LOCUS       sequence-39233-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Minderer M, Liu W, Sumanovski LT, Kugler S, Helmchen F, Margolis DJ
  TITLE     Chronic imaging of cortical sensory map dynamics using a genetically
            encoded calcium indicator.
  JOURNAL   J Physiol. 2011 Nov 14.
  PUBMED    22083602
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Physiol. 
            2011 Nov 14."
COMMENT     Sequence Label: AAV-6P-SEW-YC3.6
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        22..469
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      495..504
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(546..567)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(807..826)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctgcagaggg ccctgcgtat gagtgcaagt gggttttagg accaggatga ggcggggtgg
       61 gggtgcctac ctgacgaccg accccgaccc actggacaag cacccaaccc ccattcccca
      121 aattgcgcat cccctatcag agagggggag gggaaacagg atgcggcgag gcgcgtgcgc
      181 actgccagct tcagcaccgc ggacagtgcc ttcgcccccg cctggcggcg cgcgccaccg
      241 ccgcctcagc actgaaggcg cgctgacgtc actcgccggt cccccgcaaa ctccccttcc
      301 cggccacctt ggtcgcgtcc gcgccgccgc cggcccagcc ggaccgcacc acgcgaggcg
      361 cgagataggg gggcacgggc gcgaccatct gcgctgcggc gccggcgact cagcgctgcc
      421 tcagtctgcg gtgggcagcg gaggagtcgt gtcgtgcctg agagcgcagt cgaattcaag
      481 ctgctagcgc aagtgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
      541 ccatcctggt cgagctggac ggcgacgtaa acggccacag gttcagcgtg tccggcgagg
      601 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      661 tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc
      721 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      781 tccaggagcg taccatcttc ttcaaggacg acggcaacta caagacc
//
