LOCUS       sequence-391220-       12971 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDEL157; transgenic construct to express cell-specific Cas9 in 
            sensory neurons; ubiquitous control sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12971)
  AUTHORS   Lysko DE, Talbot WS
  TITLE     Unmyelinated sensory neurons use Neuregulin signals to promote 
            myelination of interneurons in the CNS.
  JOURNAL   Cell Rep. 2022 Nov 15;41(7):111669. doi: 
            10.1016/j.celrep.2022.111669.
  PUBMED    36384112
REFERENCE   2  (bases 1 to 12971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2022-11-15"; volume: "41(7):111669. doi"; pages: " 
            10.1016/j.celrep.2022.111669"
COMMENT     Sequence Label: zCREST3:redCas9; control_gRNA
FEATURES             Location/Qualifiers
     source          1..12971
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        17..121
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(122..190,191..982)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(340..359)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1153..1741
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1642..1661
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1895..1912
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2029..2050
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2065..2070,2071..2088,2089..2095)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2103..2119
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2108..2130
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2127..2143
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2162..2182
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2164..2182
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2219..2235
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2219..2235
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(2269..2280,2281..2468)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     CDS             join(3690..3692,3693..3695,3696..4409)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3690..3692,3693..3695,3696..4406)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3735..3756)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3996..4015)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4343..4364
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4711..4845
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_RNA        5244..5319
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     5332..5348
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5356..5376
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    5800..5824
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(5843..5863)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(5879..9979)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes MGAS15252 Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFGSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLADSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQIYNQLFEENPINASRVDAKAILSARLSKSRRL
                     ENLIAQLPGEKRNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNSEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGAYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDRGMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGHSLHEQIANLAGSPAIKKGILQTVKIVDELVKVMGH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFIKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             5958..5966
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(9986..10006)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(10019..10072)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(10082..10810)
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="VSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTDM
                     ALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVA
                     RYCDLPSKLGHKLNGMDELYK"
     regulatory      10852..10861
