LOCUS       sequence-391876-        6741 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6741)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 6741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BNRF1
FEATURES             Location/Qualifiers
     source          1..6741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4620..4719)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4736..4755)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4737..4755)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4760..4776)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4760..4776)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5207..5998
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6091..6679
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6580..6599
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg gaagagaggg gcagggaaac gcaaatgccg gttgcccggt atgggggccc
      721 gtttattatg gtaaggctct tcgggcaaga tggagaggca aacatacagg aggaaaggct
      781 atatgagcta ctctctgacc cacgctccgc gctcggccta gacccggggc ccctgattgc
      841 tgagaacctg ctgctagtgg cgctgcgtgg caccaacaac gatcccaggc ctcagcgtca
      901 ggagagggcc agagaactgg ccctcgttgg cattctacta ggaaacggcg agcagggtga
      961 acacttgggc acggagagtg ccctggaggc ctcaggcaac aactatgtgt atgcctacgg
     1021 accagactgg atggcaaggc cttccacatg gtccgcggaa atccagcaat tcctgcgact
     1081 cctgggcgcc acgtacgtgc ttcgcgtgga gatgggcagg cagtttggct tcgaggtgca
     1141 tagaagccgg ccctccttcc gtcagttcca ggccatcaat caccttgtcc tgtttgacaa
     1201 cgcccttcgc aagtacgatt ccggccaggt ggcggcgggc ttccagaggg cccttctggt
     1261 ggccgggcca gagaccgctg acacgaggcc ggacctccgc aagctgaatg agtgggtgtt
     1321 tggtggcagg gctgctggtg gcagacagct ggccgacgag ctaaagatcg tgtccgcgct
     1381 gcgagacact tactcgggcc acttggtcct tcagcccacg gagacccttg acacatggaa
     1441 ggtgttgagc agggacacac gaaccgctca tagtttggag cacggattca ttcatgccgc
     1501 ggggaccatc caggccaact gcccacagct gtttatgaga cgccagcacc ccggcctctt
     1561 tcccttcgtt aatgcaatag catcatcgct gggctggtac taccagaccg ccaccggccc
     1621 cggagcagat gccagggcgg cggcccggcg ccaacaggcc tttcagacca gggcggcggc
     1681 tgaatgccat gccaaaagcg gggtgccggt cgtggccggc ttctacagga ccatcaacgc
     1741 cacgctcaag ggaggagagg gcctacagcc cactatgttt aacggggagc tgggggccat
     1801 caagcaccag gcacttgaca ctgtgaggta tgactacggc cactatctca taatgttggg
     1861 gccattccag ccatggagcg gactgacggc ccctccgtgc ccctacgccg aaagttcatg
     1921 ggcacaggcg gccgtgcaga cggccctcga gctgttctcg gccctgtacc cggccccgtg
     1981 catctcgggc tacgcgcgcc ccccgggccc cagtgctgtg atcgagcatc tggggtccct
     2041 agttccaaag gggggtctgc tgttgtttct gtctcaccta ccggatgatg ttaaggacgg
     2101 gctcggagaa atggggccgg ccagggccac gggacctgga atgcagcagt ttgtcagcag
     2161 ctacttcctc aaccccgcct gttccaacgt cttcattaca gtgaggcagc gaggggagaa
     2221 gatcaacggc cgtaccgtcc tccaagcgct cggacgcgca tgcgatatgg caggctgcca
     2281 gcactatgtg ctgggctcca cggttcccct cggtggactc aactttgtca acgacctggc
     2341 gtccccggtt tccaccgccg agatgatgga tgatttctct cccttcttca ccgtggagtt
     2401 tcccccgatt caagaggagg gcgcaagttc tccggtaccc ttagatgtgg acgagagcat
     2461 ggacatctct ccgtcttacg agttgccctg gctctcgctg gagtcatgcc tcacaagcat
     2521 cctgtcacac cccaccgtgg gaagcaagga gcacttggtc aggcacacgg acagggtcag
     2581 cggaggacgc gtggcacagc agcccggggt aggtcccctg gacctgccgc tggcggacta
     2641 cgccttcgtt gcccacagtc aggtctggac caggcccggt ggggctcctc ccttgcccta
     2701 tcgtacctgg gatcgaatga cagagaagct gcttgtctcc gcaaaacccg gcggagagaa
     2761 cgttaaggtt tcaggtaccg tgattacatt gggagaacag gggtacaaag tgtcgttgga
     2821 tctgagggag ggaaccaggc tggcaatggc tgaggcgctg ctgaacgcag catgtgcccc
     2881 aatcttggat ccggaagacg tcttgctcac cctgcatcta cacctggatc cgcgccgggc
     2941 agacaactcg gccgtgatgg aggctatgac ggcggcgagt gactacgcgc gtggcctggg
     3001 cgtgaagctg acctttggct cggcctcctg ccccgagacc ggctcgtccg cctccaactt
     3061 catgaccgtg gtggcctctg tctccgcccc aggggaattc tcgggtcctc tgatcacgcc
     3121 agtgcttcag aagacgggca gtctcctgat tgcggtgcgt tgcggggatg gcaagatcca
     3181 gggagggtcg ctgtttgagc agctctttag cgacgtggcc acgaccccac gggcacccga
