LOCUS       sequence-391634-        1623 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For SnoopLigase2 expression within secretory pathway of mammalian 
            cells. N775Q mutation to remove glycosylation site, KDEL to retain 
            in Endoplasmic Reticulum, MBP for improved solubility.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Keeble AH, Wood DP, Howarth M
  TITLE     Design and Evolution of Enhanced Peptide-Peptide Ligation for 
            Modular Transglutaminase Assembly.
  JOURNAL   Bioconjug Chem. 2023 Jun 8. doi: 10.1021/acs.bioconjchem.3c00122.
  PUBMED    37289810
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bioconjug 
            Chem. 2023 Jun 8. doi: 10.1021/acs.bioconjchem.3c00122."
COMMENT     Sequence Label: pcDNA3.1 HA-MBP-SnoopLigase2 N775Q-KDEL
FEATURES             Location/Qualifiers
     source          1..1623
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             73..99
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             115..1212
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="KIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEE
                     KFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLI
                     AYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAA
                     DGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGET
                     AMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLE
                     NYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFW
                     YAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     1186..1209
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1243..1260
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgaacaccc agatcctggt gttcgccctg atcgccatca ttcccaccaa cgccgacaag
       61 atcggatctg gctacccata cgatgttcca gattacgcta gtggagaaag tggtaaaatc
      121 gaagaaggta aactggtaat ctggattaac ggcgataaag gctataacgg tctcgctgaa
      181 gtcggtaaga aattcgagaa agataccgga attaaagtca ccgttgagca tccggataaa
      241 ctggaagaga aattcccaca ggttgcggca actggcgatg gccctgacat tatcttctgg
      301 gcacacgacc gctttggtgg ctacgctcaa tctggcctgt tggctgaaat caccccggac
      361 aaagcgttcc aggacaagct gtatccgttt acctgggatg ccgtacgtta caacggcaag
      421 ctgattgctt acccgatcgc tgttgaagcg ttatcgctga tttataacaa agatctgctg
      481 ccgaacccgc caaaaacctg ggaagagatc ccggcgctgg ataaagaact gaaagcgaaa
      541 ggtaagagcg cgctgatgtt caacctgcaa gaaccgtact tcacctggcc gctgattgct
      601 gctgacgggg gttatgcgtt caagtatgaa aacggcaagt acgacattaa agacgtgggc
      661 gtggataacg ctggcgcgaa agcgggtctg accttcctgg ttgacctgat taaaaacaaa
      721 cacatgaatg cagacaccga ttactccatc gcagaagctg cctttaataa aggcgaaaca
      781 gcgatgacca tcaacggccc gtgggcatgg tccaacatcg acaccagcaa agtgaattat
      841 ggtgtaacgg tactgccgac cttcaagggt caaccatcca aaccgttcgt tggcgtgctg
      901 agcgcaggta ttaacgccgc cagtccgaac aaagagctgg caaaagagtt cctcgaaaac
      961 tatctgctga ctgatgaagg tctggaagcg gttaataaag acaaaccgct gggtgccgta
     1021 gcgctgaagt cttacgagga agagttggcg aaagatccac gtattgccgc cactatggaa
     1081 aacgcccaga aaggtgaaat catgccgaac atcccgcaga tgtccgcttt ctggtatgcc
     1141 gtgcgtactg cggtgatcaa cgccgccagc ggtcgtcaga ctgtcgatga agccctgaaa
     1201 gacgcgcaga ctaattcgag ctcgggtagc agtggtgcag cacatcatca tcaccatcat
     1261 agctctggtg gtagtggtgt gaataagaac gataaaaagc cgctgcgtgg tgccgtgttt
     1321 agcctgcaga aacagcatcc cgactatccc gatatctatg gcgcgattga tcagcagggg
     1381 acctatcaaa atgtgcgtac cggcgaagat ggtaaactga cctttaagaa tctgagcgat
     1441 ggcaaatatc gcctgattga aaatagcgaa cccccgggct ataaaccggt gcagaataag
     1501 ccgattgtga gctttcgtat tgtggatggc gaagtgcgtg atgtgaccag cattgtgccg
     1561 ccgggtgtgc cggctacata tgaatttacc ggaggaggtg gctctaaaga tgaactgtag
     1621 tag
//
