LOCUS       sequence-391047-        9811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-SWAT compatible vector for C-terminal tagging with IAA17 
            (Auxin-Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 has SIR4 
            homology arms for genome integration and does not have NLS tags...
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Shively CA, Dong F, Mitra RD
  TITLE     A Suite of New Strain Construction Vectors for Gene Expression 
            Knockdown in Budding Yeast.
  JOURNAL   ACS Synth Biol. 2023 Jan 17. doi: 10.1021/acssynbio.2c00547.
  PUBMED    36650116
REFERENCE   2  (bases 1 to 9811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Jan 17. doi: 10.1021/acssynbio.2c00547."
COMMENT     Sequence Label: C-SWAT IAA17-6FLAG (no NLS)
FEATURES             Location/Qualifiers
     source          1..9811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             440..1774
                     /codon_start=1
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /product="O-acetylserine and O-acetylhomoserine 
                     sulfhydrylase, required for sulfur amino acid synthesis"
                     /label=MET17
                     /translation="MPSHFDTVQLHAGQENPGDNAHRSRAVPIYATTSYVFENSKHGSQ
                     LFGLEVPGYVYSRFQNPTSNVLEERIAALEGGAAALAVSSGQAAQTLAIQGLAHTGDNI
                     VSTSYLYGGTYNQFKISFKRFGIEARFVEGDNPEEFEKVFDERTKAVYLETIGNPKYNV
                     PDFEKIVAIAHKHGIPVVVDNTFGAGGYFCQPIKYGADIVTHSATKWIGGHGTTIGGII
                     VDSGKFPWKDYPEKFPQFSQPAEGYHGTIYNEAYGNLAYIVHVRTELLRDLGPLMNPFA
                     SFLLLQGVETLSLRAERHGENALKLAKWLEQSPYVSWVSYPGLASHSHHENAKKYLSNG
                     FGGVLSFGVKDLPNADKETDPFELSGAQVVDNLKLASNLANVGDAKTLVIAPYFTTHKQ
                     LNDKEKLASGVTKDLIRVSVGIEFIDDIIADFQQSFETVFAGQKP"
     rep_origin      complement(1879..2334)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2016..2037)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2228..2247
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2460..2482
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2474..2491
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2475..2491
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2501..2520
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2501..2519
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2544..2560
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2545..2561
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2655..3335
                     /codon_start=1
                     /gene="Arabidopsis thaliana AXR3"
                     /product="auxin-responsive IAA17 protein"
                     /label=auxin-inducible degron
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="GSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLNNE
                     PANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGG
                     PEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFMNE
                     RKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAMEK
                     CKSRA"
     CDS             3381..3404
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3411..3434
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3441..3464
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3471..3494
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3501..3524
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3531..3554
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        4042..4443
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             4996..5022
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5032..5088
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5122..6843
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="TYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFVG
                     NCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEEL
                     RMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEVE
                     DRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTLA
                     KILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYPL
                     CAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELRV
                     FPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRLC
