LOCUS       sequence-391663-        4703 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Co-expression plasmid for the CEP neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pWP095
FEATURES             Location/Qualifiers
     source          1..4703
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2448..2552
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2553..2621,2622..3413)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2771..2790)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3584..4172
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4073..4092
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4326..4343
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4460..4481
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4496..4501,4502..4519,4520..4526)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4534..4550
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4539..4561
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4558..4574
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4558..4574
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggccacac aatttacgat gtcgttgttc
       61 gtttttttct aaacttcgaa attccaatca ttttaataaa tgtttatcaa aaaacgtttt
      121 ttttttaatt ttccagggtt ttgagttcca tcaaccaact aatgtcgata gaagcaaatt
      181 ttgcatagtt tcataaaatt ctggaggtac tgtagaagtg taggattact gtagttttcg
      241 aaaaaaaagg ttttgtcgtt ggaaagatat ggatctgagg ttagtgagga gatatgggcg
      301 ggttacggtg attgtatcgt aggattactg tagtttttgg aaaaaagagc atggtcgggg
      361 tagattagtg gtaatgtaga ggtactgtag gattactgta attattgaaa aaagtaaact
      421 tttgactttt gaagggatat taagttgtcg ttgatgatgt tgacgcagta ctgcactagt
      481 actgtaggcg tatttaggag ttctgtaaga ttactgtaat taaaaaagtt aacttttgta
      541 gtagtactgt aaaagggact gtagtacgag aattgactta aacagtgtga tatttttatt
      601 ttttcgataa acttaaccaa aaatgtagac catcagtcag tatcacttgt tcttagcatg
      661 gttcaaaaaa taattaggac aactctacca gtgctcctga ttttctaggc cagctaaatg
      721 tcattcccaa aatacaaaca tgaaaatctg catttcttgt caaacttccc tactctcccg
      781 cttcctcgcg cggtttttga gtccttccgg acatttttcg acgaaacaca catgtagtag
      841 tgtctaaacg attatgaatt gtgtattgtg tcggctctca gagcccattt gaccccgccg
      901 ttttcgccca ttatttcaca tttcgagaag ctgcccccgc tgcacaacta gaaacatatg
      961 tgtgtagctc tgttttctac gatttcaaca tttccgttcg ttctttttaa tctttgaaat
     1021 aaacacatac acaaaggcgc gcctctagag gatccccggg attggccaaa ggacccaaag
     1081 gtatgtttcg aatgatacta acataacata gaacattttc aggaggaccc ttggctagga
     1141 tgggccctaa aaagaagcgt aaagctagca tgaagctgtt gtcttcaatg gaacaagctt
     1201 gcgacatatg ccggttaaaa aaactcaggt gctcgaagga aaagcccaag tgtgccaagt
     1261 gtctgaagaa caactcggag tgccgttatt ctcccaaggc tatgaggtcc ccactgacca
     1321 gggcgcatct gacagaggta gaatcaaggt tggaaaaatt ggaagatttg tttctgctca
     1381 tgtttcctcg ggaaaacctg gacagcattt tgaacatgga ctctttggac gaagtgaaag
     1441 taatgttgaa acaattatat atgcaggact atggtgacac ggatgctgct atggatagct
     1501 tgacccctat ggaaaatggc atgtctgaag ggccgagaaa acacatgaag actgcgacat
     1561 catcgccaaa agtggatgaa gatggaagcc aaagtcagtt gagtgtatcg ccggaattcc
     1621 cgctgcagtc tggtggcgga ggggctccta ggtgcctcga cctcaagacc caagtccaaa
     1681 ccccacaagg aatgaaggag atctccaaca tccaagtcgg agacctcgtc ctctccaaca
     1741 ccggatacaa cgaggtcctc aacgtcttcc caaagtccaa gaagaagtcc tacaagatca
     1801 ccctcgagga cggaaaggag atcatctgct ccgaggagca cctcttccca acccaaaccg
     1861 gagagatgaa catctccgga ggactcaagg agggaatgtg cctctacgtc aaggagtaag
     1921 gtaccgaatt cgctagccgg ccatacaagt aatccggatg atcgacgcca acgtcgttga
     1981 attttcaaat tttaaatact gaatatttgt tttttttcct attatttatt tattctcttt
     2041 gtgttttttt tcttgctttc taaaaaatta attcaatcca aatctaaaca tttttttttc
     2101 tctttccgtc tcccaattcg tattccgctc ctctcatctg aacacaatgt gcaagtttat
     2161 ttatcttctc gctttcattt cattaggacg tggggggaat tggtggaagg gggaaacaca
     2221 caaaaggatg atggaaatga aataaggaca cacaatatgc aacaacattc aattcagaaa
     2281 tatggaggaa ggtttaaaag aaaacataaa aatatataga ggaggaagga aaactagtaa
     2341 aaaataagca aagaaattag gcgaacgatg agaattgtcc tcgcttgggc ccaaaaggcc
     2401 tttttttttt ggcgccgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     2461 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     2521 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     2581 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     2641 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     2701 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     2761 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     2821 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     2881 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     2941 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     3001 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     3061 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     3121 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     3181 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     3241 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     3301 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     3361 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     3421 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     3481 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     3541 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3601 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3661 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3721 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3781 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3841 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3901 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3961 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     4021 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4081 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     4141 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     4201 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     4261 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     4321 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     4381 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     4441 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     4501 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     4561 gaaacagcta tgaccatgat tacgccaagc tgtaagttta aacatgatct tactaactaa
     4621 ctattctcat ttaaattttc agagcttaaa aatggctgaa atcactcaca acgatggata
     4681 cgctaacaac ttggaaatga aat
//
