LOCUS       sequence-391069-        4113 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-1-BeYDV-LRS-L-11
FEATURES             Location/Qualifiers
     source          1..4113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1993..2011)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2079..2183
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2405..3196
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3367..3955
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3856..3875
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(4109..4113,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag gagcaaatac catcacatcg tatatgcttt
      121 tatagtgtga acacctttaa ccctagtggg cgggaaattt tctactttaa atctggaccg
      181 ctcgtgctaa agcactcgcg ataagggggg gccacgccgg taatattaaa ttcggcgtgg
      241 gccccccctt gtcgcaaaga cttcgtcttt aagtaaattc cacgtcattt tccactatct
      301 attaaaatga ccaaaatacc cctgcctcca tgcctccacg ccggttataa gatagagttt
      361 gaggcaaccc ctcggagtca caacaacatg ccttctgcta gtaagaactt cagactccaa
      421 tctaaatatg ttttccttac ctatcccaag tgctcatctc aaagagatga tttattccag
      481 tttctctggg agaaactcac accttttctt attttcttcc ttggtgttgc ttctgagctt
      541 catcaagatg gcactaccca ctatcatgct ctgctccagc ttgataaaaa accttgtatt
      601 agggatcctt cttttttcga ttttgaagga aatcacccta atatccagcc agctagaaac
      661 tctaaacaag tccttgatta catatcaaag gacggagata ttaaaaccag aggagatttc
      721 cgagatcata aggtttctcc tcgcaaatct gacgcacgat ggagaactat tatccagact
      781 gcaacgtcta aggaggaata tcttgacatg atcaaggaag aattccctca tgaatgggca
      841 acaaagcttc aatggctgga atattcagcc aacaaattat tccctccaca acctgaaccg
      901 tatgtgtcgc ccttcacaga atcagatctt cgctgccacg aagatctaca ctcctggagg
      961 gaaacccatc tataccatgt aagcatagac gcttatactt acatacatcc tgtctcatac
     1021 caacaagctc aatctgacct tgaatggatg gccgatttaa caaggacaat ggaaggaatg
     1081 gaatccgaca ccccagcctc tacatctgcg gaccaactcg taccggaaag accacctggg
     1141 ctagaagtct cggacgacac aactattgga acggtaccat cgatttcacc aactacgatg
     1201 aacacgccac ctataatatc atcgacgaca tccccttcaa gttcgtccca ttgtggaagc
     1261 aattaatagg ttgccagtct gatttcactg tcaaccctaa atatggaaaa aagaagaaaa
     1321 taaaaggtgg gatcccttct ataattcttt gcaatcctga cgaggactgg atgttatcaa
     1381 tgacaagtca acagaaggat tactttaaag ataattgcgt cacccactac atgtgtgacg
     1441 gggagacttt ttttgctcgg gaatcgtcga gtcactgaac gtgcctctcc tcatacgagt
     1501 ttatctaaag tgattatttt ttttggggtg tttgtttttg gattgtcttt ttttgtttat
     1561 ttcgtgtgtt atgtaacata tgtaatttct atctacttgc acaatgaaat atattcataa
     1621 aataatcatt accgtgagac gaacaacaac gtctctagtg caaataccat cacatcgtat
     1681 atgcttttat agtgtgaaca cctttaaccc tagtgggcgg gaaattttct actttaaatc
     1741 tggaccgctc gtgctaaagc actcgcgata agggggggcc acgccggtaa tattaaattc
     1801 ggcgtgggcc cccccttgtc gcaaagactt cgtctttaag taaattccac gtcattttcc
     1861 actatctatt aaaatgacca aaatacccct gcctccatgc ctccacgccg gttataagat
     1921 agagtttgag gcaacccctc ggagtcacaa caacgcttga gaccacgaag tggctcttca
     1981 gtggacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     2041 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     2101 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     2161 aataatattg aaaaaggaag agtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     2221 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttttgg
     2281 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
     2341 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
     2401 catcatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
     2461 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
     2521 tggcggcctg aagccacaca gcgatattga tttgctggtt acggtgaccg taaggcttga
     2581 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
     2641 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
     2701 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
     2761 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
     2821 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
     2881 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
     2941 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     3001 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     3061 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
     3121 ggcctcgcgc gcagatcagt tggaagaatt tgtccattac gtaaaaggcg agatcaccaa
     3181 ggtagtcggc aaataactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     3241 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     3301 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     3361 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     3421 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     3481 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     3541 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3601 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3661 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     3721 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     3781 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     3841 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     3901 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     3961 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     4021 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     4081 cgctcgccgc agccgaacga ccgagcgcag cga
//