                     ILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSIA
                     FAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKGC
                     QVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     primer_bind     complement(7038..7058)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7038..7056)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7077..7093)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7077..7093)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7090..7112)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7101..7117
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7125..7131,7132..7149,7150..7155))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7170..7191
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7308..7325)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7479..8067)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7559..7578)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8238..9029,9030..9098))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8861..8880
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9099..9203)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    9240..9743
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     9784..9802
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accacgcttt tcaattcaat tcatcaccat cctcatgaaa actgtgtaac ataataaccg
      241 aagtgtcgaa aaggtggcac cttgtccaat tgaacacgct cgatgaaaaa aataagatat
      301 atataaggtt aagtaaagcg tctgttagaa aggaagtttt tcctttttct tgctctcttg
      361 tcttttcatc tactatttcc ttcgtgtaat acagggtcgt cagatacata gatacaattc
      421 tattaccccc atccatacaa tgccatctca tttcgatact gttcaactac acgccggcca
      481 agagaaccct ggtgacaatg ctcacagatc cagagctgta ccaatttacg ccaccacttc
      541 ttatgttttc gaaaactcta agcatggttc gcaattgttt ggtctagaag ttccaggtta
      601 cgtctattcc cgtttccaaa acccaaccag taatgttttg gaagaaagaa ttgctgcctt
      661 agaaggtggt gctgctgctt tggctgtttc ctccggtcaa gccgctcaaa cccttgccat
      721 ccaaggtttg gcacacactg gtgacaacat cgtttccact tcttacttat acggtggtac
      781 ttataaccag ttcaaaatct cgttcaaaag atttggtatc gaggctagat ttgttgaagg
      841 tgacaatcca gaagaattcg aaaaggtctt tgatgaaaga accaaggctg tttatttgga
      901 aaccattggt aatccaaagt acaatgttcc ggattttgaa aaaattgttg caattgctca
      961 caaacacggt attccagttg tcgttgacaa cacatttggt gccggtggtt acttctgtca
     1021 gccaattaaa tacggtgctg atattgtaac acattctgct accaaatgga ttggtggtca
     1081 tggtactact atcggtggta ttattgttga ctctggtaag ttcccatgga aggactaccc
     1141 agaaaagttc cctcaattct ctcaacctgc cgaaggatat cacggtacta tctacaatga
     1201 agcctacggt aacttggcat acatcgttca tgttagaact gaactattaa gagatttggg
     1261 tccattgatg aacccatttg cctctttctt gctactacaa ggtgttgaaa cattatcttt
     1321 gagagctgaa agacacggtg aaaatgcatt gaagttagcc aaatggttag aacaatcccc
     1381 atacgtatct tgggtttcat accctggttt agcatctcat tctcatcatg aaaatgctaa
     1441 gaagtatcta tctaacggtt tcggtggtgt cttatctttc ggtgtaaaag acttaccaaa
     1501 tgccgacaag gaaactgacc cattcgaact ttctggtgct caagttgttg acaatttaaa
     1561 gcttgcctct aacttggcca atgttggtga tgccaagacc ttagtcattg ctccatactt
     1621 cactacccac aaacaattaa atgacaaaga aaagttggca tctggtgtta ccaaggactt
     1681 aattcgtgtc tctgttggta tcgaatttat tgatgacatt attgcagact tccagcaatc
     1741 ttttgaaact gttttcgctg gccaaaaacc atgagtgtgc gtgatgagtt gtaaaattat
     1801 gtataaacct actttctctc acaagtactg cggtgtgaaa taccgcacag atgcgtaagg
     1861 agaaaatacc gcatcaggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     1921 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     1981 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     2041 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     2101 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     2161 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     2221 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     2281 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc
     2341 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     2401 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     2461 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg
     2521 cgaattgggt accgggcccc ccctcgaggt cgacggtatc gataagcttg atatcgaatt
     2581 cctgcagcta tattaccctg ttatccctac gtacgctgca ggtcgacggt ggcggttctg
