LOCUS       sequence-392618-       10118 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nedd4L in pQCXIN vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10118)
  AUTHORS   Qualls-Histed SJ, Nielsen CP, MacGurn JA
  TITLE     Lysosomal trafficking of the glucose transporter GLUT1 requires 
            sequential regulation by TXNIP and ubiquitin.
  JOURNAL   iScience. 2023 Feb 6;26(3):106150. doi: 10.1016/j.isci.2023.106150. 
            eCollection 2023 Mar 17.
  PUBMED    36890792
REFERENCE   2  (bases 1 to 10118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2023-02-6"; volume: "26(3):106150. doi"; pages: " 
            10.1016/j.isci.2023.106150. eCollection 2023 Mar 17"
COMMENT     Sequence Label: WT Nedd4L
FEATURES             Location/Qualifiers
     source          1..10118
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1604..1907
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1908..2111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2108..2132
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    join(5026..5600,5601..5603,5604..5612)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5193..5210)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5420..5439
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5613..6407
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5667..5686)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6277..6296
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             6824..7249
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(7352..7374)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7510..7530)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7515..7844
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7695..7830
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7866..7885
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7966..7983)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8137..8725)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8217..8236)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8896..9687,9688..9756))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9519..9538
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9757..9861)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9929..9947
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(10047..10118,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1921 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2221 cggacctgca ggaattgatc cgcggccgca atggcgaccg ggctcgggga gccggtctat
     2281 ggactttccg aagacgaggg agagtcccgt attctcagag taaaagttgt ttctggaatt
     2341 gatctcgcca aaaaggacat ctttggagcc agtgatccgt atgtgaaact ttcattgtac
     2401 gtagcggatg agaatagaga acttgctttg gtccagacaa aaacaattaa aaagacactg
     2461 aacccaaaat ggaatgaaga attttatttc agggtaaacc catctaatca cagactccta
     2521 tttgaagtat ttgacgaaaa tagactgaca cgagacgact tcctgggcca ggtggacgtg
     2581 ccccttagtc accttccgac agaagatcca accatggagc gaccctatac atttaaggac
     2641 tttctcctca gaccaagaag tcataagtct cgagttaagg gatttttgcg attgaaaatg
     2701 gcctatatgc caaaaaatgg aggtcaagat gaagaaaaca gtgaccagag ggatgacatg
     2761 gagcatggat gggaagttgt tgactcaaat gactcggctt ctcagcacca agaggaactt
     2821 cctcctcctc ctctgcctcc cgggtgggaa gaaaaagtgg acaatttagg ccgaacttac
     2881 tatgtcaacc acaacaaccg gaccactcag tggcacagac caagcctgat ggacgtgtcc
     2941 tcggagtcgg acaataacat cagacagatc aaccaggagg cagcacaccg gcgcttccgc
     3001 tcccgcaggc acatcagcga agacttggag cccgagccct cggagggcgg ggatgtcccc
     3061 gagccttggg agaccatttc agaggaagtg aatatcgctg gagactctct cggtctggct
     3121 ctgcccccac caccggcctc cccaggatct cggaccagcc ctcaggagct gtcagaggaa
     3181 ctaagcagaa ggcttcagat cactccagac tccaatgggg aacagttcag ctctttgatt
     3241 caaagagaac cctcctcaag gttgaggtca tgcagtgtca ccgacgcagt tgcagaacag
     3301 ggccatctac caccgcttgc agaagatggt gcgtccggat cagccacaaa cagtaacaac
     3361 catctaatcg agcctcagat ccgccggcct cgtagcctca gctcgccaac agtaacttta
     3421 tctgccccgc tggagggtgc caaggactca cccgtacgtc gggctgtgaa agacaccctt
     3481 tccaacccac agtccccaca gccatcacct tacaactccc ccaaaccaca acacaaagtc
     3541 acacagagct tcttgccacc cggctgggaa atgaggatag cgccaaacgg ccggcccttc
     3601 ttcattgatc ataacacaaa gactacaacc tgggaagatc cacgtttgaa atttccagta
     3661 catatgcggt caaagacatc tttaaacccc aatgaccttg gcccccttcc tcctggctgg
     3721 gaagaaagaa ttcacttgga tggccgaacg ttttatattg atcataatag caaaattact
     3781 cagtgggaag acccaagact gcagaaccca gctattactg gtccggctgt cccttactcc
     3841 agagaattta agcagaaata tgactacttc aggaagaaat taaagaaacc tgctgatatc
     3901 cccaataggt ttgaaatgaa acttcacaga aataacatat ttgaagagtc ctatcggaga
     3961 attatgtccg tgaaaagacc agatgtccta aaagctagac tgtggattga gtttgaatca
     4021 gagaaaggtc ttgactatgg gggtgtggcc agagaatggt tcttcttact gtccaaagag
     4081 atgttcaacc cctactacgg cctctttgag tactctgcca cggacaacta cacccttcag
     4141 atcaacccta attcaggcct ctgtaatgag gatcatttgt cctacttcac ttttattgga
     4201 agagttgctg gtctggccgt atttcatggg aagctcttag atggtttctt cattagacca
     4261 ttttacaaga tgatgttggg aaagcagata accctgaatg acatggaatc tgtggatagt
     4321 gaatattaca actctttgaa atggatcctg gagaatgacc ctactgagct ggacctcatg
     4381 ttctgcatag acgaagaaaa ctttggacag acatatcaag tggatttgaa gcccaatggg
     4441 tcagaaataa tggtcacaaa tgaaaacaaa agggaatata tcgacttagt catccagtgg
     4501 agatttgtga acagggtcca gaagcagatg aacgccttct tggagggatt cacagaacta
     4561 cttcctattg atttgattaa aatttttgat gaaaatgagc tggagttgct catgtgcggc
     4621 ctcggtgatg tggatgtgaa tgactggaga cagcattcta tttacaagaa cggctactgc
     4681 ccaaaccacc ccgtcattca gtggttctgg aaggctgtgc tactcatgga cgccgaaaag
     4741 cgtatccggt tactgcagtt tgtcacaggg acatcgcgag tacctatgaa tggatttgcc