     3241 ggcgttgtct ctgaagaatc tcttccgggc agtccagcag ctggtcaaga gcggcatcgt
     3301 gctgtcaggg catgacatca gcgacggggg cctggtgacc tgcctggtgg agatggccct
     3361 ggccgggcag cggggagtga ccatcactat gccggtggcc tccgactacc tcccggagat
     3421 gtttgcagag caccccggcc tggtgtttga ggtggaggag cgcagcgtgg gtgaggtgct
     3481 gcagaccctg cgctccatga acatgtaccc ggcagtcctc ggtcgagtgg gcgagcaagg
     3541 tccagatcaa atgtttgagg tgcagcacgg cccagagacg gtgttgcgcc agtcgctgcg
     3601 cctgctgctg ggaacctggt catcctttgc cagcgagcag tacgagtgcc tgcgaccaga
     3661 tcggattaac cggtccatgc acgtgtccga ctacggctat aacgaagcac tggcagtctc
     3721 cccgttgaca ggaaagaatc tcagcccacg ccggttggtg acagagcctg acccacgatg
     3781 tcaggtggcc gtgctatgcg ccccgggcac caggggccat gaaagcctcc tggcggcctt
     3841 cacgaatgcc ggatgcctgt gccgacgggt gttctttcgc gaggttaggg acaacacgtt
     3901 cctcgacaag tacgtgggtc tggccatcgg aggagttcat ggggccaggg actctgccct
     3961 ggcaggccgt gccaccgtgg cgctgattaa tcgtttcccc gccctgcgtg acgctattct
     4021 aaagttcctc aacaggccag atacgttctc ggtggccttg ggggagctgg gggtgcaagt
     4081 tttggctggc ctgggggccg tggggtcaac agataatcca cccgcccctg gcgtggaagt
     4141 taatgtccag agatcacctc tgattctggc ccccaacgcc tctggcatgt ttgagtcccg
     4201 ctggctgaac attagcatcc cggcgaccac cagctctgtc atgctgcgtg gcctccgggg
     4261 ctgcgtcctg ccttgttggg tgcaaggctc gtgcctgggc ctgcaattta ctaacctcgg
     4321 gatgccatat gttttgcaga atgcccacca gatcgcctgc cacttccaca gcaatggcac
     4381 ggatgcctgg cgctttgcta tgaattatcc aagaaacccc acggagcagg gcaacattgc
     4441 agggctctgt tcacgcgatg gtcgtcatct ggctctcctg tgtgacccct cactttgtac
     4501 agacttttgg caatgggagc acattccccc cgcctttggg caccccacgg ggtgctcccc
     4561 ctggacactt atgtttcaag cagctcacct atggtcactc aggcacggtc gcccctccga
     4621 gccaactttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa
     4681 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg atatccccta
     4741 tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa
     4801 tctctgatgt tacattgcac aagataaaaa tatatcatca tgcctcctct agaccagcca
     4861 ggacagaaat gcctcgactt cgctgctacc caaggttgcc gggtgacgca caccgtggaa
     4921 acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa
     4981 gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     5041 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg
     5101 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     5161 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacattatga gggaagcggt
     5221 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     5281 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     5341 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     5401 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     5461 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     5521 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     5581 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     5641 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     5701 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     5761 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     5821 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     5881 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
     5941 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc
     6001 ctcgagccac ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc
     6061 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6121 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6181 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     6241 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6301 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6361 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6421 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6481 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6541 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6601 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6661 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6721 ctggcctttt gctcacatgt t
//
