LOCUS       sequence-391015-        3255 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-1-EXOB-11
FEATURES             Location/Qualifiers
     source          1..3255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1135..1153)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1221..1325
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1547..2338
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2509..3097
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2998..3017
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3251..3255,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag gagaattcaa acagggttct ggcgtcgttc
      121 tcgtactgtt ttccccaggc cagtgcttta gcgttaactt ccggagccac accggtgcaa
      181 acctcagcaa gcagggtgtg gaagtaggac attttcatgt caggccactt ctttccggag
      241 cggggttttg ctatcacgtt gtgaacttct gaagcggtga tgacgccgag ccgtaatttg
      301 tgccacgcat catccccctg ttcgacagct ctcacatcga tcccggtacg ctgcaggata
      361 atgtccggtg tcatgctgcc accttctgct ctgcggcttt ctgtttcagg aatccaagag
      421 cttttactgc ttcggcctgt gtcagttctg acgatgcacg aatgtcgcgg cgaaatatct
      481 gggaacagag cggcaataag tcgtcatccc atgttttatc cagggcgatc agcagagtgt
      541 taatctcctg catggtttca tcgttaaccg gagtgatgtc gcgttccggc tgacgttctg
      601 cagtgtatgc agtattttcg acaatgcgct cggcttcatc cttgtcatag ataccagcaa
      661 atccgaaggc cagacgggca cactgaatca tggctttatg acgtaacatc cgtttgggat
      721 gcgactgcca cggccccgtg atttctctgc cttcgcgagt tttgaatggt tcgcggcggc
      781 attcatccat ccattcggta acgcagatcg gatgattacg gtccttgcgg taaatccggc
      841 atgtacagga ttcattgtcc tgctcaaagt ccatgccatc aaactgctgg ttttcattga
      901 tgatgcggga ccagccatca acgcccacca ccggaacgat gccattctgc ttatcaggaa
      961 aggcgtaaat ttctttcgtc cacggattaa ggccgtactg gttggcaacg atcagtaatg
     1021 cgatgaactg cgcatcgctg gcatcacctt taaatgccgt ctggcgaaga gtggtgatca
     1081 gttcctgtgg gtcgacgctt gagaccacga agtggctctt cagtggacga aagggcctcg
     1141 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     1201 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     1261 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     1321 agagtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     1381 cagtggcggt tttcatggct tgttatgact gttttttttt ggggtacagt ctatgcctcg
     1441 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     1501 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatga gggaagcggt
     1561 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     1621 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     1681 cagcgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     1741 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     1801 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     1861 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     1921 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     1981 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     2041 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     2101 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     2161 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     2221 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
     2281 gttggaagaa tttgtccatt acgtaaaagg cgagatcacc aaggtagtcg gcaaataact
     2341 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     2401 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     2461 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     2521 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     2581 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     2641 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     2701 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     2761 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     2821 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     2881 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     2941 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     3001 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     3061 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     3121 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     3181 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     3241 gaccgagcgc agcga
//
