LOCUS       sequence-39224-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homologous recombination plasmid for gene targeting at the mouse 
            Hprt locus containing Ple32 (CLDN5 MiniPromoter) driving a lacZ 
            reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   de Leeuw CN, Dyka FM, Boye SL, Laprise S, Zhou M, Chou AY, Borretta 
            L, McInerny SC, Banks KG, Portales-Casamar E, Swanson MI, D'Souza 
            CA, Boye SE, Jones SJ, Holt RA, Goldowitz D, Hauswirth WW, Wasserman
            WW, Simpson EM
  TITLE     Targeted CNS Delivery Using Human MiniPromoters and Demonstrated 
            Compatibility with Adeno-Associated Viral Vectors.
  JOURNAL   Mol Ther Methods Clin Dev. 2014 Jan 8;1:5.
  PUBMED    24761428
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2014-01-8"; volume: "1"; pages: "5"
COMMENT     Sequence Label: pEMS1503
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..20
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aaataggcgt atcacgaggc cctttcgtct tcaagaatta attccgatca tattcaataa
       61 cccttaatga attcgagctc ctaggccggc ccttgccttc agaacctccc cacactaggt
      121 gagccagacg ctggccttat ctcatttacc atctcagagc catctgaagg gggagaaggg
      181 aaccgggccc caggagggag aaagtcatca aacctcccac atctgtgacc agcctcagtg
      241 ccatactttt tctatggagg gccctgtcca atggaactga gcacagacca gataaaagaa
      301 ctgggcaccc agtggcctca gtccagggcc tggagttcaa actttactgg aaacaaaggg
      361 gccgagagag actggggaaa gaactactag aaaggggctg gtgcccccat ggggctgtgg
      421 gttttggagc cgcgtgcccc cacctgagcc tcagggggcc cggagtgtcc acaccagtgg
      481 acctttcgag aaatggctgg gccattgtgc agaagaatgc ccggaaatcc cgcgcctccc
      541 tcctccagca aggatggggg ctcttcctcc tggccaggaa actccaagtt ggcttccgga
      601 gggtggcctg ggggctgggg tgccagggac accatcgcca ctggtgggag ggcagggcac
      661 agcccctccg tgtccctttg tctctcctgt ctgaaggcca gagcaggctg ctaggcctgg
      721 ggccaccact gcccctgggt gctacaccca gtgtgctggg tcactgggaa cttcctgaag
      781 tggtgtcacc tgaactgggc ccccaaggat ggggtgcggg c
//
