LOCUS       sequence-390428-       11226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  eEF1A1 fluorescent reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11226)
  AUTHORS   Oltion K, Carelli JD, Yang T, See SK, Wang HY, Kampmann M, Taunton J
  TITLE     An E3 ligase network engages GCN1 to promote the degradation of 
            translation factors on stalled ribosomes.
  JOURNAL   Cell. 2023 Jan 19;186(2):346-362.e17. doi: 
            10.1016/j.cell.2022.12.025. Epub 2023 Jan 12.
  PUBMED    36638793
REFERENCE   2  (bases 1 to 11226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.12.025"; journalName: "Cell"; date: 
            "2023-01-19- 19"; volume: "186"; issue: "2"; pages: "346-362.e17"
COMMENT     Sequence Label: pLX304 Flag-mCherry-eEF1A1_IRES-GFP
FEATURES             Location/Qualifiers
     source          1..11226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..24,25..31)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    39..55
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     44..66
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     63..79
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     63..79
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     98..118
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        100..118
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        146..372
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             373..553
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    600..725
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1218..1451
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1636..1680
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1855..2355
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2274..2292
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2367..2762
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    2822..2939
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3056..3359
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3360..3563
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3514..3534
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    3586..3610
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3619..3642
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3649..4356
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3794..3812)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4062..4081
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(5771..5795)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(5832..6406,6407..6409,6410..6418)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5999..6016)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6226..6245
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6419..7138
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     CDS             7141..7182
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7224..7812
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7277..7297)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7695..7706)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7884..8117
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8195..8316
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8232..8251)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8286..8305
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8356..8491
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8511..8530)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8512..8530)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8540..8557)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8540..8556)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8549..8571)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8698..9153
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8785..8804)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8995..9016
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9179..9283
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9284..9352,9353..10144)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9502..9521)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10315..10903
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10804..10823
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11057..11074
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11191..11212
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
       61 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
      121 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac
      181 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
      241 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
      301 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
      361 cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
      421 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
      481 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
      541 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
      601 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
      661 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
      721 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
      781 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
      841 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
      901 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
      961 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     1021 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     1081 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     1141 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     1201 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     1261 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     1321 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     1381 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     1441 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     1501 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     1561 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     1621 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     1681 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     1741 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     1801 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccttgcgc
     1861 cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg
     1921 aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc
     1981 cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt
     2041 cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc
     2101 actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg
     2161 gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg
     2221 gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc
     2281 gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc
     2341 gacctctctc cccagggggt accaccatgg ccaagccttt gtctcaagaa gaatccaccc
     2401 tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg
     2461 ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta
     2521 ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca
     2581 acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac
     2641 ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg
     2701 atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg
     2761 gcctgcagct gcagtagtaa gaattctaga tcttgagaca aatggcagta ttcatccaca
     2821 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     2881 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     2941 gggtttatta cagggacagc agagatccac tttggcgccg gctcgagggg gcccggggtt
     3001 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     3061 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     3121 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     3181 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     3241 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     3301 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     3361 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     3421 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     3481 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     3541 gggaggtcta tataagcaga gctctctggc taactgtcgg gatcaacaag tttgtacaaa
     3601 aaagcaggct tcaccatgga ctacaaagac gatgacgaca agctcgagat ggtgagcaag