     2641 gcggtggcgg atccggctct gttgagttaa acctgcgtga gaccgagcta tgtcttggtc
     2701 tacccggtgg tgacactgta gcaccagtga caggcaacaa aaggggcttc agcgagactg
     2761 tagatctgaa gctaaactta aacaacgaac cagctaataa ggaaggtagt acaacacatg
     2821 atgtggttac gtttgacagc aaggagaaga gtgcctgtcc caaagatccg gcaaagcccc
     2881 cggcgaaggc ccaggttgta ggttggcctc cggtaaggtc ctatcgtaaa aacgtaatgg
     2941 ttagttgtca gaagtccagc ggaggacctg aagcagcggc gttcgtcaaa gtatctatgg
     3001 atggcgcccc atacttgaga aagatagacc tgcgtatgta taagtcttac gacgagttat
     3061 caaatgcgct atctaacatg ttcagcagtt tcaccatggg caaacatggt ggtgaggagg
     3121 gaatgatcga cttcatgaac gagagaaaac ttatggacct ggtgaatagc tgggattatg
     3181 tccctagcta cgaggataaa gacggggatt ggatgcttgt gggggatgtt ccgtggccga
     3241 tgttcgtcga tacgtgtaag cgtctgcgtc taatgaaggg ctccgatgct attggtttag
     3301 ctccgcgtgc tatggaaaag tgtaagtcca gagctgtatc acccgggata cattatacga
     3361 agttatgcat gctcactagt gactacaagg acgacgatga caagtctagt gactacaagg
     3421 acgacgatga caagtctagt gactacaagg acgacgatga caagtctagt gactacaagg
     3481 acgacgatga caagtctagt gactacaagg acgacgatga caagtctagt gactacaagg
     3541 acgacgatga caagtctaga tgaggcgcgc tagatctgtt tagcttaaga gttcttcttt
     3601 gagatatcga ttgaacgagc tcgaattcat cgatctatat taccctgtta tccctagcgt
     3661 aactatgcca aatgacaata agacacccaa taggtccagc actcccaagt ttactaaaaa
     3721 accagtaacc ccgaatgata aaattcctga aagacgggtg tacaatatgg acttcctctt
     3781 ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt ctccctaaca
     3841 tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca taatgggcta
     3901 aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa ctaatactgt
     3961 agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt ccatttgcca
     4021 tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt ttctctctcc
     4081 cccgttgttg tctcaccata tccgcaatga caaaaaaatg atggaagaca ctaaaggaaa
     4141 aaattaacga caaagacagc accaacagat gtcgttgttc cagagctgat gaggggtatc
     4201 tcgaagcaca cgaaactttt tccttccttc attcacgcac actactctct aatgagcaac
     4261 ggtatacggc cttccttcca gttacttgaa tttgaaataa aaaaaagttt gctgtcttgc
     4321 tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat tgttctcgtt
     4381 ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag catacaatca
     4441 actatctcat atacaactag aatgaccctt acgagcggtc aacgtgacca cagagacgtt
     4501 gaaaatgagt tatccagtgc agcttttagc agtcaaagtt tcggggttcc agatatgtca
     4561 ttcaaaccgg ggtgctccag tgacgtcgca gtcactgatg cgggtatgcc tagccaaggg
     4621 ttatggaaca accagacgca aaggatgcgt accttcacta aggtccagaa aaggggctct
     4681 gttgggagga gtatcgacat caccaggtat cgtgattacg atgagctacg tcatgatctg
     4741 gcatgtatgt tcggcataca ggggcagtta gaagaccctt atcgtatgga ttggaagtta
     4801 gtttatgtgg atcacgagaa cgatatactg ttggtgggcg acgacccgtg ggaggaattt
     4861 gtaggttgtg ttaagtctat caagattcta tctgctgcag aggtgcagca aatgagcttg
     4921 gacggggatc ttggtggagt tccgccccaa acccaggcrt gcagtgcttc agacgatgcg
     4981 aacgcttgga gaggctatcc ctacgacgtg cctgattatg cgggtagcgg ggccactaac
     5041 ttcagcttgt tgaagcaggc tggcgatgtg gaggagaatc caggtccggc ggctaccgat
     5101 aaagcggaat taattcccga gacgtacttc ccggaggagg tggtggagca catcttcagc
     5161 ttcctgccgg cgcagcgcga ccgcaacacg gtctcgctcg tctgcaaggt gtggtacgag
     5221 atcgagaggc tgagccgccg cggcgtcttc gtgggcaact gctacgccgt gcgcgccggc
     5281 cgcgtcgccg cgcggttccc caacgtgcgg gcgctcacgg tgaaggggaa gccccacttc
     5341 gccgacttca acctcgtgcc ccccgactgg ggcggctacg cggggccgtg gatcgaggcg
     5401 gccgcgaggg gatgccacgg cctggaggag ctcaggatga agcggatggt ggtgtccgac
     5461 gagagcctcg agctgctggc tcgctcgttc ccgcggttca gggctcttgt tcttatcagc
     5521 tgcgaggggt tcagcactga cgggctagcc gccgtcgcga gccattgcaa gcttctgagg
     5581 gagttggatt tgcaggaaaa tgaagtggag gatcgagggc ctaggtggct ttcctgcttc
     5641 cctgattcct gcacatcact tgtctcattg aattttgcct gcatcaaagg ggaggttaat
     5701 gctggttcac tggagagact tgttagcagg tccccaaacc tgcggagttt gaggctgaat
     5761 cgatctgtat cggtagatac acttgcaaag atactactgc gtacccctaa cttggaggat
     5821 ttggggacag ggaatttgac agatgacttc caaactgagt cctactttaa gcttaccagt
     5881 gctctggaga aatgcaagat gttgaggagt ttgtctggat tctgggatgc ttctcctgtt
     5941 tgcctgtcat ttatctaccc cctgtgtgct caactgacag gattgaactt gagctatgca
