LOCUS       sequence-392157-        1959 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 3a part for Modular Cloning in Yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Simakin P, Koch C, Herrmann JM
  TITLE     A modular cloning (MoClo) toolkit for reliable intracellular protein
            targeting in the yeast Saccharomyces cerevisiae.
  JOURNAL   Microb Cell. 2023 Feb 28;10(4):78-87. doi: 10.15698/mic2023.04.794. 
            eCollection 2023 Apr 3.
  PUBMED    37009624
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15698/mic2023.04.794"; journalName: "Microb Cell"; date: 
            "2023-02-28- 28"; volume: "10"; issue: "4"; pages: "78-87"
COMMENT     Sequence Label: pHHYTK125
FEATURES             Location/Qualifiers
     source          1..1959
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(324..358)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(429..1088)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1003..1022
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1270..1858
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1759..1778
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat atgaagagct tcattacaag gaacaagaca gccattttgg caaccgttgc
       61 tgctacaggt actgccatcg gtgcctacta ttattacaac caattgcaac aacaacaaca
      121 acgaggaaaa aagaacacga tcaacaaaga tgaaaaaaag gacacaaagg actctcaaaa
      181 ggagactgaa ggtgctaaga aatctacagc cccatcaaat cctcctatct acccggtttc
      241 tagtaatggc gaaccagatt tttccaataa ggcaaatttc accgctgaag aaaaggataa
      301 atatggttct tgagaccaga ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca
      361 aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg
      421 atatcaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
      481 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
      541 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
      601 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgaaacgaaa
      661 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
      721 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
      781 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
      841 accagctcac cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca
      901 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
      961 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     1021 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     1081 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     1141 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgcccgat caatcatgac
     1201 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1261 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1321 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1381 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     1441 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1501 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1561 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1621 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     1681 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     1741 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     1801 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     1861 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     1921 ctttcctgcg ttatcccctg attctgtgga taaccgtag
//
