LOCUS       sequence-391019-        3041 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-1-Ugi4-11
FEATURES             Location/Qualifiers
     source          1..3041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(921..939)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1007..1111
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1333..2124
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2295..2883
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2784..2803
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3037..3041,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag gagcatagca aacaggctcc tggcttgcgt
      121 gtcccttaaa gcgcatccat gtgacaaagg atcctctgta atccgaggca acgatcgtgc
      181 agctgaaatg aattgagtta actatgcaat gttgctttta ggtaacttgg ctatgaatgc
      241 aaatcggctg gtgcagtggt gaatgagctg cggccgtggc ttcattccac tccaaatccc
      301 tccggtcccc atatccatta ttttggcagc ttttggttgc ctttaatgca agctttgggc
      361 gacagccatt caccacctca tttgtgctgc tcgggccacg gctgtattta gagaaattta
      421 acattttttt cataaaaaat gattactttt gtttcaaaaa attcaaaaat tcaggaatac
      481 ttaattttct aaaactgtag actcttatat tttatttttt actttcactt tgttgataaa
      541 gtaagccaaa tacgacccaa atatctgatc agtgaaagag taataatcac ctcaatacat
      601 gattggtgaa aaagatttaa ctgataggcc atttagaaat ctgactatga aaccagattt
      661 gtaaatgacc cattgattga gaggtttatg ggcaggatag tttctttgaa gatctctggc
      721 acagcagtta atctcatgca ctgaggtaag gcttgatatc atagaactgg attatgaaaa
      781 ccaatgcatg tgatatgaaa gcaatgagga catttcactt caactattat tcatgtagtc
      841 atcaggcacc aatggctaga gactacaaaa agaatcaact acgcttgaga ccacgaagtg
      901 gctcttcagt ggacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
      961 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     1021 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     1081 aatgcttcaa taatattgaa aaaggaagag tatgcgctca cgcaactggt ccagaacctt
     1141 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     1201 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     1261 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     1321 aagttaaaca tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta
     1381 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
     1441 gcagtggatg gcggcctgaa gccacacagc gatattgatt tgctggttac ggtgaccgta
     1501 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
     1561 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
     1621 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
     1681 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
     1741 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
     1801 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg
     1861 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc
     1921 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg
     1981 ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa
     2041 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccattacgt aaaaggcgag
     2101 atcaccaagg tagtcggcaa ataactgtca gaccaagttt actcatatat actttagatt
     2161 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     2221 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     2281 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2341 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2401 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     2461 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2521 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2581 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2641 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2701 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2761 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2821 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2881 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2941 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     3001 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg a
//