     4801 gaactttatg gttccaatgg tcctcagctg tttacaatag agcaatgggg cagtcctgag
     4861 aaactgccca gagctcacac atgctttaat cgccttgact tacctccata tgaaaccttt
     4921 gaagatttac gagagaaact tctcatggcc gtggaaaatg ctcaaggatt tgaaggggtg
     4981 gattgattaa ttaacggatc cggaattccg cccccccccc ccccccccct ctccctcccc
     5041 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     5101 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     5161 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     5221 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     5281 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     5341 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     5401 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     5461 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     5521 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     5581 ttgaaaaaca cgatgataag cttgccacaa ccatggctga acaagatgga ttgcacgcag
     5641 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     5701 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     5761 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     5821 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     5881 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     5941 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     6001 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     6061 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     6121 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     6181 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     6241 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     6301 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     6361 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggatcggcta
     6421 gagtcgaccc ggacgggacg ctcctgcgcc tgatacagaa cgaattgctt gcaggcatct
     6481 catgagtgtg tcttcccgtt ttccgcctga ggtcactgcg tggatggagc gctggcgcct
     6541 gctgcgcgac ggcgagctgc tcaccaccca ctcgagggct gcagcgctgc agaggccgag
     6601 tgcagaactg ctccaaaggg acctcaaggc tttccgaggg acactaggct gactccatcg
     6661 agccagtgta gagataagct tatcgattag tccaatttgt taaagacagg atatcagtgg
     6721 tccaggctct agttttgact caacaatatc accagctgaa gcctatagag tacgagccat
     6781 agataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct
     6841 gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa aatacataac
     6901 tgagaataga gaagttcaga tcaaggtcag gaacagatgg aacagggtcg accctagaga
     6961 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac
     7021 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa
     7081 agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac
     7141 ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg
     7201 gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt gggggctcgt
     7261 ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg taagctggct
     7321 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     7381 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     7441 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtaga
     7501 tccggctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     7561 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     7621 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     7681 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7741 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     7801 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt actggcttaa
     7861 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     7921 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
     7981 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8041 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8101 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8161 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8221 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8281 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8341 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8401 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8461 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8521 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     8581 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8641 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8701 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8761 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8821 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     8881 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8941 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9001 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9061 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9121 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9181 taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt
     9241 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9301 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9361 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9421 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9481 gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag
     9541 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9601 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9661 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9721 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9781 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9841 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     9901 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca
     9961 agaattagct tggccattgc atacgttgta tccatatcat aatatgtaca tttatattgg
    10021 ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt aatagtaatc
    10081 aattacgggg tcattagttc atagcccata tatggagt
//
