LOCUS       sequence-391702-        8739 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human TOLL protein expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8739)
  TITLE     TREAT-AD Plasmids 2023
REFERENCE   2  (bases 1 to 8739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCDNA3-hTLR4-VenusFlag
FEATURES             Location/Qualifiers
     source          1..8739
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    911..935
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3462..3486)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3497..3520
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(3551..3553,3554..3556,3557..4270)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3596..3617)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3857..3876)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4204..4225
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4310..4329)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4354..4402
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4604..5032
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4691..4710)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4901..4922
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5041..5061)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5046..5375
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5226..5361
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5288..5307
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5442..6236
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5496..5515)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6106..6125
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6412..6533
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6449..6468)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6503..6522
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6582..6598)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6582..6598)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6595..6617)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6606..6622
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6630..6636,6637..6654,6655..6660))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6675..6696
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6813..6830)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6984..7572)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7064..7083)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7743..8534,8535..8603))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8366..8385
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8604..8708)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgctagt
      901 taagctagca acaagtttgt acaaaaaagc aggctccacc atgatgtctg cctcgcgcct
      961 ggctgggact ctgatcccag ccatggcctt cctctcctgc gtgagaccag aaagctggga
     1021 gccctgcgtg gaggtggttc ctaatattac ttatcaatgc atggagctga atttctacaa
     1081 aatccccgac aacctcccct tctcaaccaa gaacctggac ctgagcttta atcccctgag
     1141 gcatttaggc agctatagct tcttcagttt cccagaactg caggtgctgg atttatccag
     1201 gtgtgaaatc cagacaattg aagatggggc atatcagagc ctaagccacc tctctacctt
     1261 aatattgaca ggaaacccca tccagagttt agccctggga gccttttctg gactatcaag
     1321 tttacagaag ctggtggctg tggagacaaa tctagcatct ctagagaact tccccattgg
     1381 acatctcaaa actttgaaag aacttaatgt ggctcacaat cttatccaat ctttcaaatt
     1441 acctgagtat ttttctaatc tgaccaatct agagcacttg gacctttcca gcaacaagat
     1501 tcaaagtatt tattgcacag acttgcgggt tctacatcaa atgcccctac tcaatctctc
     1561 tttagacctg tccctgaacc ctatgaactt tatccaacca ggtgcattta aagaaattag
     1621 gcttcataag ctgactttaa gaaataattt tgatagttta aatgtaatga aaacttgtat
     1681 tcaaggtctg gctggtttag aagtccatcg tttggttctg ggagaattta gaaatgaagg
     1741 aaacttggaa aagtttgaca aatctgctct agagggcctg tgcaatttga ccattgaaga
     1801 attccgatta gcatacttag actactacct cgatgatatt attgacttat ttaattgttt
     1861 gacaaatgtt tcttcatttt ccctggtgag tgtgactatt gaaagggtaa aagacttttc
     1921 ttataatttc ggatggcaac atttagaatt agttaactgt aaatttggac agtttcccac
     1981 attgaaactc aaatctctca aaaggcttac tttcacttcc aacaaaggtg ggaatgcttt
     2041 ttcagaagtt gatctaccaa gccttgagtt tctagatctc agtagaaatg gcttgagttt
     2101 caaaggttgc tgttctcaaa gtgattttgg gacaaccagc ctaaagtatt tagatctgag
     2161 cttcaatggt gttattacca tgagttcaaa cttcttgggc ttagaacaac tagaacatct
     2221 ggatttccag cattccaatt tgaaacaaat gagtgagttt tcagtattcc tatcactcag
     2281 aaacctcatt taccttgaca tttctcatac tcacaccaga gttgctttca atggcatctt
     2341 caatggcttg tccagtctcg aagtcttgaa aatggctggc aattctttcc aggaaaactt
     2401 ccttccagat atcttcacag agctgagaaa cttgaccttc ctggacctct ctcagtgtca
     2461 actggagcag ttgtctccaa cagcatttaa ctcactctcc agtcttcagg tactaaatat
     2521 gagccacaac aacttctttt cattggatac gtttccttat aagtgtctga actccctcca
     2581 ggttcttgat tacagtctca atcacataat gacttccaaa aaacaggaac tacagcattt
     2641 tccaagtagt ctagctttct taaatcttac tcagaatgac tttgcttgta cttgtgaaca
     2701 ccagagtttc ctgcaatgga tcaaggacca gaggcagctc ttggtggaag ttgaacgaat
     2761 ggaatgtgca acaccttcag ataagcaggg catgcctgtg ctgagtttga atatcacctg
     2821 tcagatgaat aagaccatca ttggtgtgtc ggtcctcagt gtgcttgtag tatctgttgt
     2881 agcagttctg gtctataagt tctattttca cctgatgctt cttgctggct gcataaagta
     2941 tggtagaggt gaaaacatct atgatgcctt tgttatctac tcaagccagg atgaggactg
     3001 ggtaaggaat gagctagtaa agaatttaga agaaggggtg cctccatttc agctctgcct
     3061 tcactacaga gactttattc ccggtgtggc cattgctgcc aacatcatcc atgaaggttt
     3121 ccataaaagc cgaaaggtga ttgttgtggt gtcccagcac ttcatccaga gccgctggtg
     3181 tatctttgaa tatgagattg ctcagacctg gcagtttctg agcagtcgtg ctggtatcat
     3241 cttcattgtc ctgcagaagg tggagaagac cctgctcagg cagcaggtgg agctgtaccg
     3301 ccttctcagc aggaacactt acctggagtg ggaggacagt gtcctggggc ggcacatctt
     3361 ctggagacga ctcagaaaag ccctgctgga tggtaaatca tggaatccag aaggaacagt
     3421 gggtacagga tgcaattggc aggaagcaac atctatcttg gacccagctt tcttgtacaa
     3481 agtggttgat ctcgaggact acaaggacga cgatgacaag ggagggggtg gatctggagg
     3541 gggtggatct atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     3601 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     3661 tgccacctac ggcaagctga ccctgaagct gatctgcacc accggcaagc tgcccgtgcc
     3721 ctggcccacc ctcgtgacca ccctgggcta cggcctgcag tgcttcgccc gctaccccga
     3781 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     3841 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     3901 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     3961 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca ccgccgacaa
     4021 gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt
     4081 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     4141 cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga
     4201 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     4261 gtacaagtaa ggtaccggtt agtaatgagt ttaaacgggg gaggctaact gaaacacgga
     4321 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
     4381 acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
     4441 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
     4501 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
     4561 gccatagcag atctgcgcag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     4621 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     4681 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     4741 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     4801 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     4861 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     4921 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     4981 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     5041 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     5101 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     5161 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     5221 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     5281 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     5341 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     5401 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     5461 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     5521 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     5581 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     5641 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     5701 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     5761 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     5821 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     5881 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     5941 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     6001 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     6061 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     6121 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     6181 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     6241 gactctgggg ttcgcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     6301 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     6361 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     6421 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6481 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     6541 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     6601 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     6661 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     6721 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     6781 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     6841 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     6901 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     6961 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     7021 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7081 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     7141 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     7201 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     7261 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     7321 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     7381 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     7441 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     7501 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     7561 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     7621 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     7681 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     7741 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     7801 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     7861 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     7921 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     7981 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     8041 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     8101 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     8161 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     8221 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     8281 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     8341 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     8401 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     8461 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     8521 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     8581 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     8641 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     8701 gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
