LOCUS       sequence-392103-       11424 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral construct to stably express LYCHOS (GPR155) WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11424)
  AUTHORS   Shin HR, Citron YR, Wang L, Tribouillard L, Goul CS, Stipp R, 
            Sugasawa Y, Jain A, Samson N, Lim CY, Davis OB, Castaneda-Carpio D, 
            Qian M, Nomura DK, Perera RM, Park E, Covey DF, Laplante M, Evers 
            AS, Zoncu R
  TITLE     Lysosomal GPCR-like protein LYCHOS signals cholesterol sufficiency 
            to mTORC1.
  JOURNAL   Science. 2022 Sep 16;377(6612):1290-1298. doi: 
            10.1126/science.abg6621. Epub 2022 Aug 25.
  PUBMED    36007018
REFERENCE   2  (bases 1 to 11424)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abg6621"; journalName: "Science"; date: 
            "2022-09-16- 16"; volume: "377"; issue: "6612"; pages: "1290-1298"
COMMENT     Sequence Label: pLVX LYCHOS (GPR155) WT-FLAG
FEATURES             Location/Qualifiers
     source          1..11424
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2337..3519
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2568..3510
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3467..3487
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             complement(5475..5486)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             6205..6228
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        6259..6758
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6294..6317)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6691..6710
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6779..7378
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6779..6798)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7275..7295
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7392..7980
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7445..7465)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7863..7874)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8187..8820
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8949..8965)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8949..8965)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8962..8984)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8973..8989
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8997..9003,9004..9021,9022..9027))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9042..9063
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9180..9197)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9351..9939)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9431..9450)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10110..10901,10902..10970))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10733..10752
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10971..11075)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11123..11257
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11160..11179)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11214..11233
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatcgctagc gctaccggac
     3541 tcagatctcg agctcaagct tcgaattgga tccgccacca tgaattctaa tttacctgca
     3601 gagaacttaa ccattgcagt caatatgacc aagactttgc ctacagcagt aacgcatgga
     3661 tttaattcca ctaatgaccc accttcaatg tcaattacaa ggctttttcc agccttactg
     3721 gaatgctttg gcattgtcct ttgtggctac atagcaggaa gggccaatgt cataacatca
     3781 acccaggcca aaggactagg aaattttgtc tccagatttg cacttccagc tttattattc
     3841 aaaaacatgg ttgtacttaa tttttccaat gtggactggt ccttcctata tagtatctta
     3901 attgccaaag cttctgtatt tttcattgta tgtgtattaa ccttattggt tgccagtcct
     3961 gatagtcgat ttagcaaagc tggactattc cctatttttg ctacacaaag taatgacttt
     4021 gcattgggat accctatagt tgaagcttta tatcaaacta catacccaga atatctccag
     4081 tacatttatt tggtggcacc aatatctctt atgatgttaa accctatagg gtttattttc
     4141 tgtgaaatcc aaaagtggaa agacactcaa aatgcttctc aaaataaaat aaaaattgtg
     4201 ggactcggac tcctgcgtgt attacagaac ccaatagtat ttatggtctt cattggcatc
     4261 gccttcaatt ttattcttga tcgaaaggta cctgtatatg tcgaaaattt tcttgatgga
     4321 cttggaaatt ctttttctgg atcagcccta ttttatcttg gtctcacgat ggtgggaaaa
     4381 ataaagagac tgaagaagtc ggcatttgta gtactaattc ttctcatcac agctaaactt
     4441 ctggtgctgc cacttctgtg cagagaaatg gtggaactct tggacaaggg cgacagtgtg
     4501 gtgaaccata caagtttatc aaattatgca tttctgtatg gtgtatttcc tgtagcacca
     4561 ggagtggcta tctttgcaac acaattcaac atggaagtag aaattataac ctcagggatg
     4621 gtgataagca catttgtgtc tgctcccatc atgtacgttt ctgcctggtt actgaccttt
     4681 cccactatgg accctaagcc attggcatat gccatccaga atgttagttt tgatataagt
     4741 attgtcagcc tgatctcctt gatctggtct ctggctattc ttcttttgag taagaaatat
     4801 aaacagcttc ctcatatgct tacaactaat ttactcattg ctcagtctat tgtctgtgct
     4861 ggaatgatga tatggaattt tgttaaagaa aaaaattttg ttggacaaat tttggtgttt
     4921 gttctattgt acagctccct ctatagcacc tacctgtgga caggccttct agcaatttct
     4981 ttgtttcttt tgaaaaagcg agagagggta caaattcctg ttggaataat cataatatct
     5041 ggctggggaa ttcctgctct ccttgttggt gttcttttga taactggaaa acacaatgga
     5101 gatagcattg actcagcctt cttttatgga aaagaacaga tgatcaccac agcagtcacc
     5161 ctgttctgca gcatcctgat agctggcata tccctcatgt gcatgaacca gactgcccaa
     5221 gcaggaagct atgaaggttt cgatcagtct cagagccaca aagtggtgga gcctggaaat
     5281 actgcttttg aggagagtcc agcaccagta aatgaaccag aactttttac aagctctatt
     5341 ccagaaacaa gttgctgctc ctgctccatg ggaaatggtg aattacactg cccatcaata
     5401 gagccaatag caaacacaag caccagtgag cctgtgattc cttcgtttga gaaaaacaat
     5461 cattgtgtga gtcgctgtaa ctcccagagc tgcatattag cccaggaaga agaacagtat
     5521 ctacagagtg gagaccagca actgacccga catgtgttgc tgtgtttact tctcatcatt
     5581 ggcctgttcg ctaatctttc cagttgttta tggtggctat tcaaccaaga gcctggaaga
     5641 ctttatgttg agttacagtt tttctgtgcc gtgtttaact ttggtcaggg atttatttcc
     5701 tttggaatct ttggattaga taaacattta atcatcctgc ctttcaaaag aagacttgaa
     5761 ttcctatgga acaataaaga cacagcagaa aacagggatt ctcctgtttc agaggaaata