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(10862..10886)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(11748..11768)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(11788..11804)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    join(12177..12339,12340..12351)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     12804..12825
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
       61 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      121 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      181 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
      241 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
      301 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
      361 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
      421 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
      481 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
      541 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
      601 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
      661 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
      721 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
      781 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
      841 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
      901 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
      961 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     1021 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     1081 gaccaaaatg ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1141 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1201 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     1261 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     1321 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     1381 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     1441 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     1501 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     1561 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     1621 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     1681 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     1741 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     1801 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     1861 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     1921 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     1981 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     2041 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     2101 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     2161 cgcaattaac cctcactaaa gggaacaaaa gctggagctc caccgcggtg gcggccgctc
     2221 tagaactagt ggatctgctg ggcttgctga aggtaggggg tcaagaacca gaggtgtaaa
     2281 gtacttgagt aattttactt gattactgta cttaagtatt atttttgggg atttttactt
     2341 tacttgagta caattaaaaa tcaatacttt tacttttact taattacatt tttttagaaa
     2401 aaaaagtact ttttactcct tacaatttta tttacagtca aaaagtactt attttttgga
     2461 gatcacttca ttctattttc ccttgctatt accaaaccaa ttgaattgcg ctgatgccca
     2521 gtttaattta aatgttattt attctgccta tgaaaatcgt tttcacatta tatgaaattg
     2581 gtcagacatg ttcattggtc ctttggaagt gacgtcatgt cacatctatt accacaatgc
     2641 acagcacctt gacctggaaa ttagggaaat tataacagtc aatcagtgga agaaaatgga
     2701 ggaagtatgt gattcatcag cagctgcgag cagcacagtc caaaatcagc cacaggatca
     2761 agagcacccg tggccgtatc ttcgcagatc taaagcttaa atcagttgtg ttaaataaga
     2821 gacattcaaa ataaatgtaa atgagctctc caaatcagca gacttaacat tctttaaaat
     2881 gattgattca atagtgataa aaatcaggca tagccagttg taactttaga taaattacag
     2941 aaaatgtcaa atacagagaa ccgattcttt tttatgatac atccaagcac acatttaaca
     3001 caatccaggc aaaccccgaa tttcacagtc acaagcactg tttgtacaag agctttgcct
     3061 aaggacacac agtctctata agtccaggtc gttggtttca ctcttatttt aaacatgtga
     3121 catttttcct gccatcctgt cttaggctgc tgtttgcttc attccatgtc acattaaatt
     3181 cctcagtagc accttttaca cacacagcca atcttttcca gaaaattcaa ttgctttgaa
     3241 gagataatgt gtgaacaaat ccatttagaa aaggaaaatt aagaatttgt aaaatcatct
