LOCUS       sequence-390585-        2054 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 module 6 with Esp3I expansion point.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pJMC-6-Esp3I
FEATURES             Location/Qualifiers
     source          1..2054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        135..239
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1308..1896
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1797..1816
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2050..2054,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc acctgctttt cggaaccgtg agacgaacaa
       61 caacgtctct agtgctgctt ttgcaggtgt gatgaaaagc cttagggtca ggtggcactt
      121 ttcggggaaa tgtgcgatga acccctattt gtttattttt ctaaatacat tcaaatatgt
      181 atccgctaat gagacaataa ccctgataaa tgcttcaatt atattgaaaa aggaagagta
      241 tgagtatcca acatttccgt gtcgccctta ttcccttttt ttgcggcctt ctgccttcct
      301 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgcc
      361 cgagtgggtt acattgaact ggatctcaac agcggcaaaa tcttagagag ttttcgcccc
      421 gaagaacgct tcccaatgat gagcactttc aaagttctgc tatgtggcgc ggtgttatcc
      481 cgtattgatg ctgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
      541 gttgagtatt caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
      601 tgtagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatt
      661 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
      721 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
      781 cctgtagcaa tggcaacaac tctccgcaaa ctattaactg gcgaactact tactcttgct
      841 tcacgccagc aactcattga ctggatggag gcggacaaag ttgcaggacc acttctgcgc
      901 tcggcacttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtggctct
      961 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     1021 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     1081 tcactgatta agcattggta actgtcagac gaagggaaat aaatagtagc ccgcctgatg
     1141 tgcgggcttt tttttggtgg tggttaccag tgtcgcttag ctaccgtagg tcctcttagg
     1201 cggtccgatt agcgacatag tcatcaatgc cttgacggca aaatccctta acgtgagttt
     1261 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     1321 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1381 ttgccggatc aagagctact aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1441 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     1501 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     1561 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     1621 ggctgaacgg ggggttcgtg ctcacagccc agcttggagc gaacgaccta caccgaactg
     1681 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     1741 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     1801 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     1861 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     1921 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     1981 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     2041 accgagcgca gcga
//
