LOCUS       sequence-392154-        1691 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 2 part for Modular Cloning in Yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Simakin P, Koch C, Herrmann JM
  TITLE     A modular cloning (MoClo) toolkit for reliable intracellular protein
            targeting in the yeast Saccharomyces cerevisiae.
  JOURNAL   Microb Cell. 2023 Feb 28;10(4):78-87. doi: 10.15698/mic2023.04.794. 
            eCollection 2023 Apr 3.
  PUBMED    37009624
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15698/mic2023.04.794"; journalName: "Microb Cell"; date: 
            "2023-02-28- 28"; volume: "10"; issue: "4"; pages: "78-87"
COMMENT     Sequence Label: pHHYTK157
FEATURES             Location/Qualifiers
     source          1..1691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        14..32
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     14..31
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      complement(56..90)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(161..820)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     735..754
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1002..1590
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1491..1510
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgatttagg tgacactata gaagccacta tgtgagacca gaccaataaa
       61 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
      121 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact
      181 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
      241 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
      301 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
      361 tgaaactcac ccagggattg gctgaaacga aaaacatatt ctcaataaac cctttaggga
      421 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
      481 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
      541 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
      601 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
      661 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
      721 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
      781 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
      841 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
      901 tggaacctct tacgtgcccg atcaatcatg accaaaatcc cttaacgtga gttttcgttc
      961 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1021 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1081 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1141 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1201 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1261 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1321 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1381 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1441 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1501 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1561 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1621 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1681 gataaccgta g
//
