LOCUS       sequence-39255-e         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the eGFP reporter gene was not detected in the brain 
            and eye..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1169
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2..11
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accatggtgg cggaagttcc tatacctttt gaagaatagg aacttcggcg cgccccgagg
       61 acgcccttca ggaacgccct ggggtgggcc ggtcggcagg ccgcccctcc ccgcggacct
      121 gcgcgcgggg aaacgatggt ggggccctgg cccgcgccct acccctttaa acccggcttg
      181 ggaacctgcg gggcagcggg tggcggggcg cagccaatgg aggcgcgagg ccgggcgcag
      241 ccgcgtgttc attggctgct tgtgccgggg ccgcgcggag gtgcggctgg caccgcttta
      301 ggaggttatt gtgagcgcct gtgccagccg cgcgggacag gccccctgag cggagtaagg
      361 gcgccttttc aaggcaagcg gggctgttga aggaggactg gtcgcactgg tcaggctgca
      421 actcacgtgc cttcctccag caactgtcta ctcaaaaaca acctccctca tatttttttt
      481 taactgtgat cacatattca taacataaaa tttaccgtct tatgtggata tgctgcatat
      541 ttcagtgcct tggtagaggg cctcagaaga gggttctggg tggccatcca gctccagcct
      601 gggcacggga tttgggactg gttggagctg gtgacttgga tggtcccttg tgctgggctg
      661 aagcagcggc atgcaaagtg tgacagctgg caagggccag ccagttactg tggccgctgg
      721 cacgggcccc ggggtctgtc tcaaggggag gcagctgctg cggcctccca
//
