LOCUS       sequence-39168-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pTAL3
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(185..289)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    331..834
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 cttcggggcg aaaactctct aagattttgc cgctgttgag atccagttca atgtaaccca
       61 ctcgggcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
      121 aaacaggaag gcagaaggcc gcaaaaaagg gaataagggc gacacggaaa tgttggatac
      181 tcatactctt cctttttcaa tataattgaa gcatttatca gggttattgt ctcattagcg
      241 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttcatcgc acatttcccc
      301 gaaaagtgcc acctgaccct aaggcttttc atcacgtgct ataaaaataa ttataattta
      361 aattttttaa tataaatata taaattaaaa atagaaagta aaaaaagaaa ttaaagaaaa
      421 aatagttttt gttttccgaa gatgtaaaag actctagggg gatcgccaac aaatactacc
      481 ttttatcttg ctcttcctgc tctcaggtat taatgccgaa ttgtttcatc ttgtctgtgt
      541 agaagaccac acacgaaaat cctgtgattt tacattttac ttatcgttaa tcgaatgtat
      601 atctatttaa tctgcttttc ttgtctaata aatatatatg taaagtacgc tttttgttga
      661 aattttttaa acctttgttt attttttttt cttcattccg taactcttct accttcttta
      721 tttactttct aaaatccaaa tacaaaacat aaaaataaat aaacacagag taaattccca
      781 aattattcca tcattaaaag atacgaggcg cgtgtaagtt acaggcaagc gatccgtctt
      841 aattaa
//