     3661 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     3721 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     3781 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     3841 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     3901 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     3961 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     4021 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     4081 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     4141 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     4201 acctacaagg ccaagaagcc cgtgcagcta cccggcgcct acaacgtcaa catcaagttg
     4261 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     4321 cgccactcca ccggcggcat ggacgagctg tacaagggtg gctctggagg gactggtggc
     4381 atgggaaagg aaaagactca tatcaacatt gtcgtcattg gacacgtaga ttcgggcaag
     4441 tccaccacta ctggccatct gatctataaa tgcggtggca tcgacaaaag aaccattgaa
     4501 aaatttgaga aggaggctgc tgagatggga aagggctcct tcaagtatgc ctgggtcttg
     4561 gataaactga aagctgagcg tgaacgtggt atcaccattg atatctcctt gtggaaattt
     4621 gagaccagca agtactatgt gactatcatt gatgccccag gacacagaga ctttatcaaa
     4681 aacatgatta cagggacatc tcaggctgac tgtgctgtcc tgattgttgc tgctggtgtt
     4741 ggtgaatttg aagctggtat ctccaagaat gggcagaccc gagagcatgc ccttctggct
     4801 tacacactgg gtgtgaaaca actaattgtc ggtgttaaca aaatggattc cactgagcca
     4861 ccctacagcc agaagagata tgaggaaatt gttaaggaag tcagcactta cattaagaaa
     4921 attggctaca accccgacac agtagcattt gtgccaattt ctggttggaa tggtgacaac
     4981 atgctggagc caagtgctaa catgccttgg ttcaagggat ggaaagtcac ccgtaaggat
     5041 ggcaatgcca gtggaaccac gctgcttgag gctctggact gcatcctacc accaactcgt
     5101 ccaactgaca agcccttgcg cctgcctctc caggatgtct acaaaattgg tggtattggt
     5161 actgttcctg ttggccgagt ggagactggt gttctcaaac ccggtatggt ggtcaccttt
     5221 gctccagtca acgttacaac ggaagtaaaa tctgtcgaaa tgcaccatga agctttgagt
     5281 gaagctcttc ctggggacaa tgtgggcttc aatgtcaaga atgtgtctgt caaggatgtt
     5341 cgtcgtggca acgttgctgg tgacagcaaa aatgacccac caatggaagc agctggcttc
     5401 actgctcagg tgattatcct gaaccatcca ggccaaataa gcgccggcta tgcccctgta
     5461 ttggattgcc acacggctca cattgcatgc aagtttgctg agctgaagga aaagattgat
     5521 cgccgttctg gtaaaaagct ggaagatggc cctaaattct tgaagtctgg tgatgctgcc
     5581 attgttgata tggttcctgg caagcccatg tgtgttgaga gcttctcaga ctatccacct
     5641 ttgggtcgct ttgctgttcg tgatatgaga cagacagttg cggtgggtgt catcaaagca
     5701 gtggacaaga aggctgctgg agctggcaag gtcaccaagt ctgcccagaa agctcagaag
     5761 gctaaatgag acccagcttt cttgtacaaa gtgggaattc gcagtcgacg gtaccgcggg
     5821 cccgggatcc gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa
     5881 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     5941 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     6001 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     6061 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     6121 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     6181 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     6241 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     6301 gggcctcggt acacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     6361 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg ccacaaccat
     6421 ggtgagcaag ggcgccgagc tgttcaccgg catcgtgccc atcctgatcg agctgaatgg
     6481 cgatgtgaat ggccacaagt tcagcgtgag cggcgagggc gagggcgatg ccacctacgg
     6541 caagctgacc ctgaagttca tctgcaccac cggcaagctg cctgtgccct ggcccaccct
     6601 ggtgaccacc ctgagctacg gcgtgcagtg cttctcacgc taccccgatc acatgaagca
     6661 gcacgacttc ttcaagagcg ccatgcctga gggctacatc caggagcgca ccatcttctt
     6721 cgaggatgac ggcaactaca agtcgcgcgc cgaggtgaag ttcgagggcg ataccctggt
     6781 gaatcgcatc gagctgaccg gcaccgattt caaggaggat ggcaacatcc tgggcaataa
     6841 gatggagtac aactacaacg cccacaatgt gtacatcatg accgacaagg ccaagaatgg
     6901 catcaaggtg aacttcaaga tccgccacaa catcgaggat ggcagcgtgc agctggccga
     6961 ccactaccag cagaataccc ccatcggcga tggccctgtg ctgctgcccg ataaccacta
     7021 cctgtccacc cagagcgccc tgtccaagga ccccaacgag aagcgcgatc acatgatcta
     7081 cttcggcttc gtgaccgccg ccgccatcac ccacggcatg gatgagctgt acaagtgatt
     7141 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct
     7201 aaccggtggc gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga
     7261 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     7321 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     7381 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     7441 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     7501 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     7561 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     7621 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     7681 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     7741 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     7801 ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca
     7861 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     7921 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7981 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     8041 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     8101 gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata
     8161 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     8221 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     8281 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     8341 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     8401 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     8461 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     8521 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     8581 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     8641 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     8701 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     8761 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     8821 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     8881 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     8941 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     9001 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     9061 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     9121 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     9181 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     9241 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     9301 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     9361 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     9421 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     9481 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     9541 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     9601 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     9661 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     9721 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     9781 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     9841 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     9901 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     9961 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
    10021 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
    10081 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
    10141 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
    10201 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
    10261 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
    10321 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
    10381 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
    10441 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
    10501 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
    10561 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
    10621 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
    10681 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
    10741 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
    10801 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
    10861 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
    10921 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
    10981 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
    11041 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
    11101 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
    11161 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
    11221 ccaggc
//
