LOCUS       sequence-391886-        6822 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drives specific expression in the PHA neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 6822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pWP022
FEATURES             Location/Qualifiers
     source          1..6822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3880..4029
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        4567..4671
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4672..4740,4741..5532)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4890..4909)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5703..6291
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6192..6211
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6445..6462
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6579..6600
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6615..6620,6621..6638,6639..6645)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6653..6669
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6658..6680
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6677..6693
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6677..6693
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggcctgcc tttccgttct actagaacac
       61 gaaaataaaa tatgtgtacg tttgccaaaa aactttcgag ataaaatgtc agaaaagcaa
      121 aaaggacttg gcagaaagaa gcttgagaac agcggggttt ccatatcgac aatcttgtta
      181 ccaagtcttt tacacctgct atgttatgtc tacatcatat tcccatcggc catgcgagag
      241 tgtgctggct gccccttgct taagctcgtt tcaaaaccaa aagtgtgccg ctggataatc
      301 tctactttat aattatagtg tttcctctac caaagtaata attcatcttc gttgaatgaa
      361 aatcgttatc agaatatagt tgaagctcct agaattttta aaaactaaat agatttccag
      421 aagtctctct gtttttgttg ggattagaaa gaggaccgaa ataataatgt gttccatata
      481 gccaattcca ttttctttct taaagttttc gctgaggatc tgagaagaaa ttgctcacat
      541 tgtcgggtga cagtcagtgt gttaaaaatt tggatttttt aataaaaaat gttttgtaac
      601 ctgtttgtcg agaaaattta tcgacatcat gattggttaa aaatatgact tgaaaagtta
      661 gaattaagtt ggattcatga aaatcagttt ttcaaaacgt agaatggtat ttgaacaatt
      721 gatttattat gccccagctc agacgttaac tcctttataa cgcataaatt caaaactgca
      781 accagagaat aagcatgcaa tgagagaaag tatacagtag acaaaggatg accagtacag
      841 taatccatca cattgtgctt ttccagaata tgctgcgaat acaaaaactg aaccgacgat
      901 tgtgctgaaa tttattaaaa ctattgacag acttgggaaa gcttatttca aaattgtact
      961 tttaaacttt ttcttttatt ttctaaaagt aatacaactg atcaaaagca gttattatgg
     1021 agacgcgctt ttcaaaatta atgtgcaaac acgctccacg gcccaatgaa gacattccgc
     1081 ccccaaaagt tgggtctcgt taggggttgg tggcgaaacc gtacattcaa tttttttttg
     1141 ataatttttg ccggtttcac ctgtttcctt tttgttaggt tttccgtaat tttcaatgtt
     1201 atttgtgtcg aaataatgtt gaaaatgcat gtgagtgtga aaagtgtaat aaggatttct
     1261 tcattggatc atcaattttt tatgaatacg ggtgaaaact gcaaaactaa tcgaaagcat
     1321 ttgaacttac ggtattgccg tcaatactta acgagaccta gggctgtgca accggacgtc
     1381 cggttttttc ggacgtccga aaaaccggac gccggacgca cccaacgtcc ggttttttcg
     1441 gacgcgtgtg cgtccggcgt ccgaaaaaac cggacgcttg tgcgtccggc gtccggtttt
     1501 cggacgcggg tgcgtccggt ttttttgttt tttgtttaca tctcagtgag actgagtaag
     1561 attgtaaaca aaaaacaaaa aaacaaattt tcggacgtcc gaaaaaccgg acgccggacg
     1621 tccgaattcg tccgaattcg tccgattgca cagccccatt ttggactata atggtgcgtc
     1681 caaagtgcaa agtgctcgtc gagacccaac tattgggggc ggagcgtttt catttgtcgt
     1741 gggacgcgct ttcacctcga tttcaacagg cgttgcgtcc cccctaatat ttaatgtttc
     1801 aaattccaaa aaacaagttc tcactatcta aaatgtttga cccgaagaaa aaatcacaaa
     1861 cctgacgaat attcctaatc tgcaaatgtt gagtagaatg aataaagcca tggattttga
     1921 ttttccttga acttgttcca taatacactc gaaacttccc tgaaggaaaa ttaaagcaga
     1981 attgacaatc agccagactg gaatccgttg atctactgga cattggttaa cttttactat
     2041 tccgattacc aggaggtaca caggaatcga tgaaaatatt agaaatatcg ctgaaatata
     2101 tttttagttt atccgttgat agtaattaca tctatcttta tctgaatgag aagcaatttt
     2161 tatatttttg aaaattcaat tggcaaactg ccgaattatc gaaaaaaaaa tggtatttta
     2221 ttcagaaatt tgaagcagtg atttaccacg atgtttaaaa tttcgttgaa aatttttttt
     2281 atttgttcga cttccaaacg aaaatattag aactcacaat acagcacaat agaacaatac
     2341 agcaacacgc gaatagttcc cgaaacattt tcttcttgca acaaaaaact gaaacttttc
     2401 tagttctaaa cttatctatt cattttatat acaagtaagt caggtgctcg ccccagattg
     2461 ataaggtggg tgcatctcga aaaagcagcg gaagaaactc gtattcagag gaacaatttt
     2521 tgtctgccac gatgtttgga atgtgctgat aaattttact caaaagtgat agagagttgt
     2581 taagatacca tttagacttc agtagacaac gttcaaaatt gtcgattatt ttttaaccta
     2641 acagtgaata ttgggaaaca tatagttata aaataatcca gtatcatgtg atttagatgt
     2701 ggcaataaca agtaggcaag taggtaggaa cttagatatg ggtaggcatg tgggaagtta
