LOCUS       sequence-391031-        3366 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-1-NtEf1a-6
FEATURES             Location/Qualifiers
     source          1..3366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1246..1264)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1332..1436
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1658..2449
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2620..3208
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3109..3128
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3362..3366,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag gaggtaccgg ccttagttaa agtgtcaaaa
      121 tggaaactgg agccaagttt aaggggccgg acaagttggc cttagttaaa tctaattcag
      181 tgggggccca agaagcccaa cccgggtacg atttctgagc tttaaaaaca tcactcggtg
      241 catttctaaa accctaacgc ttcgcttcat ttattctgcg cagccagggt tttagcgaag
      301 ctctctcgtc ttttcctttc gtctttttaa ggttagttct gttctatttt tctgcctatt
      361 atttatatat attcatacaa atcaattgta ttctagttgg tgttttgtca gtgatttgtt
      421 gaaaaacaca atctaattac cttgtgaatt gattttactt gagtttgaat tatgagattt
      481 atttgttttt atttacgaat taattgatat cataactttt tcttcccttt tttaatctcg
      541 tctatgtggt attgtccttt tgtttttgta tattagattt caactgttct actaattggt
      601 tacagcctat acgatttatt tttttatttg ttaattcgat ttgatttgct acacttagat
      661 tttgatctat tgatggtttt ttttaaaatg gatttttatg atatgtacac ttgaagtcaa
      721 ttgaataatg aatctacgac tattagaaac tctatagact ctggtatgtt tggtatgata
      781 gaaaatgttt tcctttttat tccgtgtttg atttgtaact gaactttttg ctaagaattt
      841 gtgaaagatt gatagtatcg ttttgatgaa aatttataga tgtccagatt gatgataatg
      901 gctgattgtt gaaaaggaaa atgaattctg tctgtggaag ttacattcct ggaacatctt
      961 ttccttggaa atgtttttgg ttaatcaaac accaaaagat aaatttcttc gtagtccctc
     1021 attccacccc caaaatttac gtattacatg gtctagagtt aagtttcaca tatttcaaag
     1081 agaaccatta gacaagagtc taattgaaag tttgtttctt tgtagaaatt attttggttt
     1141 caatgactat caaaatttaa ttcttggttt tctgttcttg tgatgatctt cagtttttaa
     1201 ggaatcaatg tgagaccacg aagtggctct tcagtggacg aaagggcctc gtgatacgcc
     1261 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     1321 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     1381 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatgc
     1441 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
     1501 ttttcatggc ttgttatgac tgtttttttt tggggtacag tctatgcctc gggcatccaa
     1561 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     1621 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga
     1681 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     1741 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagcgatat
     1801 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     1861 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     1921 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     1981 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     2041 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     2101 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     2161 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     2221 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     2281 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     2341 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     2401 atttgtccat tacgtaaaag gcgagatcac caaggtagtc ggcaaataac tgtcagacca
     2461 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     2521 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     2581 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2641 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     2701 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     2761 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     2821 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     2881 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2941 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     3001 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     3061 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     3121 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     3181 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     3241 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     3301 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     3361 cagcga
//