     6001 cccacacttg atgcttctga ccttacaaaa atgattagcc gctgtgtgaa gctccaacgc
     6061 ctttgggtac tggattgtat ctcggacaaa ggcttgcaag tggtggcctc cagttgcaaa
     6121 gacttgcaag aactcagggt atttccatca gatttctacg tagctggtta ttctgcagtg
     6181 acagaggagg gacttgttgc agtatccttg ggctgtccaa aactgaactc actactgtac
     6241 ttctgtcacc aaatgactaa tgctgcacta gttactgtcg ccaagaactg tccaaatttc
     6301 acacgattca gactttgtat tcttgagcca gggaagcctg atgttgtgac aagccaacca
     6361 ttagatgaag gctttggagc tattgttcgt gagtgcaagg gattacaacg tttgtcaata
     6421 tctggtcttc tcacagacaa agttttcatg tatattggga aatatgcaaa acaacttgag
     6481 atgctttcta tagcatttgc tggtgacagt gataagggta tgatgcatgt tatgaatgga
     6541 tgcaagaatt taaggaaact ggagataaga gatagcccgt ttggtgatgc tgcactcttg
     6601 gggaattttg ctaggtacga gacaatgcga tccctttgga tgtcatcttg caatgtcacg
     6661 ttaaaggggt gccaagtcct tgcgtcaaag atgccgatgc tcaatgttga ggtcataaat
     6721 gagcgggatg gtagcaatga aatggaggaa aaccatggag atctgcctaa agtggagaaa
     6781 ttatatgtgt accgcacaac tgctggggcg agggatgatg caccaaattt tgttaaaatc
     6841 ctagtcgact ccggttctgc tgctagtggt tgaacgcgtg gcgcctggaa aaagattttc
     6901 aagtgaataa ggagataaaa ccgtattgac tttttatcat tctacaaaag accagtaacg
     6961 aagtgtaccc tgctatatta ccctgttatc cctagcgtaa ctcggccgcc accgcggtgg
     7021 agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
     7081 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     7141 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     7201 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     7261 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     7321 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     7381 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     7441 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     7501 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     7561 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     7621 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     7681 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7741 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7801 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7861 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     7921 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7981 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     8041 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     8101 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     8161 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     8221 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     8281 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     8341 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     8401 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     8461 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     8521 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     8581 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     8641 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     8701 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     8761 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     8821 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     8881 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     8941 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9001 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9061 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     9121 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     9181 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtccttttca
     9241 tcacgtgcta taaaaataat tataatttaa attttttaat ataaatatat aaattaaaaa
     9301 tagaaagtaa aaaaagaaat taaagaaaaa atagtttttg ttttccgaag atgtaaaaga
     9361 ctctaggggg atcgccaaca aatactacct tttatcttgc tcttcctgct ctcaggtatt
     9421 aatgccgaat tgtttcatct tgtctgtgta gaagaccaca cacgaaaatc ctgtgatttt
     9481 acattttact tatcgttaat cgaatgtata tctatttaat ctgcttttct tgtctaataa
     9541 atatatatgt aaagtacgct ttttgttgaa attttttaaa cctttgttta tttttttttc
     9601 ttcattccgt aactcttcta ccttctttat ttactttcta aaatccaaat acaaaacata
     9661 aaaataaata aacacagagt aaattcccaa attattccat cattaaaaga tacgaggcgc
     9721 gtgtaagtta caggcaagcg atccgtctaa gaaaccatta ttatcatgac attaacctat
     9781 aaaaataggc gtatcacgag gccctttcgt c
//
