LOCUS       sequence-392225-       11759 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11759)
  AUTHORS   Frosst P, Guan T, Subauste C, Hahn K, Gerace L
  TITLE     Tpr is localized within the nuclear basket of the pore complex and 
            has a role in nuclear protein export.
  JOURNAL   J Cell Biol. 2002 Feb 18;156(4):617-30. Epub 2002 Feb 11.
  PUBMED    11839768
REFERENCE   2  (bases 1 to 11759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2002-02-18"; volume: "156(4)"; pages: "617-30. Epub 
            2002 Feb 11"
COMMENT     Sequence Label: EGFP-Tpr
FEATURES             Location/Qualifiers
     source          1..11759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      560..569
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      7639..7648
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7645..7647,7648..7650,7651..8364)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7645..7647,7648..7650,7651..8361)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7690..7711)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7951..7970)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8298..8319
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8487..8608
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8524..8543)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8578..8597
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8615..9070)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8752..8773)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8964..8983
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9097..9201
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9203..9560
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9203..9394
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9226..9246)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9411..9546
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9595..10389
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9595..10389
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9649..9668)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10259..10278
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10577..10596)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10621..10668
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10997..11585
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11486..11505
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgacac catggcggcg gtgttgcagc aagtcctgga gcgcacggag
      601 ctgaacaagc tgcccaagtc tgtccagaac aaacttgaaa agttccttgc tgatcagcaa
      661 tccgagatcg atggcctgaa ggggcggcat gagaaattta aggtggagag cgaacaacag
      721 tattttgaaa tagaaaagag gttgtcccac agtcaggaga gacttgtgaa tgaaacccga
      781 gagtgtcaaa gcttgcggct tgagctagag aaactcaaca atcaactgaa ggcactaact
      841 gagaaaaaca aagaacttga aattgctcag gatcgcaata ttgccattca gagccaattt
      901 acaagaacaa aggaagaatt agaagctgag aaaagagact taattagaac caatgagaga
      961 ctatctcaag aacttgaata cttaacagag gatgttaaac gtctgaatga aaaacttaaa
     1021 gaaagcaata caacaaaggg tgaacttcag ttaaaattgg atgaacttca agcttctgat
     1081 gtttctgtta agtatcgaga aaaacgcttg gagcaagaaa aggaattgct acatagtcag
     1141 aatacatggc tgaatacaga gttgaaaacc aaaactgatg aacttctggc tcttggaaga
     1201 gaaaaaggga atgagattct agagcttaaa tgtaatcttg aaaataaaaa agaagaggtt
     1261 tctagactgg aagaacaaat gaatggctta aaaacatcaa atgaacatct tcaaaagcat
     1321 gtggaggatc tgttgaccaa attaaaagag gccaaggaac aacaggccag tatggaagag
     1381 aaattccaca atgaattaaa tgcccacata aaactttcta atttgtacaa gagtgccgct
     1441 gatgactcag aagcaaagag caatgaacta acccgggcag tagaggaact acacaaactt
     1501 ttgaaagaag ctggtgaagc caacaaagca atacaagatc atcttctaga ggtggagcaa
     1561 tccaaagatc aaatggaaaa agaaatgctt gagaaaatag ggagattgga gaaggaatta
     1621 gagaatgcaa atgaccttct ttctgccaca aaacgtaaag gagccatatt gtctgaagaa
     1681 gagcttgccg ccatgtctcc tactgcagca gctgtagcta agatagtgaa acctgggatg
