LOCUS       sequence-392478-       11217 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Generation of Expandable megakaryocyte cell lines from human iPS 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11217)
  AUTHORS   Nakamura S, Takayama N, Hirata S, Seo H, Endo H, Ochi K, Fujita K, 
            Koike T, Harimoto K, Dohda T, Watanabe A, Okita K, Takahashi N, 
            Sawaguchi A, Yamanaka S, Nakauchi H, Nishimura S, Eto K
  TITLE     Expandable megakaryocyte cell lines enable clinically applicable 
            generation of platelets from human induced pluripotent stem cells.
  JOURNAL   Cell Stem Cell. 2014 Apr 3;14(4):535-48. doi: 
            10.1016/j.stem.2014.01.011. Epub 2014 Feb 13.
  PUBMED    24529595
REFERENCE   2  (bases 1 to 11217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2014.01.011"; journalName: "Cell Stem Cell"; date: 
            "2014-04-3- 3"; volume: "14"; issue: "4"; pages: "535-48"
COMMENT     Sequence Label: CS-TRE-BMI1-Ubc-rtTA
FEATURES             Location/Qualifiers
     source          1..11217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(63..82)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        254..633
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        635..833
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             851..1031
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1078..1203
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1696..1929
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2114..2158
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2432..2549
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2591..2905
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2599..2617
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2635..2653
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2670..2688
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2706..2724
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2742..2760
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2777..2795
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2813..2831
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2878..2902
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        3937..5148
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4985..5004
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(5161..5778,5779..5784,5785..5820,5821..5823,5824..5859,
                     5860..5862,5863..5898,5899..5907)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5931..6519
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5984..6004)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6402..6413)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7038..7218
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7244..7261)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7250..7474
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7520..7948
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7607..7626)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7817..7838
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7957..7977)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7962..8291
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8142..8277
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8204..8223
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8339..8386
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8405..8779
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8909..9030
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8946..8965)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9000..9019
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9079..9095)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9079..9095)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9092..9114)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9103..9119
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9127..9133,9134..9151,9152..9157))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9172..9193
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9310..9327)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9481..10069)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9561..9580)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10240..11031,11032..11100))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10863..10882
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11101..11205)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc
       61 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
      121 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
      181 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
      241 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
      301 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      361 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      421 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      481 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      541 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      601 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      661 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      721 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      781 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt
      841 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
      901 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
      961 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     1021 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     1081 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     1141 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     1201 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     1261 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     1321 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     1381 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     1441 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     1501 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     1561 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     1621 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     1681 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     1741 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     1801 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     1861 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     1921 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     1981 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     2041 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     2101 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     2161 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     2221 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     2281 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     2341 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     2401 cggatctaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     2461 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     2521 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agaaattcac
     2581 tttgaattgc gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta
     2641 tcagtgatag agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag
     2701 tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga
     2761 acgtatgtcg agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc
     2821 agtgatagag aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc
     2881 tcgtttagtg aaccgtcaga tcgcctggag aattgcgtac ggccagcatg catcgaacaa
     2941 cgagaatcaa gatcactgag ctaaatcccc acctgatgtg tgtgctttgt ggagggtact
     3001 tcattgatgc cacaaccata atagaatgtc tacattcctt ctgtaaaacg tgtattgttc
     3061 gttacctgga gaccagcaag tattgtccta tttgtgatgt ccaagttcac aagaccagac
     3121 cactactgaa tataaggtca gataaaactc tccaagatat tgtatacaaa ttagttccag
     3181 ggcttttcaa aaatgaaatg aagagaagaa gggattttta tgcagctcat ccttctgctg
     3241 atgctgccaa tggctctaat gaagatagag gagaggttgc agatgaagat aagagaatta
     3301 taactgatga tgagataata agcttatcca ttgaattctt tgaccagaac agattggatc
     3361 ggaaagtaaa caaagacaaa gagaaatcta aggaggaagt gaatgataaa agatacttac
     3421 gatgcccagc agcaatgact gtgatgcact taagaaagtt tctcagaagt aaaatggaca
     3481 tacctaatac tttccagatt gatgtcatgt atgaggagga acctttaaag gattattata
     3541 cactaatgga tattgcctac atttatacct ggagaaggaa tggtccactt ccattgaaat
     3601 acagagttcg acctacttgt aaaagaatga agatcagtca ccagagagat ggactgacaa
