LOCUS       sequence-390247-        5643 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Voltage-sensitive fluorescent protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 5643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pHW450
FEATURES             Location/Qualifiers
     source          1..5643
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    36..130
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    169..263
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    302..396
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3388..3492
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3493..3561,3562..4353)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3711..3730)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4524..5112
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5013..5032
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5266..5283
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5400..5421
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5436..5441,5442..5459,5460..5466)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5474..5490
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5479..5501
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5498..5514
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5498..5514
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcta gcccatgatt acgccaagct acgggcggag tactgtcctc cgagcggagt
       61 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
      121 gtcctccgag cggagactcc ctagcccatg attacgccaa gctacgggcg gagtactgtc
      181 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
      241 cgagcggagt actgtcctcc gagcggagac tccctagccc atgattacgc caagctacgg
      301 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
      361 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gactccctag gggcggccgc
      421 actgactggg ccggccaaaa atgcataagg ttttgctggc actgttcttt atctttctgg
      481 caccagcatc cgcactggca gtctccgaac cggcctgcag gtcgattttt tgcaaattac
      541 gagcgttgta gggggcggag cgataggtcc tataggtttt ggtatatcat cattcattca
      601 ttcattggta cattcattta cccaccttcc tctttctgag cttctctgga gttctgtgct
      661 tcctttttcc cttatcttta tactgtaatt tttaactttc aggcattgat tggcgcgcct
      721 ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg atactaacat
      781 aacatagaac attttcagga ggacccttgg ctagcgtcga cggtaccggt aaaaatgagg
      841 agccgctttg tgaagaaaga tggtcattgc aatgttcagt ttatcaacgt ggtctccaag
      901 ggagaggagc tcatcaagga gaacatgcac atgaagctct acatggaggg aaccgtcaac
      961 aaccaccact tcaagtgcac ctccgaggga gagggaaagc catacgaggg aacccaaacc
     1021 atgcgtatca aggccgtcga gggaggacca ctcccattcg ccttcgacat cctcgccacc
     1081 tccttcatgt acggatccaa gaccttcatc aaccacaccc aaggaatccc agactttttc
     1141 aagcaatcct tcccagaggg attcacctgg gagcgtgtca ccacctacga ggacggagga
     1201 gtcctcaccg ccacccaaga cacctccctc caagacggat gcctcatcta caacgtcaag
     1261 atccgtggag tcaacttccc atccaacgga ccagtcatgc aaaagaagac cctcggatgg
     1321 gaggcctcca ccgagaccct ctacccagcc gacggaggac tcgagggacg tgccgacatg
     1381 gccctcaagc tcgtcggagg aggacacctc atctgcaacc tcaagaccac ctaccgttcc
     1441 aagaagccag ccaagaacct caagatgcca ggagtctact acgtcgaccg tcgtctcgag
     1501 cgtatcaagg aggccgacaa ggagacctac gtcgagcaac acgaggtcgc cgtcgcccgt
     1561 tactgcgacc tcccatccaa gctcggacac cgtgaggaac gaatagatat acccgagatt
     1621 tcaggtctat ggtggggcga gaatgaacat ggagtggacg atgggagaat ggagatccca
     1681 accaccggag tcggacgtgt ccaattccgt gtccgtgccg tcatcgacca cctcggaatg
     1741 cgtgtcttcg gagtcttcct catcttcctc gacatcatcc tcatgatcat cgacctctcc
     1801 ctcccaggaa agtccgagtc ctcccaatcc ttctacgacg gaatggccct cgccctctcc
     1861 tgctacttca tgctcgacct cggactccgt atcttcgcct acggaccaaa gaacttcttc
     1921 accaacccat gggaggtcgc cgacggactc atcatcgtcg tcaccttcgt cgtcaccatc
     1981 ttctacaccg tcctcgacga gtacgtccaa gagaccggag ccgacggact cggacaactc
     2041 gtcgtcctcg cccgtctcct ccgtgtcgtc cgtctcgccc gtatcttcta ctcccaccaa
     2101 caaatgaagg cctcctcccg tcgtaccatc tcccaatgcg gacgtatggt ctccaaggga
     2161 gaggagctct tcaccggagt cgtcccaatc ctcgtcgagc tcgacggaga cgtcaacgga
     2221 cacaagttct ccgtctccgg agagggagag ggagacgcca cctacggaaa gctcaccctc
     2281 aagttcatct gcaccaccgg aaagctccca gtcccatggc caaccctcgt caccaccttc
     2341 ggatacggac tcatgtgctt cgcccgttac ccagaccaca tgaagcaaca cgacttcttc
     2401 aagtccgcca tgccagaggg atacgtccaa gagcgtacca tcttcttcaa ggacgacgga
     2461 aactacaaga cccgtgccga ggtcaagttc gagggagaca ccctcgtcaa ccgtatcgag
     2521 ctcaagggaa tcgacttcaa ggaggacgga aacatcctcg gacacaagct cgagtacaac
     2581 tacaactccc acaacgtcta catcatggcc gacaagcaaa agaacggaat caaggtcaac
     2641 ttcaagatcc gtcacaacat cgaggacgga tccgtccaac tcgccgacca ctaccaacaa
     2701 aacaccccaa tcggagacgg accagtcctc ctcccagaca accactacct ctcctaccaa
     2761 tccaagctct ccaaggaccc aaacgagaag cgtgaccaca tggtcctcct cgagttcgtc
     2821 accgccgccg gaatcaccct cggaatggac gagctctaca agtaagaatt cgctagccgg
     2881 ccatacaagt aatccggatg atcgacgcca acgtcgttga attttcaaat tttaaatact
     2941 gaatatttgt tttttttcct attatttatt tattctcttt gtgttttttt tcttgctttc
     3001 taaaaaatta attcaatcca aatctaaaca tttttttttc tctttccgtc tcccaattcg
     3061 tattccgctc ctctcatctg aacacaatgt gcaagtttat ttatcttctc gctttcattt
     3121 cattaggacg tggggggaat tggtggaagg gggaaacaca caaaaggatg atggaaatga
     3181 aataaggaca cacaatatgc aacaacattc aattcagaaa tatggaggaa ggtttaaaag
     3241 aaaacataaa aatatataga ggaggaagga aaactagtaa aaaataagca aagaaattag
     3301 gcgaacgatg agaattgtcc tcgcttgggc ccaaaaggcc tttttttttt ggcgccgacg
     3361 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     3421 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     3481 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     3541 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     3601 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     3661 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     3721 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     3781 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     3841 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     3901 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     3961 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     4021 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     4081 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     4141 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     4201 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     4261 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     4321 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     4381 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     4441 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     4501 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     4561 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     4621 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     4681 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     4741 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     4801 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     4861 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     4921 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4981 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     5041 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5101 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5161 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     5221 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     5281 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     5341 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     5401 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     5461 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     5521 tacgccaagc tgtaagttta aacatgatct tactaactaa ctattctcat ttaaattttc
     5581 agagcttaaa aatggctgaa atcactcaca acgatggata cgctaacaac ttggaaatga
     5641 aat
//