     1741 aaactaactg agctctataa tgcttatgtg gaaactcagg atcagttgct tttggagaaa
     1801 ctagagaaca aaagaattaa taagtaccta gatgaaatag tgaaagaagt ggaagccaaa
     1861 gcaccaattt tgaaacgcca gcgtgaggaa tatgaacgtg cacagaaagc tgtagcaagt
     1921 ttatctgtta agcttgaaca agctatgaag gagattcagc gattgcagga ggacactgat
     1981 aaagccaaca agcaatcatc tgtacttgag agagataatc gaagaatgga aatacaagta
     2041 aaagatcttt cacaacagat tagagtgctt ttgatggaac ttgaagaagc aaggggtaac
     2101 cacgtaattc gtgatgagga agtaagctct gctgatataa gtagttcatc tgaggtaata
     2161 tcacagcatc tagtatctta cagaaatatt gaagagcttc aacaacaaaa tcaacgtctc
     2221 ttagtggccc ttagagagct tggggaaacc agagaaagag aagaacaaga aacaacttca
     2281 tccaaaatca ctgagcttca gctcaaactt gagagtgccc ttactgaact agaacaactc
     2341 cgcaaatcac gacagcatca aatgcagctt gttgattcca tagttcgtca gcgtgatatg
     2401 taccgtattt tattgtcaca aacaacagga gttgccattc cattacatgc ttcaagctta
     2461 gatgatgttt ctcttgcatc aactccaaaa cgtccaagta catcacagac tgtttccact
     2521 cctgctccag tacctgttat tgaatcaaca gaggctatag aggctaaggc tgcccttaaa
     2581 cagttgcagg aaatttttga gaactacaaa aaagaaaaag cagaaaatga aaaaatacaa
     2641 aatgagcagc ttgagaaact tcaagaacaa gttacagatt tgcgatcaca aaataccaaa
     2701 atttctaccc agctagattt tgcttctaaa cgttatgaaa tgctgcaaga taatgttgaa
     2761 ggatatcgtc gagaaataac atcacttcat gagagaaatc agaaactcac tgccacaact
     2821 caaaagcaag aacagattat caatacgatg actcaagatt tgagaggagc aaatgagaag
     2881 ctagctgtcg cagaagtaag agcagaaaat ttgaagaagg aaaaggaaat gcttaaattg
     2941 tctgaagttc gtctttctca gcaaagagag tctttgttag ctgaacaaag ggggcaaaac
     3001 ttactgctaa ctaatctgca aacaattcag ggaatactgg agcgatctga aacagaaacc
     3061 aaacaaaggc ttagtagcca gatagaaaaa ctggaacatg agatctctca tctaaagaag
     3121 aagttggaaa atgaggtgga acaaaggcat acacttacta gaaatctaga tgttcaactt
     3181 ttagatacaa agagacaact ggatacagag acaaatcttc atcttaacac aaaagaacta
     3241 ttaaaaaatg ctcaaaaaga aattgccaca ttgaaacagc acctcagtaa tatggaagtc
     3301 caagttgctt ctcagtcttc acagagaact ggtaaaggtc agcctagcaa caaagaagat
     3361 gtggatgatc ttgtgagtca gctaagacag acagaagagc aggtgaatga cttaaaggag
     3421 agactcaaaa caagtacgag caatgtggaa caatatcaag caatggttac tagtttagaa
     3481 gaatccctga acaaggaaaa acaggtgaca gaagaagtgc gtaagaatat tgaagttcgt
     3541 ttaaaagagt cagctgaatt tcagacacag ttggaaaaga agttgatgga agtagagaag
     3601 gaaaaacaag aacttcagga tgataaaaga agagccatag agagcatgga acaacagtta
     3661 tctgaattga agaaaacact ttctagtgtt cagaatgaag tacaagaagc tcttcagaga
     3721 gcaagcacag ctttaagtaa tgagcagcaa gccagacgtg actgtcagga acaagctaaa
     3781 atagctgtgg aagctcagaa taagtatgag agagaattga tgctgcatgc tgctgatgtt
     3841 gaagctctac aagctgcgaa ggagcaggtt tcaaaaatgg catcagtccg tcagcatttg
     3901 gaagaaacaa cacagaaagc agaatcacag ttgttggagt gtaaagcatc ttgggaggaa
     3961 agagagagaa tgttaaagga tgaagtttcc aaatgtgtat gtcgctgtga agatctggag
     4021 aaacaaaaca gattacttca tgatcagatc gaaaaattaa gtgacaaggt cgttgcctct
     4081 gtgaaggaag gtgtacaagg tccactgaat gtatctctca gtgaagaagg aaaatctcaa
     4141 gaacaaattt tggaaattct cagatttata cgacgagaaa aagaaattgc tgaaactagg
     4201 tttgaggtgg ctcaggttga gagtctgcgt tatcgacaaa gggttgaact tttagaaaga
     4261 gagctgcagg aactgcaaga tagtctaaat gctgaaaggg agaaagtcca ggtaactgca
     4321 aaaacaatgg ctcagcatga agaactgatg aagaaaactg aaacaatgaa tgtagttatg
     4381 gagaccaata aaatgctaag agaagagaag gagagactag aacaggatct acagcaaatg
     4441 caagcaaagg tgaggaaact ggagttagat attttaccct tacaagaagc aaatgctgag
     4501 ctgagtgaga aaagcggtat gttgcaggca gagaagaagc tcttagaaga ggatgtcaaa
     4561 cgttggaaag cacgtaacca gcatctagta agtcaacaga aagatccaga tacagaagaa
     4621 tatcggaagc tcctttctga aaaggaagtt catactaagc gtattcaaca attgacagaa
     4681 gaaattggta gacttaaagc tgaaattgca agatcaaatg catctttgac taacaaccag
     4741 aacttaattc agagtctgaa ggaagatcta aataaagtaa gaactgaaaa ggaaaccatc
     4801 cagaaggact tagatgccaa aataattgat atccaagaaa aagtcaaaac tattactcaa
