LOCUS       sequence-391049-        8913 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses helicase dead DDX5 cloned into pLenti puro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8913)
  AUTHORS   McCann JJ, Fleenor DE, Chen J, Lai CH, Bass TE, Kastan MB
  TITLE     Participation of ATM, SMG1, and DDX5 in a DNA Damage-Induced 
            Alternative Splicing Pathway.
  JOURNAL   Radiat Res. 2023 Apr 1;199(4):406-421. doi: 10.1667/RADE-22-00219.1.
  PUBMED    36921295
REFERENCE   2  (bases 1 to 8913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1667/RADE-22-00219"; journalName: "Radiat Res"; date: 
            "2023-04-1- 1"; volume: "199"; issue: "4"; pages: "406-421"
COMMENT     Sequence Label: DDX5 D248N V5
FEATURES             Location/Qualifiers
     source          1..8913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..111
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    228..249
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(264..269,270..287,288..294)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    302..318
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     307..329
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     326..342
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     326..342
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     361..381
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        363..381
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        409..635
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             636..816
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    863..988
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1481..1714
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1899..1943
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2525..2728
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2679..2699
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2730..2748
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2751..2769
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             4705..4746
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4756..4773
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4796..4813)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(4825..4845)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4830..5159
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5010..5145
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5169..5768
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5169..5188)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5665..5685
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5840..6073
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6145..6266
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6182..6201)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6236..6255
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6306..6441
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6461..6480)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6462..6480)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6490..6507)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6490..6506)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6499..6521)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6648..7103
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6735..6754)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6945..6966
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7129..7233
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7234..7302,7303..8094)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7452..7471)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8265..8853
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8754..8773
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      241 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      301 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
      361 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
      421 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
      481 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
      541 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
      601 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
      661 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
      721 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
      781 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
      841 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
      901 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
      961 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     1021 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     1081 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     1141 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     1201 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     1261 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     1321 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     1381 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     1441 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     1501 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     1561 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     1621 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     1681 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     1741 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     1801 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     1861 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     1921 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     1981 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     2041 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     2101 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     2161 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgataagct tgggagttcc
     2221 gcttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     2281 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     2341 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     2401 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     2461 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     2521 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     2581 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     2641 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     2701 acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc tccctatcag
     2761 tgatagagat cgtcgactag tggatccagt gtggtggaat tctgcagata tccagcacag
     2821 tggcggccgc tcgagtctag aatgtcgggt tattcgagtg accgagaccg cggccgggac
     2881 cgagggtttg gtgcacctcg atttggagga agtagggcag ggcccttatc tggaaagaag
     2941 tttggaaacc ctggggagaa attagttaaa aagaagtgga atcttgatga gctgcctaaa
     3001 tttgagaaga atttttatca agagcaccct gatttggcta ggcgcacagc acaagaggtg
     3061 gaaacataca gaagaagcaa ggaaattaca gttagaggtc acaactgccc gaagccagtt
     3121 ctaaattttt atgaagccaa tttccctgca aatgtcatgg atgttattgc aagacagaat
     3181 ttcactgaac ccactgctat tcaagctcag ggatggccag ttgctctaag tggattggat
     3241 atggttggag tggcacagac tggatctggg aaaacattgt cttatttgct tcctgccatt
     3301 gtccacatca atcatcagcc attcctagag agaggcgatg ggcctatttg tttggtgctg
     3361 gcaccaactc gggaactggc ccaacaggtg cagcaagtag ctgctgaata ttgtagagca
     3421 tgtcgcttga agtctacttg tatctacggt ggtgctccta agggaccaca aatacgtgat
     3481 ttggagagag gtgtggaaat ctgtattgca acacctggaa gactgattga ctttttagag
     3541 tgtggaaaaa ccaatctgag aagaacaacc taccttgtcc ttaatgaagc agatagaatg
     3601 cttgatatgg gctttgaacc ccaaataagg aagattgtgg atcaaataag acctgatagg
     3661 caaactctaa tgtggagtgc gacttggcca aaagaagtaa gacagcttgc tgaagatttc
     3721 ctgaaagact atattcatat aaacattggt gcacttgaac tgagtgcaaa ccacaacatt
     3781 cttcagattg tggatgtgtg tcatgacgta gaaaaggatg aaaaacttat tcgtctaatg
     3841 gaagagatca tgagtgagaa ggagaataaa accattgttt ttgtggaaac caaaagaaga
     3901 tgtgatgagc ttaccagaaa aatgaggaga gatgggtggc ctgccatggg tatccatggt
     3961 gacaagagtc aacaagagcg tgactgggtt ctaaatgaat tcaaacatgg aaaagctcct
     4021 attctgattg ctacagatgt ggcctccaga gggctagatg tggaagatgt gaaatttgtc
     4081 atcaattatg actaccctaa ctcctcagag gattatattc atcgaattgg aagaactgct
     4141 cgcagtacca aaacaggcac agcatacact ttctttacac ctaataacat aaagcaagtg
     4201 agcgacctta tctctgtgct tcgtgaagct aatcaagcaa ttaatcccaa gttgcttcag
     4261 ttggtcgaag acagaggttc aggtcgttcc aggggtagag gaggcatgaa ggatgaccgt
     4321 cgggacagat actctgcggg caaaaggggt ggatttaata cctttagaga cagggaaaat
     4381 tatgacagag gttactctag cctgcttaaa agagattttg gggcaaaaac tcagaatggt
     4441 gtttacagtg ctgcaaatta caccaatggg agctttggaa gtaattttgt gtctgctggt
     4501 atacagacca gttttaggac tggtaatcca acagggactt accagaatgg ttatgatagc
     4561 actcagcaat acggaagtaa tgttccaaat atgcacaatg gtatgaacca acaggcatat
     4621 gcatatcctg ctactgcagc tgcacctatg attggttatc caatgccaac aggatattcc
     4681 caactagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat
     4741 tctacgcgta ccggtcatca tcaccatcac cattgagttt aaacctgctg atcagcctcg
     4801 actgtgcctt ctagtcgacc ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     4861 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     4921 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     4981 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     5041 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     5101 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     5161 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     5221 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     5281 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     5341 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     5401 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     5461 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     5521 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     5581 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     5641 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     5701 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     5761 gtgcctgagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     5821 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     5881 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     5941 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     6001 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     6061 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     6121 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     6181 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     6241 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     6301 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     6361 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     6421 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     6481 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     6541 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     6601 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     6661 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     6721 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     6781 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     6841 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     6901 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     6961 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     7021 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     7081 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     7141 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     7201 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     7261 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     7321 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     7381 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     7441 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     7501 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     7561 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     7621 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     7681 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     7741 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     7801 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     7861 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     7921 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     7981 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     8041 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     8101 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     8161 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     8221 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     8281 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     8341 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     8401 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     8461 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     8521 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     8581 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     8641 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     8701 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     8761 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     8821 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     8881 ttcctggcct tttgctggcc ttttgctcac atg
//
