LOCUS       sequence-390250-        5675 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drives specific expression in the URB neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 5675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pWP066
FEATURES             Location/Qualifiers
     source          1..5675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2733..2882
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        3420..3524
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3525..3593,3594..4385)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3743..3762)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4556..5144
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5045..5064
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5298..5315
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5432..5453
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5468..5473,5474..5491,5492..5498)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5506..5522
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5511..5533
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5530..5546
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5530..5546
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggccagtg agagatagtt gaacacagca
       61 tttcgacgtg ggtggatgct cctgaaactt ttaaaagata aatacttccg tttgttgact
      121 ttaaatattc agtatttgat ctcttaatgg acatgaactt atccataaga ttcgtgattt
      181 ccgcttctgg gggtgtatcc gaaaagggaa attacgtttt cctagtaaaa tgtgcaaagc
      241 ttttatattc atccttgtaa agtcgaagtc tttctaacta aaaggacgct ataataaact
      301 caaaatggac caaaaaaata tcaagacttt tccaaaacaa aaaaattaac ccacatttta
      361 ttcaatcact gtttcaattt cgcaatttca tcaacaacac acattaccat tattaggaat
      421 ttcaaatttt ttttgaaaag tcttgttaaa aacattttct tttagggtcg ttttgagtag
      481 aatacatttt ttgtacgaaa atctcaaata atcactttca ctatccagac tgacgtattt
      541 cattcacaca tccccgaaag acgagatttt gtgctttgaa cactaactcc tacacctttc
      601 ttttgcattc ttgtagcatt ctgcttccga tattcacgtc acacatgact tggttgactc
      661 acattgttcc cgagatcttg aaagtgtgaa acaagaattc atcacttttg ttttcctgat
      721 aatccaaaaa aacaagttca ttgtttttat ctagcgcttc tcaaattata attctgactg
      781 gcggtgctct tttattatga aaattttttt tgcaataagt tctttggtgc accatgttca
      841 catattctat aaaatttcaa gaattaaaaa cccagagggg gtccttaaaa tatttttagc
      901 gttttgctca aaacacttcg tttattcttg gctgtccccc tcttggtctt atgatcatcc
      961 actttttttt ttcgaaaaaa aaatacacgt catagtaagg tcttcaggag gacctcccga
     1021 tttagaccta ccactcctcc aatcgtgtga ctagattttc aatatttatt atatattcta
     1081 cggatgaata gagaattttg tgacatctgc acgggctctc agatttatat tcagttgcaa
     1141 aaagaaagtt caatacacgg ttaattgggt tttattattt cttattttca tccttgtgac
     1201 gtttggattt cgtttttttt ttcaaatatt ttttttcgat tctcggtttc tcaaaattct
     1261 gcagaacttc tcgtcttgac caaataattc tcaaaaaagc tggttctaat tatttcgtga
     1321 cgtgacttat atcgcgaaat tttcacatcg aagaagaaga aaaatatcaa aattatatta
     1381 ttcgaaaaca agtggttttg tggaattttg tgaatcatat tatgaataag ctcaaaattg
     1441 tttcagattt tggtaaattg cttaatttcc aaacaatgtg gactttcaaa aaattacgag
     1501 aattccatat tgagtgttaa tatctctaaa atgatagtac aaaaagttta aactaaattt
     1561 aaatttgaaa aaaaaatcgc cgaaaagaaa cgtttttttc agttgactca cttgagtcag
     1621 ttgagtccaa ccaattttgc tacaatttta cgcgaatttt tagttttcag aaactttcgt
     1681 ataactatgc aggtatatta ccagttaatt agactaattt gtgtggtttt ttttcaattc
     1741 caaaaatgtg gacgatactt taatattaaa atccacgagt ctgcttttta gtcattttac
     1801 aatttcgaaa aaaaaaatcg gcgccattgt tattgaagga agcattttgc atttccggct
     1861 caattctcaa gtcacaggga cccactcatt ttgtagtcta cggcgaaaaa aggaatttct
     1921 atttggtatt ggtgtatagt attcgtcttc ttcttcaatt cgcatccatc cgtgaacacc
     1981 ctcctcccga aaaatcccgc ccctattatt tcatgatttc tactagtttt ctcttgttgt
     2041 gtcaattcga gaatatttta ttattttaat ttattttctg gtttccttca gaaaatgatg
     2101 ggcgcgcctc tagaggatcc ccgggattgg ccaaaggacc caaaggtatg tttcgaatga
     2161 tactaacata acatagaaca ttttcaggag gacccttggc taggatgggc cctaaaaaga
     2221 agcgtaaagc tagcatgaag ctgttgtctt caatggaaca agcttgcgac atatgccggt
     2281 taaaaaaact caggtgctcg aaggaaaagc ccaagtgtgc caagtgtctg aagaacaact
     2341 cggagtgccg ttattctccc aaggctatga ggtccccact gaccagggcg catctgacag
     2401 aggtagaatc aaggttggaa aaattggaag atttgtttct gctcatgttt cctcgggaaa
     2461 acctggacag cattttgaac atggactctt tggacgaagt gaaagtaatg ttgaaacaat
     2521 tatatatgca ggactatggt gacacggatg ctgctatgga tagcttgacc cctatggaaa
     2581 atggcatgtc tgaagggccg agaaaacaca tgaagactgc gacatcatcg ccaaaagtgg
     2641 atgaagatgg aagccaaagt cagttgagtg tatcgccgga attcccgctg cagtctggtg
     2701 gcggaggggc tcctaggggt tccggacggg ctgacgcatt ggacgatttt gatctggata
     2761 tgctgggaag tgacgccctc gatgattttg accttgacat gcttggttcg gatgcccttg
     2821 atgactttga cctcgacatg ctcggcagtg acgcccttga tgatttcgac ctggacatgc
     2881 tgattaacta aggtaccgaa ttcgctagcc ggccatacaa gtaatccgga tgatcgacgc
     2941 caacgtcgtt gaattttcaa attttaaata ctgaatattt gttttttttc ctattattta
     3001 tttattctct ttgtgttttt tttcttgctt tctaaaaaat taattcaatc caaatctaaa
     3061 catttttttt tctctttccg tctcccaatt cgtattccgc tcctctcatc tgaacacaat
     3121 gtgcaagttt atttatcttc tcgctttcat ttcattagga cgtgggggga attggtggaa
     3181 gggggaaaca cacaaaagga tgatggaaat gaaataagga cacacaatat gcaacaacat
     3241 tcaattcaga aatatggagg aaggtttaaa agaaaacata aaaatatata gaggaggaag
     3301 gaaaactagt aaaaaataag caaagaaatt aggcgaacga tgagaattgt cctcgcttgg
     3361 gcccaaaagg cctttttttt ttggcgccga cgtcaggtgg cacttttcgg ggaaatgtgc
     3421 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     3481 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     3541 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     3601 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     3661 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     3721 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     3781 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     3841 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     3901 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     3961 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     4021 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     4081 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     4141 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     4201 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     4261 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     4321 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     4381 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     4441 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     4501 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     4561 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     4621 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     4681 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     4741 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     4801 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     4861 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     4921 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     4981 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     5041 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     5101 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     5161 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     5221 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     5281 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     5341 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     5401 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     5461 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     5521 aatttcacac aggaaacagc tatgaccatg attacgccaa gctgtaagtt taaacatgat
     5581 cttactaact aactattctc atttaaattt tcagagctta aaaatggctg aaatcactca
     5641 caacgatgga tacgctaaca acttggaaat gaaat
//
