LOCUS       sequence-391163-       10230 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of TRIM21-GFP fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10230)
  AUTHORS   Park JS, Burckhardt CJ, Lazcano R, Solis LM, Isogai T, Li L, Chen 
            CS, Gao B, Minna JD, Bachoo R, DeBerardinis RJ, Danuser G
  TITLE     Mechanical regulation of glycolysis via cytoskeleton architecture.
  JOURNAL   Nature. 2020 Feb 12. pii: 10.1038/s41586-020-1998-1. doi: 
            10.1038/s41586-020-1998-1.
  PUBMED    32051585
REFERENCE   2  (bases 1 to 10230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2020 Feb 12. pii: 10.1038/s41586-020-1998-1. doi: 
            10.1038/s41586-020-1998-1."
COMMENT     Sequence Label: pLVX puro TRIM21-GFP
FEATURES             Location/Qualifiers
     source          1..10230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             111..744
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    791..916
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1413..1646
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1831..1875
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2138..2253
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2311..2614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2615..2818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2769..2789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      4347..4356
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4353..4355,4356..4358,4359..5072)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4398..4419)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4659..4678)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5006..5027
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(5090..5664,5665..5667,5668..5676)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5257..5274)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5484..5503
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5696..6295
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5696..5715)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6192..6212
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6309..6897
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6362..6382)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6780..6791)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7104..7737
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7865..7881)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7865..7881)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7878..7900)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7889..7905
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7913..7919,7920..7937,7938..7943))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7958..7979
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8096..8113)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8267..8855)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8347..8366)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9026..9817,9818..9886))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9649..9668
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9887..9991)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10039..10173
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10076..10095)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10130..10149
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt
       61 tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt tggaagggct
      121 aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
      181 cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg
      241 atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga
      301 gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
      361 gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc
      421 ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt
      481 tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata
      541 agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
      601 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      661 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      721 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      781 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      841 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
      901 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
      961 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1021 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1081 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1141 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1201 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg
     1261 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     1321 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     1381 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     1441 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     1501 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     1561 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     1621 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     1681 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     1741 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     1801 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     1861 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     1921 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     1981 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     2041 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     2101 tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag gggggattgg
     2161 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     2221 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     2281 tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa atggcccgcc
     2341 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     2401 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     2461 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     2521 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     2581 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     2641 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     2701 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     2761 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg
     2821 tttagtgaac cgtcagatcc gctagcgcta ccggactcag atctcgagct caagcttcga
     2881 attctttcga gatggcttca gcagcacgct tgacaatgat gtgggaggag gtcacatgcc
     2941 ctatctgcct ggaccccttc gtggagcctg tgagcatcga gtgtggccac agcttctgcc
     3001 aggaatgcat ctctcaggtt gggaaaggtg ggggcagcgt ctgtcctgtg tgccggcagc
     3061 gctttctgct caagaatctc cggcccaatc gacagctagc caacatggtg aacaacctta
     3121 aagaaatcag ccaggaggcc agagagggca cacaggggga acggtgtgca gtgcatggag
     3181 agagacttca cctgttctgt gagaaagatg ggaaggccct ttgctgggta tgtgcccagt
     3241 ctcggaaaca ccgtgaccac gccatggtcc ctcttgagga ggctgcacag gagtaccagg
     3301 agaagctcca ggtggcatta ggggaactga gaagaaagca ggagttggct gagaagttgg
     3361 aagtggaaat tgcaataaag agagcagact ggaagaaaac agtggaaaca cagaaatcta
     3421 ggattcacgc agagtttgtg cagcaaaaaa acttcctggt tgaagaagaa cagaggcagc
     3481 tgcaggagct ggagaaggat gagagggagc agctgagaat cctgggggag aaagaggcca
     3541 agctggccca gcagagccag gccctacagg agctcatctc agagctagat cgaaggtgcc
     3601 acagctcagc actggaactg ctgcaggagg tgataattgt cctggaaagg agtgagtcct
     3661 ggaacctgaa ggacctggat attacctctc cagaactcag gagtgtgtgc catgtgccag
     3721 ggctgaagaa gatgctgagg acatgtgcag tccacatcac tctggatcca gacacagcca
     3781 atccgtggct gatactttca gaagatcgga gacaagtgag gcttggagac acccagcaga
     3841 gcatacctgg aaatgaagag agatttgata gttatcctat ggtcctgggt gcccagcact
     3901 ttcactctgg aaaacattac tgggaggtag atgtgacagg aaaggaggcc tgggacctgg
     3961 gtgtctgcag agactctgtg cgcaggaagg ggcacttttt gcttagttcc aagagtggct
     4021 tctggacaat ttggttgtgg aacaaacaaa aatatgaggc tggcacctac ccccagactc
     4081 ccctccacct tcaggtgcct ccatgccaag ttgggatttt cctggactat gaggctggca
     4141 tggtctcctt ctacaacatc actgaccatg gctccctcat ctactccttc tctgaatgtg
     4201 cctttacagg acctctgcgg cccttcttca gtcctggttt caatgatgga ggaaaaaaca
     4261 cagcccctct aaccctctgt ccactgaata ttggatcaca aggatccact gactatacgg
     4321 taccgcgggc ccgggatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca
     4381 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     4441 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     4501 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     4561 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     4621 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     4681 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     4741 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     4801 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     4861 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     4921 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca
     4981 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     5041 tcactctcgg catggacgag ctgtacaagt aaagcggccg cggatcccgc ccctctccct
     5101 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
     5161 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     5221 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     5281 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
     5341 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
     5401 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
     5461 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
     5521 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
     5581 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     5641 tcctttgaaa aacacgatga taagcttgcc acaacccaca aggagacgac cttccatgac
     5701 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac
     5761 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc cggaccgcca
     5821 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     5881 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     5941 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     6001 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     6061 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     6121 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     6181 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     6241 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctagacgcg
     6301 tctggaacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     6361 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     6421 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     6481 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     6541 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     6601 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     6661 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     6721 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     6781 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     6841 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     6901 gaattaattc tgcagtcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     6961 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
     7021 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     7081 ctttttaaaa gaaaagaggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     7141 ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga actacacacc
     7201 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     7261 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
     7321 cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct
     7381 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg
     7441 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
     7501 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt
     7561 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     7621 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     7681 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     7741 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     7801 gtgagaggcc ttgacattgc tagcgtttac cgtcgacctc tagctagagc ttggcgtaat
     7861 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     7921 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     7981 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     8041 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     8101 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     8161 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     8221 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8281 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8341 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8401 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8461 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8521 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8581 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8641 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8701 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8761 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8821 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8881 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     8941 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9001 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9061 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     9121 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9181 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9241 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9301 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     9361 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9421 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9481 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9541 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9601 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     9661 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     9721 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     9781 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     9841 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     9901 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     9961 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    10021 tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat
    10081 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    10141 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
    10201 catcccaaac ttcccacccc ataccctatt
//