     3301 gtaaattgtt ggcattcttc tgtatatgaa catcacatca tttacaggta aaggtctggt
     3361 cattaattat atgacaattt actggtatta ttttgtgaaa ggggctattt tcaatgcgtt
     3421 catccatcct tttcatccct caaatctctc attcacgtcc ccctccccat ctgcacactt
     3481 tatctcattt ccaccctgct ggaatctgag cacttgtgca gttatcaggg ctcctgtatt
     3541 taggaggctc tgggtgtcca tgtaggggac gaacagaaac actgcagacc tttatagaag
     3601 aacaattgat aagagtcctc atacataaag actccattag taagccagtg acccaggagc
     3661 ccagaccaac agcaaagcag acagtgacca tggtgagcaa gggcgaagag ctgttcaccg
     3721 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     3781 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     3841 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     3901 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     3961 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     4021 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     4081 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     4141 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     4201 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     4261 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     4321 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     4381 ctctcggcat ggacgagctg tacaagtaaa gcggcccctc tcgagcctct agaactatag
     4441 tgagtcgtat tacgtagatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     4501 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     4561 tgtaaccatt ataagctgca ataaacaaga gatctgatct agaggatcat aatcagccat
     4621 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     4681 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     4741 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     4801 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcatcat cgatgcgtct
     4861 tttgttctgg tcatcaagga ggggggaatg ttctgcgcat gcctgtgggg gagggagaag
     4921 gacacgtcac tgaaaacgtc cctgcatcac accgagacac ccaatcactc aagccgagac
     4981 cagataattt tgcatatgct ttaaagtttg aaaaatacca cggtaaaccc tcacacaaac
     5041 tctggatttg agatctttca ggttttatca gtttgcaggt ttatgtcacc atgatatagg
     5101 gtcagacttg atctaaggga gctgaataag tggtttagtc actcaccacc tcccaaaaac
     5161 atacccagaa gtccctggta tatatagctc tccctccagc tcttggttcg gctagcactc
     5221 ctcagtgacg aggagtcagt agcgttttag agctagaaat agcaagttaa aataaggcta
     5281 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttggtac cgtaaaacga
     5341 cggccagtga attatcaact atgtataata aagttgctgg catacactca cttccactaa
     5401 taaaatgaga atattttcaa tgcagaacat ctgtatggag cactttagat tcacacagtt
     5461 cacctgatca atcaaatata aatatgtcaa gatcaaaaac aatgtgcact caccaagtca
     5521 atgcacagac aatatcagtg tgtggtgtgt tatttcacag aaactaactg cacatctaaa
     5581 aataataaac ctaccgtgag aaggagagta caaagtgaac gtggcataca cagagtgagc
     5641 cgatccgagc agcaaagcca tgtagcaaag acaaagaaaa acatcaggca ggaaatttgg
     5701 gcaacatttt atttataggc tttatctttt cagaacatca aatctcaaca gtctccacag
     5761 gttttgacat gttcagttaa cggtggctga gacttaatag ccactttgta caagaaagct
     5821 gggtctctag attccgcggt cacacctttc tcttcttctt aggagatctc accggtgagt
     5881 cgccacccag ctgagacagg tcaatccttg tttcgtacag gccggtaata ctctgatgga
     5941 tcagtgtagc gtccagcacc tccttagtgg aggtgtacct ctttctatcg attgtagtgt
     6001 cgaagtactt gaaagctgca ggtgcgccca ggtttgtcag agtgaacaga tgaatgatat
     6061 tttcggcctg ctccctgatg ggcttatctc tgtgtttgtt gtaagcagac aggaccttat
     6121 ccagatttgc gtcggccagg atcactcttt tagagaactc gctaatctgt tcaatgatct
     6181 cgtccagata atgcttgtgc tgctccacaa acagctgttt ctgttcgtta tcctctggag
     6241 atcctttcag cttctcgtag tggcttgcca gatacaggaa gttcacgtac ttagaaggca
     6301 gggccagttc attgcctttc tgcagctctc cagcgcttgc cagcatgcgt ttcctgccgt
     6361 tttccagctc aaacagactg tacttgggca gtttaatgat caggtctttc ttcacttcct
     6421 tataaccttt ggcctccaga aaatcgattg ggttcttctc gaaagagctt ctctccataa
     6481 ttgtgatacc cagcagctcc ttgacagatt tcagtttctt gcttttgccc ttctccacct
     6541 tagcgaccac caggacggag tatgccacag tgggagagtc aaagcctcca tacttcttag