     4861 gttaagaaaa ttggacgtag gtacaagact caatatgaag aacttaaagc acaacaggat
     4921 aaggttatgg agacatcggc tcagtcctct ggagaccatc aggagcagca tgtttcagtc
     4981 caggaaatgc aggaactcaa agaaacgctc aaccaagctg aaacaaaatc aaaatcactt
     5041 gaaagtcaag tagagaatct gcagaagaca ttatctgaaa aagagacaga agcaagaaat
     5101 ctccaggaac agactgtgca acttcagtct gaactttcac gacttcgtca ggatcttcaa
     5161 gatagaacca cacaggagga gcagctccga caacagataa ctgaaaagga agaaaaaacc
     5221 agaaaggcta ttgtagcagc aaagtcaaaa attgcacact tagctggtgt aaaagatcag
     5281 ctaactaaag aaaatgagga gcttaaacaa aggaatggag ccttagatca gcagaaagat
     5341 gaattggatg ttcgcattac tgcgctaaag tcccaatatg aaggtcgaat tagtcgcttg
     5401 gaaagagaac tcagggagca tcaagagaga caccttgagc agagagatga gcctcaagaa
     5461 ccttctaata aggtccctga acagcagaga cagatcacat tgaaaacaac tccagcttct
     5521 ggtgaaagag gaattgccag cacatcagac ccaccaacag ccaatatcaa gccaactcct
     5581 gttgtgtcta ctccaagtaa agtgacagct gcagctatgg ctggaaataa gtcaacaccc
     5641 agggctagta tccgcccaat ggttacacct gcaactgtta caaatcccac tactacccca
     5701 acagctacag tgatgcccac tacacaagtg gaatcacagg aagctatgca gtcagaaggg
     5761 cctgtggaac atgttccagt ttttggaagc acaagtggat ccgttcgttc tactagtcct
     5821 aatgtccagc cttctatctc tcaacctatt ttaactgttc agcaacaaac acaggctaca
     5881 gcttttgtgc aacccactca acagagtcat cctcagattg agcctgccaa tcaagagtta
     5941 tcttcaaaca tagtagaggt tgttcagagt tcaccagttg agcggccttc tacttccaca
     6001 gcagtatttg gcacagtttc ggctaccccc agttcttctt tgccaaagcg tacacgtgaa
     6061 gaggaagagg atagcaccat agaagcatca gaccaagtct ctgatgatac agtggaaatg
     6121 cctcttccaa agaagttgaa aagtgtcaca cctgtaggaa ctgaggaaga agttatggca
     6181 gaagaaagta ctgatggaga ggtagagact caggtataca accaggattc tcaagattcc
     6241 attggagaag gagttaccca gggagattat acacctatgg aagacagtga agaaacctct
     6301 cagtctctac aaatagatct tgggccactt caatcagatc agcagacgac aacttcatcc
     6361 caggatggtc aaggcaaagg agatgatgtc attgtaattg acagtgatga tgaagaagag
     6421 gatgaggaag atgatgatga tgatgaagat gacacaggga tgggagatga gggtgaagat
     6481 agtaatgaag gaactggtag tgccgatggc aatgatggtt atgaagctga tgatgctgag
     6541 ggtggtgatg ggactgatcc aggtacagaa acagaagaaa gtatgggtgg aggtgaaggt
     6601 aatcacagag ctgctgattc tcaaaacagt ggtgaaggaa atacaggtgc tgcagaatct
     6661 tctttttctc aggaggtttc tagagaacaa cagccatcat cagcatctga aagacaggcc
     6721 cctcgagcac ctcagtcacc gagacgccca ccacatccac ttcccccaag actgaccatt
     6781 catgccccac ctcaggagtt gggaccacca gttcagagaa ttcagatgac ccgaaggcag
     6841 tctgtaggac gtggccttca gttgactcca ggaataggtg gcatgcaaca gcattttttt
     6901 gatgatgaag acagaacagt tccaagtact ccaactcttg tggtgccaca tcgtactgat
     6961 ggatttgctg aagcaattca ttcgccgcag gttgctggtg tccctagatt ccggtttggg
     7021 ccacctgaag atatgccaca aacaagttct agtcactctg atcttggcca gcttgcttct
     7081 caaggaggtt taggaatgta tgaaacaccc ctgttcctag ctcatgaaga agagtcaggt
     7141 ggccgaagtg ttcccactac tccactacaa gtagcagccc cagtgactgt atttactgag
     7201 agcaccacct ctgatgcttc ggaacatgcc tctcaatctg ttccaatggt gactacatcc
     7261 actggcactt tatctacaac aaatgaaaca gcaacaggtg atgatggaga tgaagtattt
     7321 gtggaggcag aatctgaagg tattagttca gaagcaggcc tagaaattga tagccagcag
     7381 gaagaagagc cggttcaagc atctgatgag tcagatctcc cctccaccag ccaggatcct
     7441 ccttctagct catctgtaga tactagtagt agtcaaccaa agcctttcag acgagtaaga
     7501 cttcagacaa cattgagaca aggtgtccgt ggtcgtcagt ttaacagaca gagaggtgtg
     7561 agccatgcaa tgggagggag aggaggaata aacagaggaa atattaatgc ggtaccgcgg
     7621 gcccgggatc caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
     7681 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     7741 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     7801 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     7861 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     7921 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     7981 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     8041 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     8101 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     8161 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     8221 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     8281 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     8341 ggcatggacg agctgtacaa gtaaagcggc cgcgactcta gatcataatc agccatacca
     8401 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     8461 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     8521 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     8581 gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta
     8641 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     8701 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     8761 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     8821 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     8881 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     8941 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     9001 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     9061 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     9121 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     9181 taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt
     9241 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     9301 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     9361 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     9421 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     9481 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     9541 gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt
     9601 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     9661 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     9721 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac
     9781 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     9841 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     9901 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     9961 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
    10021 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
    10081 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag
    10141 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
    10201 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
    10261 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
    10321 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
    10381 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
    10441 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
    10501 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
    10561 ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
    10621 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg
    10681 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg
    10741 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
    10801 cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga
    10861 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    10921 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    10981 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    11041 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    11101 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    11161 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    11221 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    11281 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    11341 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    11401 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    11461 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    11521 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    11581 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    11641 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcatt
    11701 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccg
//
