taaatcaaat gcgtttctaa aaatatttag ttgacttgct aaaaatggaa aaaaaaactc
     1741 caaaaaattg atgtttcgcg attatttcaa ggcaactata caaaatggta attttaatac
     1801 ctttctcgat ttaatttcgt cataactttg cttcgtcata aaaataagct tactcatccc
     1861 ccattctata caaacttatt ttcaattgtt tagctcttat tcaactaaat cctattcccc
     1921 ctattctcct taacattcct taaatatcaa actctaaaac aaatgatcta ttgacgtctg
     1981 atctactggg tataaaaacc ccttaattcc tacctataac ctctctttat caccaaggcg
     2041 cgcctctaga ggatccccgg gattggccaa aggacccaaa ggtatgtttc gaatgatact
     2101 aacataacat agaacatttt caggaggacc cttggctagg atgggcccta aaaagaagcg
     2161 taaagctagc atgaagctgt tgtcttcaat ggaacaagct tgcgacatat gccggttaaa
     2221 aaaactcagg tgctcgaagg aaaagcccaa gtgtgccaag tgtctgaaga acaactcgga
     2281 gtgccgttat tctcccaagg ctatgaggtc cccactgacc agggcgcatc tgacagaggt
     2341 agaatcaagg ttggaaaaat tggaagattt gtttctgctc atgtttcctc gggaaaacct
     2401 ggacagcatt ttgaacatgg actctttgga cgaagtgaaa gtaatgttga aacaattata
     2461 tatgcaggac tatggtgaca cggatgctgc tatggatagc ttgaccccta tggaaaatgg
     2521 catgtctgaa gggccgagaa aacacatgaa gactgcgaca tcatcgccaa aagtggatga
     2581 agatggaagc caaagtcagt tgagtgtatc gccggaattc ccgctgcagt ctggtggcgg
     2641 aggggctcct aggggttccg gacgggctga cgcattggac gattttgatc tggatatgct
     2701 gggaagtgac gccctcgatg attttgacct tgacatgctt ggttcggatg cccttgatga
     2761 ctttgacctc gacatgctcg gcagtgacgc ccttgatgat ttcgacctgg acatgctgat
     2821 taactaaggt accgaattcg ctagccggcc atacaagtaa tccggatgat cgacgccaac
     2881 gtcgttgaat tttcaaattt taaatactga atatttgttt tttttcctat tatttattta
     2941 ttctctttgt gttttttttc ttgctttcta aaaaattaat tcaatccaaa tctaaacatt
     3001 tttttttctc tttccgtctc ccaattcgta ttccgctcct ctcatctgaa cacaatgtgc
     3061 aagtttattt atcttctcgc tttcatttca ttaggacgtg gggggaattg gtggaagggg
     3121 gaaacacaca aaaggatgat ggaaatgaaa taaggacaca caatatgcaa caacattcaa
     3181 ttcagaaata tggaggaagg tttaaaagaa aacataaaaa tatatagagg aggaaggaaa
     3241 actagtaaaa aataagcaaa gaaattaggc gaacgatgag aattgtcctc gcttgggccc
     3301 aaaaggcctt ttttttttgg cgccgacgtc aggtggcact tttcggggaa atgtgcgcgg
     3361 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3421 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     3481 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     3541 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     3601 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     3661 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     3721 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     3781 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     3841 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     3901 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     3961 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     4021 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     4081 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     4141 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     4201 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     4261 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     4321 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     4381 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     4441 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4501 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4561 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4621 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4681 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4741 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4801 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4861 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4921 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4981 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     5041 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     5101 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     5161 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     5221 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     5281 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     5341 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     5401 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     5461 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
     5521 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
     5581 gatggatacg ctaacaactt ggaaatgaaa t
//