     6601 ggtcccagtc tttctttcta gcgatcagct tatcagagtt gcgttttggc agaatacttt
     6661 cttttgagaa accgcctgtc tgcacctcag ttttcttgac gatattcact tgaggcatgc
     6721 tcaggacctt cctcacggtt gcaaaatctc tgcctttgtc ccacacgatt tcgcctgtct
     6781 ctccgtttgt ctcaatcagg ggccttttgc ggatttctcc attggccagg gtgatctctg
     6841 tcttaaagaa attcatgatg tttgagtaaa agaaatactt ggcagtagct tttccaattt
     6901 cctgctcgga cttggcgatc atttttctga catcgtacac cttatagtct ccgtacacga
     6961 attcgctctc cagttttggg tatttcttga tcagtgcggt accgaccacg gcgttcaggt
     7021 atgcgtcatg ggcatggtga taattgttaa tctcgcggac cttataaaac tggaaatctt
     7081 tcctaaagtc gctgaccagt ttactcttca gggtaatgac cttcacttct ctgatcagtt
     7141 tgtcattctc atcgtacttt gtgttcatgc gactatccag aatctgtgcg acgtgtttag
     7201 tgatctggcg tgtttccacc agctgcctct tgatgaatcc agctttgtcc agttctgaca
     7261 gtccaccgcg ctctgctttt gtcaggttat caaacttcct ctgagtgatc agtttggcat
     7321 tcagcagctg tctccagtag ttcttcattt tcttgaccac ttcctcacta gggacattat
     7381 ctgattttcc tctgtttttg tcggacctgg tcagcacctt gttgtcaatg ctgtcatctt
     7441 tgatgaaact ctgggggacg atatgatcca cgtcgtaatc agacagcctg ttaatatcca
     7501 gctcctggtc cacgtacata tctcttccat tctgcagata gtacagatac agcttctcat
     7561 tctgcagctg tgtgttttcc actgggtgct ctttcaggat ctggctaccc agctccttaa
     7621 tgccttcctc gattcttttc atcctctctc tactgttctt ctgacccttc tgggttgtct
     7681 gattttcgcg agccatctca atcacgatgt tttcgggctt atggcccatg actttcacca
     7741 gctcatcgac gattttcact gtctgcagaa tgcctttctt gatggctggg cttccagcca
     7801 ggtttgcaat ctgctcatgc agggagtggc cctgtccaga cacctgagcc ttctggatgt
     7861 cctctttgaa ggtcagactg tcatcgtgaa tcagctgcat aaaattgcgg ttggcgaagc
     7921 catcactttt cagaaagtcc aggattgtct ttcctgactg tttatcccta atgccgttga
     7981 tcagcttcct gctcagtcta ccccagccag tgtagcgcct tctcttcagc tgtttcatca
     8041 ccttgtcatc gaacagatga gcataggttt tcagtctttc ctcgatcatt cctctgtcct
     8101 caaacagggt cagtgtcagc acaatgtctt ccaggatatc ctcattttcc tcgttgtcca
     8161 ggaagtcttt atccttaatg atcttcagca gatcgtggta agcgcccagg cttgcgttaa
     8221 atctgtcttc gactccgctg atctccacgg aatcgaagca ttcaattttc ttgaagtagt
     8281 cctccttcag ctgcttgaca gtcactttcc tgttggtttt aaacagcaga tccacgattg
     8341 ctttcttctg ctctcctgac aggaaggctg gctttctcat gccctcggtg acgtatttca
     8401 cctttgtcag ttcgttatag acagtgaaat actcgtacag cagggagtgc ttaggcagca
     8461 ctttctcatt gggcaggttt ttgtcaaaat tagtcattct ctcgatgaag gactgtgcag
     8521 aggcgccctt atcgaccact tcctcgaagt tccaaggagt aatggtttcc tcggatttgc
     8581 gagtcatcca ggcaaatctg ctgtttccgc gagccagtgg acccacatag taagggattc
     8641 tgaaggtcag aattttttcg atcttctcgc ggttatcttt cagaaagggg tagaagtctt
     8701 cctggcgcct caggatagcg tgcagctctc ccagatgaat ctggtgtggg atggaaccgt
     8761 tatcaaatgt gcgctgcttc ctcagcaggt cctctctatt cagtttcacc agcagttcct
     8821 cagttccgtc catcttctcc agaataggtt tgataaactt gtagaattcc tcctgtgatg
     8881 cgcctccgtc gatataaccg gcgtagccgt tcttggactg atcaaagaaa atttccttgt
     8941 acttctctgg cagctgctgc ctgaccagag ccttcagcag ggtcagatcc tgatggtgct
     9001 cgtcatatct cttgatcatg ctggcactca gaggtgcttt tgtaatctcg ctgttcactc
     9061 tcagaatatc tgacagcagg attgcgtcgg acagattttt ggcagccagg aacaggtcag
     9121 cgtactgatc tccgatctgt gccagcaggt tatccaggtc atcgtcatat gtgtcctttg
     9181 acagctgcag tttggcgtcc tcagccagat caaaattgct tttgaagtta ggagtcaggc
     9241 ccagactcag agcgatcaga tttccaaaca gaccgttcct cttttctccg ggcagctgtg
     9301 caatcaggtt ctccagtctg cgtgacttgg acagcctggc tgacaggata gcttttgcgt
     9361 ccactctgga agcattaata gggttttcct caaacagctg attgtaaatc tggaccagct
     9421 ggatgaacag cttgtccaca tcggaattgt cggggttcag atcgccctcg atcagaaaat
     9481 gacccctgaa cttaatcatg tgggccagag ccagatagat cagtctcagg tcggctttat
     9541 cagtagagtc agccagtttc ttgcgcagat gatagatggt tgggtatttt tcgtgatagg
     9601 cgacctcgtc cacaatgttt ccaaagatag gatggcgctc gtgtttctta tcttcctcga
     9661 ccaggaaact ttcctccagc ctatgaaaga atgaatcgtc caccttagcc atttcgttag
     9721 agaaaatctc ctgcaggtaa cagattctat tcttccttct ggtatagcgc cttcttgctg
