LOCUS       sequence-391665-        4460 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drives specific expression in the AFD neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pWP004
FEATURES             Location/Qualifiers
     source          1..4460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1518..1667
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        2205..2309
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2310..2378,2379..3170)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2528..2547)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3341..3929
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3830..3849
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4083..4100
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4217..4238
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4253..4258,4259..4276,4277..4283)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4291..4307
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4296..4318
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4315..4331
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4315..4331
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggccattg tattgcaatc agtgtaatct
       61 ttttctattg ttgttctgat cagttgaatt aatttgggtt tcaaaataac tgaaagggaa
      121 gccaattttt aacggggaag ctctgcataa tttctcaaaa caacttatta gttagcttat
      181 actaattgaa aaaaaaagaa gatagctaat ttttggcaag cttaaactta agtttctaaa
      241 tgtttccaat atctgcctat tcgaactacc gcgcttttgc ctcaaactcc gcctaatttt
      301 ccgtgccgca tcgtggtttt tatcaatttc gacgcaaact ctttgttttg aatcgttaaa
      361 attttttgta cattagacgt taagggaata ttgtaataga cacttcactt gaaaatttaa
      421 aattggagaa aaatcgaggt gtagcaaaat gggcggagtt ttgagacaaa accacggtag
      481 ttcaaatgag gagattaaat agttacaatt aaaaattcgc aaaaaaaact tgcattgtct
      541 gaatgatgtt ttttattatt ttgttgtgtc tcggcttcta ggtccagtct gtgtatttta
      601 tttcattgta ccataaacgt gtgaatagag ttctcgtaaa tcgacaatgg agttttcaaa
      661 aaatacataa cgatgagtaa tccttgttat attttccatt agagtaaatt gatataaaga
      721 aaacagagtt atatctgaag ttgagaagct taaaagattt atgactaccg taagaatatc
      781 cggatgagct gatacagatg gctcatgaag agacgcaacg ggtttcattt cgtacatttt
      841 tatcaactca tattcgatcc acggtaacta tcgtgagaag ctgtaggcgc gcctctagag
      901 gatccccggg attggccaaa ggacccaaag gtatgtttcg aatgatacta acataacata
      961 gaacattttc aggaggaccc ttggctagga tgggccctaa aaagaagcgt aaagctagca
     1021 tgaagctgtt gtcttcaatg gaacaagctt gcgacatatg ccggttaaaa aaactcaggt
     1081 gctcgaagga aaagcccaag tgtgccaagt gtctgaagaa caactcggag tgccgttatt
     1141 ctcccaaggc tatgaggtcc ccactgacca gggcgcatct gacagaggta gaatcaaggt
     1201 tggaaaaatt ggaagatttg tttctgctca tgtttcctcg ggaaaacctg gacagcattt
     1261 tgaacatgga ctctttggac gaagtgaaag taatgttgaa acaattatat atgcaggact
     1321 atggtgacac ggatgctgct atggatagct tgacccctat ggaaaatggc atgtctgaag
     1381 ggccgagaaa acacatgaag actgcgacat catcgccaaa agtggatgaa gatggaagcc
     1441 aaagtcagtt gagtgtatcg ccggaattcc cgctgcagtc tggtggcgga ggggctccta
     1501 ggggttccgg acgggctgac gcattggacg attttgatct ggatatgctg ggaagtgacg
     1561 ccctcgatga ttttgacctt gacatgcttg gttcggatgc ccttgatgac tttgacctcg
     1621 acatgctcgg cagtgacgcc cttgatgatt tcgacctgga catgctgatt aactaaggta
     1681 ccgaattcgc tagccggcca tacaagtaat ccggatgatc gacgccaacg tcgttgaatt
     1741 ttcaaatttt aaatactgaa tatttgtttt ttttcctatt atttatttat tctctttgtg
     1801 ttttttttct tgctttctaa aaaattaatt caatccaaat ctaaacattt ttttttctct
     1861 ttccgtctcc caattcgtat tccgctcctc tcatctgaac acaatgtgca agtttattta
     1921 tcttctcgct ttcatttcat taggacgtgg ggggaattgg tggaaggggg aaacacacaa
     1981 aaggatgatg gaaatgaaat aaggacacac aatatgcaac aacattcaat tcagaaatat
     2041 ggaggaaggt ttaaaagaaa acataaaaat atatagagga ggaaggaaaa ctagtaaaaa
     2101 ataagcaaag aaattaggcg aacgatgaga attgtcctcg cttgggccca aaaggccttt
     2161 tttttttggc gccgacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     2221 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     2281 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     2341 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     2401 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     2461 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     2521 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     2581 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     2641 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     2701 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     2761 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     2821 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     2881 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     2941 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     3001 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     3061 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     3121 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     3181 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     3241 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3301 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3361 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3421 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3481 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3541 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3601 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3661 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3721 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3781 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3841 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3901 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3961 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     4021 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     4081 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     4141 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     4201 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     4261 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     4321 acagctatga ccatgattac gccaagctgt aagtttaaac atgatcttac taactaacta
     4381 ttctcattta aattttcaga gcttaaaaat ggctgaaatc actcacaacg atggatacgc
     4441 taacaacttg gaaatgaaat
//
