LOCUS       sequence-391678-        5511 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drives specific expression in the ADF neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 5511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pCC12
FEATURES             Location/Qualifiers
     source          1..5511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             479..490
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2569..2718
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        3256..3360
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3361..3429,3430..4221)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3579..3598)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4392..4980
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4881..4900
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5134..5151
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5268..5289
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5304..5309,5310..5327,5328..5334)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5342..5358
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5347..5369
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5366..5382
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5366..5382
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggccaatg tcgctattca actgtgtcaa
       61 gccatcaatg catttgctta ttgttcactg atatctatta aaattattga taacttcagc
      121 aacacttgca ggatcacttt gttggtactg gtcaaaagca gcaacgaaat catacaactg
      181 gtccaactgc gcgcattttg acttgaacag cttatatttt tgaatagact aaactttttt
      241 gttgagatac tggaaatttc cgacaagtta gacgatttcc gagagaattc caatataaag
      301 ttcaatgtgc ccatcataac cgtcaaatcg tcattcaaca aggtttgtgt atcattttta
      361 gataattgta tcaacttcgt gtactcttga tgcatccttt ttaactccga acttgccgcc
      421 ttaacattta cgttgagaac cgtattcaac tgatcgctgg gcatttgaag aagttctgat
      481 agaaggtcgc gagctttgat ttccttccga attgctccag cttgaaggga aattgcattg
      541 gtgacacgcg atagaatccg aaactgttca ctgattgctt ggagatcgga tgaagctgaa
      601 catttttatg tttgcagcgg agcattgttg tttacagaga gtgtagatag ttgaagacac
      661 acagacatcc cggaacccaa gaggttgggc gcgggaagag ccgattagtg ccgatttacg
      721 gagttgttct tttcacaatt gccccgtaaa tctacacaaa tcggacaccc gccccttgag
      781 tcccgggatg cctgtgtctc ttcaactata tacactctct gtaccggtaa cacactatga
      841 catttacaca ctctctgacg gttttaaagc cctaaaaaat gaaatattcg atcttatcta
      901 gttttaggca caatttctgc cgggtgacta tcgattcaaa attcactgca atctagggct
      961 ccaatgaagt cgcgcctgcg cgccggcgct caaaacaaag gaaatttatc aaataatttg
     1021 atagtcaaac aaaatataag cttttcagca gcgacaatga caaattgata acatttcaga
     1081 gtttagcgac ggcttcggcc catctttctt ggtgcagaat ctcctaacag ttcacgcagg
     1141 cgcagttttg ctcataagac gccagcgctg gcgccgagtt tgttaaattg aataccacca
     1201 cttcatagac tcccctgatt tgccaatttt caaaaatcct aattgaataa cgataacgaa
     1261 ataaaaatta gttatttgca ttgttgttaa caccaatttt gaacttttct ctgaaatgtt
     1321 tcgtttaaaa ctgaacatct gaaatgtaaa ctcactgaaa gattatcact ttttttgaaa
     1381 agcagtagat tgggaagttg tccacatcgg caatgacagg gatggaagat ttcaatgacg
     1441 agataagacg atcaaatgaa aagtcacgga aataaggaaa acgtgcaaat ttattttatt
     1501 ttttgtaaaa gttttttcct ggagaaaaca cgtttggata tcaatattaa tttcgacacg
     1561 tcaaagcttt tttaaataat aatttcacta tcagaaaaaa cctaattcct catctttcat
     1621 aacattaatc ataatttcac tggtatatct aacatttttt gttttcaggg tgttatatac
     1681 attataagat cagtagtata ttaactttaa accactgaat ttcttctagt tttttgtttc
     1741 cagctcaagt gctcctgtac gcaaaacatt aggcttcttt gttcaaaaaa acattttctc
     1801 accacctcat gacatttttt ttctcatttt tcttattacg aatgtagcct tattagcata
     1861 tagcatagag gtacccaaaa ctagtttttt tttgttttga aatatatatt tgcacctctt
     1921 ttttcttttt gccaatggcg cgcctctaga ggatccccgg gattggccaa aggacccaaa
     1981 ggtatgtttc gaatgatact aacataacat agaacatttt caggaggacc cttggctagg
     2041 atgggcccta aaaagaagcg taaagctagc atgaagctgt tgtcttcaat ggaacaagct
     2101 tgcgacatat gccggttaaa aaaactcagg tgctcgaagg aaaagcccaa gtgtgccaag
     2161 tgtctgaaga acaactcgga gtgccgttat tctcccaagg ctatgaggtc cccactgacc
     2221 agggcgcatc tgacagaggt agaatcaagg ttggaaaaat tggaagattt gtttctgctc
     2281 atgtttcctc gggaaaacct ggacagcatt ttgaacatgg actctttgga cgaagtgaaa
     2341 gtaatgttga aacaattata tatgcaggac tatggtgaca cggatgctgc tatggatagc
     2401 ttgaccccta tggaaaatgg catgtctgaa gggccgagaa aacacatgaa gactgcgaca
     2461 tcatcgccaa aagtggatga agatggaagc caaagtcagt tgagtgtatc gccggaattc
     2521 ccgctgcagt ctggtggcgg aggggctcct aggggttccg gacgggctga cgcattggac
     2581 gattttgatc tggatatgct gggaagtgac gccctcgatg attttgacct tgacatgctt
     2641 ggttcggatg cccttgatga ctttgacctc gacatgctcg gcagtgacgc ccttgatgat
     2701 ttcgacctgg acatgctgat taactaaggt accgaattcg ctagccggcc atacaagtaa
     2761 tccggatgat cgacgccaac gtcgttgaat tttcaaattt taaatactga atatttgttt
     2821 tttttcctat tatttattta ttctctttgt gttttttttc ttgctttcta aaaaattaat
     2881 tcaatccaaa tctaaacatt tttttttctc tttccgtctc ccaattcgta ttccgctcct
     2941 ctcatctgaa cacaatgtgc aagtttattt atcttctcgc tttcatttca ttaggacgtg
     3001 gggggaattg gtggaagggg gaaacacaca aaaggatgat ggaaatgaaa taaggacaca
     3061 caatatgcaa caacattcaa ttcagaaata tggaggaagg tttaaaagaa aacataaaaa
     3121 tatatagagg aggaaggaaa actagtaaaa aataagcaaa gaaattaggc gaacgatgag
     3181 aattgtcctc gcttgggccc aaaaggcctt ttttttttgg cgccgacgtc aggtggcact
     3241 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3301 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     3361 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     3421 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     3481 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     3541 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     3601 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     3661 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     3721 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     3781 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     3841 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     3901 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     3961 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     4021 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     4081 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     4141 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     4201 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     4261 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     4321 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     4381 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     4441 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     4501 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     4561 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     4621 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     4681 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     4741 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     4801 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     4861 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     4921 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     4981 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     5041 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     5101 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     5161 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     5221 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     5281 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     5341 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctg
     5401 taagtttaaa catgatctta ctaactaact attctcattt aaattttcag agcttaaaaa
     5461 tggctgaaat cactcacaac gatggatacg ctaacaactt ggaaatgaaa t
//