     9781 ttcttttcag gcgtgttgct tcggcagtct ctccagaacc gaacagcagg gctccaatca
     9841 gatttttctt gatgctgtgc ctgtcggtgt tacccagcac cttaaatttc ttgctaggga
     9901 ccttgtagtc gtctgtaatc actgcccaac ccacggagtt agttccaata tccaggccga
     9961 tgctatactt cttatccatg cttcccacct ttctcttctt cttaggtgga gaagccatag
    10021 ggccgggatt ctcttccacg tcaccgcatg tcagcagact gcctctgccc tcaggcctag
    10081 gcttgtacag ctcgtccatg ccattaagtt tgtgccccag tttgctaggg aggtcgcagt
    10141 atctggccac agccacctcg tgctgctcga cgtaggtctc tttgtcggcc tccttgattc
    10201 tttccagtct gtggtccaca tagtagacgc cgggcatctt gaggttctta gcgggtttct
    10261 tggatctgta tgtggtcttg aagttgcaga tcaggtggcc cccgcccacg agcttcaggg
    10321 ccatgtcggt tctgccttcc aggccgccgt cagcggggta cagcatctcg gtgttggcct
    10381 cccagccgag tgttttcttc tgcatcacag ggccgttgga tgggaagttc acccctctga
    10441 tcttgacgtt gtagatgagg cagccgtcct ggaggctggt gtcctgggta gcggtcagca
    10501 cgcccccgtc ttcgtatgtg gtgactctct cccatgtgaa gccctcaggg aaggactgct
    10561 taaagaagtc ggggatgccc tgggtgtggt tgatgaaggt tctgctgccg tacatgaagc
    10621 tggtagccag gatgtcgaag gcgaagggga gagggccgcc ctcgaccacc ttgattctca
    10681 tggtctgggt gccctcgtag ggcttgcctt cgccctcgga tgtgcacttg aagtggtggt
    10741 tgttcacggt gccctccatg tacagcttca tgtgcatgtt ctccttaatc agctcttcgc
    10801 ccttagacac cgtcagtttt gttgcttctt tatccttaca ttgcacacag cccatggtgg
    10861 cagcctgctt ttttgtacaa acttggcctg ctgctggtgt catttactgg ctgaacgatg
    10921 atcagcaaac tcagatgtct tatttatctt gagctgaatc tgaaccggac tgttcagcac
    10981 tgaatctata ggccgattgt gtgtgtgtgt gtgtgtgtgt agcaacaatt ctgataaaag
    11041 cttgactctc gcggcctaat tgatttattt acagtgatcc ggtcttagtg ggaagcccct
    11101 ttcatgttga ttgatcaccg caacatgcag cagatttaac agcacaacaa acctttttgt
    11161 actaatgacc caggagcatt aaagctctcg ggcaacgtct ccattcacgc aaaagtgtgg
    11221 atttgcagct cacgtgagag aggtgacttg gtttatgtgt tcctatgaag ctgcccataa
    11281 tgaacaattt ctcctaatgg caaattatcc ctgtttctct ttcctgaagc cgaggcccct
    11341 gcaaattgat gtatctgtcc tccgagggtt tcaccgtcac attacggcgt tttaaaatat
    11401 taatatgaga gtgcatttag ccaggagtag cgaggcacgt tgctttcatg taggtcacaa
    11461 gagactgact ccctctccct ctgtctgttt ctgtctgtct ctttcatttt atctctctct
    11521 ctctttcttt tctcctttct cgctctcttt ttcatctggc tagttcagat attctgtttt
    11581 tttttgtaaa taactgaatg aagagttgat atatatattt gtgtatgtgt gtgtgtgtgt
    11641 atgtttgcta ttatttagat tttctgactt tctttgcttg gcattcagtg tgtagaggtg
    11701 ccctctcaaa cactgattaa agcagacgac gtgtcacatc ctgttaccaa cttttctata
    11761 caaagttgat agcttggcgt aatcatggtc atagctgttt cctgctcgag ggcccatctg
    11821 gcctgtgttt cagacaccag ggagtctctg ctcacgtttc ctgctatttg cagcctctct
    11881 atcaagacta atacacctct tcccgcatcg gctgcctgtg agaggctttt cagcactgca
    11941 ggattgcttt tcagccccaa aagagctagg cttgacacta acaattttga gaatcagctt
    12001 ctactgaagt taaatctgag gttttacaac tttgagtagc gtgtactggc attagattgt
    12061 ctgtcttata gtttgataat taaatacaaa cagttctaaa gcaggataaa accttgtatg
    12121 catttcattt aatgtttttt gagattaaaa gcttaaacaa gaatctctag ttttctttct
    12181 tgcttttact tttacttcct taatactcaa gtacaatttt aatggagtac ttttttactt
    12241 ttactcaagt aagattctag ccagatactt ttacttttaa ttgagtaaaa ttttccctaa
    12301 gtacttgtac tttcacttga gtaaaatttt tgagtacttt ttacacctct gtcaagaact
    12361 cctggacaaa cctctgacct gtgtggaaca gagtggatat gggtgtctga acagatattc
    12421 acgtcttttg cagatcagag ggcatttctg gtgttgtgca atgacctggg tccaacaggt
    12481 aggcgtactc gtatccaaga gcattgttgg acaggaggta gtctccattc ctataaagag
    12541 gaaggaatgg caccatgtag tagccgtcat tgtggccaat tggtgcattg gtacgtgggt
    12601 agatggaccg gggaggctga tgagttctta gccagccttt ccccgtcaag ctctaaatcg
    12661 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
    12721 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
    12781 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
    12841 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
    12901 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
    12961 ttaggtggca c
//