     2761 aactgtcgtc aattacttgc ctttatcgcc agtgaacata acacaaataa aagttcttta
     2821 aaatctatca aatgtaatgt tgccaattga attcaaaaga ttataaacta tttaatattt
     2881 attttcaaag atttcggagt tattgcatat acaatttata catccgtcaa tctctttcac
     2941 tttcgtgaca tatcaatcta atgggtaaat tgaatgtcta aaagtgatat tcaatcagaa
     3001 gcaagttgcc aggaaatcac caaaaaccca catgcttctc aattttcttc cttgttgtaa
     3061 ttccccataa taaaaatttc aattaatgct tattacattt tacatgagaa ttttgttttc
     3121 ggcttcatat agaaattcat agaatcattt ttggaataat tccggtccat aattacctga
     3181 ctaaatacgc gctttgatat ttggtatcac ataaaacaac cgagctaaaa ttacagccca
     3241 ctttattggc gcgcctctag aggatccccg ggattggcca aaggacccaa aggtatgttt
     3301 cgaatgatac taacataaca tagaacattt tcaggaggac ccttggctag gatgggccct
     3361 aaaaagaagc gtaaagctag catgaagctg ttgtcttcaa tggaacaagc ttgcgacata
     3421 tgccggttaa aaaaactcag gtgctcgaag gaaaagccca agtgtgccaa gtgtctgaag
     3481 aacaactcgg agtgccgtta ttctcccaag gctatgaggt ccccactgac cagggcgcat
     3541 ctgacagagg tagaatcaag gttggaaaaa ttggaagatt tgtttctgct catgtttcct
     3601 cgggaaaacc tggacagcat tttgaacatg gactctttgg acgaagtgaa agtaatgttg
     3661 aaacaattat atatgcagga ctatggtgac acggatgctg ctatggatag cttgacccct
     3721 atggaaaatg gcatgtctga agggccgaga aaacacatga agactgcgac atcatcgcca
     3781 aaagtggatg aagatggaag ccaaagtcag ttgagtgtat cgccggaatt cccgctgcag
     3841 tctggtggcg gaggggctcc taggggttcc ggacgggctg acgcattgga cgattttgat
     3901 ctggatatgc tgggaagtga cgccctcgat gattttgacc ttgacatgct tggttcggat
     3961 gcccttgatg actttgacct cgacatgctc ggcagtgacg cccttgatga tttcgacctg
     4021 gacatgctga ttaactaagg taccgaattc gctagccggc catacaagta atccggatga
     4081 tcgacgccaa cgtcgttgaa ttttcaaatt ttaaatactg aatatttgtt ttttttccta
     4141 ttatttattt attctctttg tgtttttttt cttgctttct aaaaaattaa ttcaatccaa
     4201 atctaaacat ttttttttct ctttccgtct cccaattcgt attccgctcc tctcatctga
     4261 acacaatgtg caagtttatt tatcttctcg ctttcatttc attaggacgt ggggggaatt
     4321 ggtggaaggg ggaaacacac aaaaggatga tggaaatgaa ataaggacac acaatatgca
     4381 acaacattca attcagaaat atggaggaag gtttaaaaga aaacataaaa atatatagag
     4441 gaggaaggaa aactagtaaa aaataagcaa agaaattagg cgaacgatga gaattgtcct
     4501 cgcttgggcc caaaaggcct tttttttttg gcgccgacgt caggtggcac ttttcgggga
     4561 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4621 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     4681 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     4741 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     4801 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4861 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     4921 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     4981 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     5041 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     5101 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     5161 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     5221 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     5281 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     5341 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     5401 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     5461 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     5521 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     5581 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5641 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5701 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5761 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5821 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     5881 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     5941 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6001 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6061 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6121 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6181 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6241 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6301 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     6361 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     6421 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     6481 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     6541 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     6601 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     6661 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct gtaagtttaa
     6721 acatgatctt actaactaac tattctcatt taaattttca gagcttaaaa atggctgaaa
     6781 tcactcacaa cgatggatac gctaacaact tggaaatgaa at
//