     3661 atgctggaga actggaaagt gactctggga gtgacaaggc caacagccca gcaggaggta
     3721 ttccctccac ctcttcttgt ttgcctagcc ccagtactcc agtgcagtct cctcatccac
     3781 agtttcctca catttccagt actatgaatg gaaccagcaa cagccccagc ggtaaccacc
     3841 aatcttcttt tgccaataga cctcgaaaat catcagtaaa tgggtcatca gcaacttctt
     3901 ctggttgaac cggtctcgag aattcgctag cgatctggcc tccgcgccgg gttttggcgc
     3961 ctcccgcggg cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg
     4021 agcgtcctga tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg
     4081 ccttagaacc ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg
     4141 cactggtttt ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt
     4201 ctgcggaggg atctccgtgg ggcggtgaac gccgatgatt atataaggac gcgccgggtg
     4261 tggcacagct agttccgtcg cagccgggat ttgggtcgcg gttcttgttt gtggatcgct
     4321 gtgatcgtca cttggtgagt agcgggctgc tgggctggcc ggggctttcg tggccgccgg
     4381 gccgctcggt gggacggaag cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg
     4441 agcaaggttg ccctgaactg ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga
     4501 gtcttgaatg gaagacgctt gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc
     4561 atggtgggcg gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa agctcttatt
     4621 cgggtgagat gggctggggc accatctggg gaccctgacg tgaagtttgt cactgactgg
     4681 agaactcggt ttgtcgtctg ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc
     4741 acccgtacct ttgggagcgc gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct
     4801 tataatgcag ggtggggcca cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg
     4861 acgcagggtt cgggcctagg gtaggctctc ctgaatcgac aggcgccgga cctctggtga
     4921 ggggagggat aagtgaggcg tcagtttctt tggtcggttt tatgtaccta tcttcttaag
     4981 tagctgaagc tccggttttg aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt
     5041 ttttaggcac cttttgaaat gtaatcattt gggtcaatat gtaattttca gtgttagact
     5101 agtaaattgt ccgctaaatt ctggccgttt ttggcttttt tgttagacaa gcttgtcacc
     5161 atgtctagac tggacaagag caaagtcata aacggcgctc tggaattact caatggagtc
     5221 ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga gcagcctacc
     5281 ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat cgagatgctg
     5341 gacaggcatc atacccactt ctgccccctg gaaggcgagt catggcaaga ctttctgcgg
     5401 aacaacgcca agtcattccg ctgtgctctc ctctcacatc gcgacggggc taaagtgcat
     5461 ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg
     5521 tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt gggccacttt
     5581 acactgggct gcgtattgga ggaacaggag catcaagtag caaaagagga aagagagaca
     5641 cctaccaccg attctatgcc cccacttctg agacaagcaa ttgagctgtt cgaccggcag
     5701 ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgtggcct ggagaaacag
     5761 ctaaagtgcg aaagcggcgg gccggccgac gcccttgacg attttgactt agacatgctc
     5821 ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat
     5881 tttgaccttg acatgctccc cgggtaactc gagggatcaa gcttatcgat aatcaacctc
     5941 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     6001 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     6061 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     6121 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     6181 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     6241 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     6301 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     6361 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     6421 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     6481 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgagacctag
     6541 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
     6601 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
     6661 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     6721 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
     6781 aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga
     6841 cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg
     6901 aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     6961 gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc
     7021 tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     7081 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     7141 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     7201 tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     7261 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     7321 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     7381 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     7441 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     7501 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     7561 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     7621 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     7681 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     7741 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     7801 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     7861 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     7921 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     7981 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     8041 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     8101 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     8161 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     8221 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     8281 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt
     8341 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
     8401 aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc
     8461 ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc
     8521 cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc
     8581 ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc
     8641 ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga
     8701 gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt
     8761 ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga
     8821 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     8881 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     8941 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     9001 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     9061 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     9121 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     9181 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     9241 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     9301 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     9361 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     9421 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     9481 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9541 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     9601 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     9661 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     9721 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     9781 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     9841 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     9901 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     9961 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    10021 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    10081 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    10141 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    10201 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    10261 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    10321 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    10381 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    10441 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    10501 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    10561 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    10621 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    10681 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    10741 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    10801 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    10861 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    10921 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    10981 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    11041 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    11101 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    11161 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccg
//
