LOCUS       sequence-391123-        2739 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-9-NtEU-11
FEATURES             Location/Qualifiers
     source          1..2739
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(619..637)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        705..809
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1031..1822
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1993..2581
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2482..2501
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2735..2739,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag cttaaagcag aatgctgagc taaaagaaag
      121 gctttttcca ttttcgagag acaatgagaa aagaagaaga agaagaagaa gaagaagaag
      181 aagaagaaga aaagagtaaa taataaagcc ccacaggagg cgaagttctt gtagctccat
      241 gttatctaag ttattgatat tgtttgccct atattttatt tctgtcattg tgtatgtttt
      301 gttcagtttc gatctccttg caaaatgcag agattatgag atgaataaac taagttatat
      361 tattatacgt gttaatattc tcctcctctc tctagctagc cttttgtttt ctctttttct
      421 tatttgattt tctttaaatc aatccatttt aggagagggc cagggagtga tccagcaaaa
      481 catgaagatt agaagaaact tccctctttt ttttcctgaa aacaatttaa cgtcgagatt
      541 tatctctttt tgtaatggaa tcatttctac agttatgacc gcttgagacc acgaagtggc
      601 tcttcagtgg acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
      661 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      721 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      781 tgcttcaata atattgaaaa aggaagagta tgcgctcacg caactggtcc agaaccttga
      841 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
      901 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
      961 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     1021 gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
     1081 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
     1141 agtggatggc ggcctgaagc cacacagcga tattgatttg ctggttacgg tgaccgtaag
     1201 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
     1261 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     1321 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     1381 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     1441 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     1501 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     1561 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     1621 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     1681 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     1741 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cattacgtaa aaggcgagat
     1801 caccaaggta gtcggcaaat aactgtcaga ccaagtttac tcatatatac tttagattga
     1861 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     1921 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1981 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2041 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2101 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     2161 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2221 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2281 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2341 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2401 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2461 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2521 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2581 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     2641 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     2701 tgataccgct cgccgcagcc gaacgaccga gcgcagcga
//