     5821 aaaatgacct gtcaacaatt tatccattat caccgtgacc tctgtatccg aaacattgtc
     5881 aaagaaagaa ggtgtggtgc aaagacttct gctggaactt tctgtggctg tgacctggtg
     5941 agctggctaa ttgaagtcgg ccttgcctcc gaccgtggtg aagctgtgat atacggagac
     6001 aggctggtac aagggggagt catccaacat attaccaatg agtatgaatt ccgggatgag
     6061 tacttgtttt acagatttct tcaaaagagt cctgaacaga gtcctcctgc tattaatgca
     6121 aacactctcc aacaggaaag atataaagaa attgagcatt catccccacc ctcacattcc
     6181 cctaagaccg gtggtggtgg tggtgactac aaggatgacg atgacaagta agcggccgcg
     6241 actctagata attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     6301 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     6361 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     6421 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     6481 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     6541 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     6601 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggnnggctc aggggcgggg
     6661 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     6721 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat
     6781 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     6841 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     6901 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     6961 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     7021 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     7081 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     7141 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     7201 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     7261 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     7321 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacc
     7381 gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     7441 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     7501 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     7561 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     7621 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     7681 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     7741 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     7801 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     7861 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     7921 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     7981 ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     8041 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     8101 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     8161 ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga
     8221 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
     8281 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     8341 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
     8401 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
     8461 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
     8521 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
     8581 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
     8641 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     8701 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     8761 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     8821 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     8881 agagtgagag gccttgacat tgctagcgtt ttaccgtcga cctctagcta gagcttggcg
     8941 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     9001 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     9061 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     9121 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     9181 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     9241 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     9301 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     9361 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     9421 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     9481 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     9541 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     9601 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     9661 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     9721 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     9781 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     9841 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     9901 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     9961 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    10021 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    10081 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    10141 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    10201 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    10261 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    10321 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    10381 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    10441 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    10501 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    10561 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    10621 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    10681 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    10741 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    10801 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    10861 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    10921 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    10981 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    11041 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    11101 gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag
    11161 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    11221 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
    11281 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
    11341 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
    11401 aatatgtact acaaacttag tagt
//
