LOCUS       sequence-391674-        4626 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drives specific expression in the ADL neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pWP169
FEATURES             Location/Qualifiers
     source          1..4626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1684..1833
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        2371..2475
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2476..2544,2545..3336)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2694..2713)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3507..4095
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3996..4015
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4249..4266
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4383..4404
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4419..4424,4425..4442,4443..4449)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4457..4473
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4462..4484
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4481..4497
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4481..4497
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggccattc tcccgcacgg agtcacacga
       61 gtgttttatt ttttagccca aggcaacaaa aattgcttaa actggaaaat atcaggatat
      121 tccgccatat cgatttctgt ggatatatag aaaatgataa tattcggaac tagtctatgt
      181 tgtccgaaga aaaaaatctt acggcctgag aagtgatacc tgtaagcaat ttgtctacaa
      241 aaaagcctgc aaacaccgac tacagtaccc caaaacaccc gattttaata tattggtcag
      301 ttttcagctg aaaattgatt tttcgaagtt aattgagtgc tgttttcaaa aatcgactgc
      361 aaattgaggt ggaaatgcag gaaatttttt ttttgagagt ttcagcattt tcaatcgaaa
      421 aatgtgaatt ttcatgcgaa aatcatgttt cgcattccca attatccatt tttcccgttt
      481 tcccacaatt ctagcttgaa aataaacatg acagttttgg gattacagta gtcttcaacg
      541 gcacacacct tttcgcatca atcaaaaact tgtcttgtcg tgtcgagacc gggaaccgta
      601 tttttggcag aaaaatcgca aaattttgcg tctgggtagg cattttcgcc cctgcgatgt
      661 gtcggaatat aattttgcag tcacttcttt gttttccatt tttttagctg aatcatccac
      721 tggtaacatt atgtaatgac tatatttata aaaagccgtt ccaaaccaca tacagaaaat
      781 aagcaacaac aagaaaaagc aaagtgaata ttaaaaatag aggataatgt cacactgtca
      841 cactaattag tcattgctga aaacatttaa acctatactt aaaatctttt ctcgtcttca
      901 ctctcctcta tctcgcttat tcaaagaatc taaaaacact agagattgtg ggttgtttct
      961 gaaaaaaaaa cgaattcacc tgtcacagca aacttaaagt tttcttcgaa ttgtgagctc
     1021 atttctcttt tgattttatt ttcattccaa cggcgcgcct ctagaggatc cccgggattg
     1081 gccaaaggac ccaaaggtat gtttcgaatg atactaacat aacatagaac attttcagga
     1141 ggacccttgg ctaggatggg ccctaaaaag aagcgtaaag ctagcatgaa gctgttgtct
     1201 tcaatggaac aagcttgcga catatgccgg ttaaaaaaac tcaggtgctc gaaggaaaag
     1261 cccaagtgtg ccaagtgtct gaagaacaac tcggagtgcc gttattctcc caaggctatg
     1321 aggtccccac tgaccagggc gcatctgaca gaggtagaat caaggttgga aaaattggaa
     1381 gatttgtttc tgctcatgtt tcctcgggaa aacctggaca gcattttgaa catggactct
     1441 ttggacgaag tgaaagtaat gttgaaacaa ttatatatgc aggactatgg tgacacggat
     1501 gctgctatgg atagcttgac ccctatggaa aatggcatgt ctgaagggcc gagaaaacac
     1561 atgaagactg cgacatcatc gccaaaagtg gatgaagatg gaagccaaag tcagttgagt
     1621 gtatcgccgg aattcccgct gcagtctggt ggcggagggg ctcctagggg ttccggacgg
     1681 gctgacgcat tggacgattt tgatctggat atgctgggaa gtgacgccct cgatgatttt
     1741 gaccttgaca tgcttggttc ggatgccctt gatgactttg acctcgacat gctcggcagt
     1801 gacgcccttg atgatttcga cctggacatg ctgattaact aaggtaccga attcgctagc
     1861 cggccataca agtaatccgg atgatcgacg ccaacgtcgt tgaattttca aattttaaat
     1921 actgaatatt tgtttttttt cctattattt atttattctc tttgtgtttt ttttcttgct
     1981 ttctaaaaaa ttaattcaat ccaaatctaa acattttttt ttctctttcc gtctcccaat
     2041 tcgtattccg ctcctctcat ctgaacacaa tgtgcaagtt tatttatctt ctcgctttca
     2101 tttcattagg acgtgggggg aattggtgga agggggaaac acacaaaagg atgatggaaa
     2161 tgaaataagg acacacaata tgcaacaaca ttcaattcag aaatatggag gaaggtttaa
     2221 aagaaaacat aaaaatatat agaggaggaa ggaaaactag taaaaaataa gcaaagaaat
     2281 taggcgaacg atgagaattg tcctcgcttg ggcccaaaag gccttttttt tttggcgccg
     2341 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     2401 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     2461 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     2521 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     2581 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     2641 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     2701 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     2761 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     2821 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     2881 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     2941 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     3001 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     3061 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     3121 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     3181 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     3241 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     3301 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     3361 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     3421 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     3481 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     3541 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     3601 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     3661 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     3721 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     3781 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     3841 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     3901 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     3961 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4021 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4081 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     4141 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     4201 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     4261 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     4321 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     4381 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     4441 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     4501 gattacgcca agctgtaagt ttaaacatga tcttactaac taactattct catttaaatt
     4561 ttcagagctt aaaaatggct gaaatcactc acaacgatgg atacgctaac aacttggaaa
     4621 tgaaat
//
