LOCUS       sequence-392696-        8887 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8887)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 8887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2498
FEATURES             Location/Qualifiers
     source          1..8887
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    1825..2413
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1878..1898)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2296..2307)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2485..2718
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2796..2917
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2833..2852)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2887..2906
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2957..3092
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3112..3131)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3113..3131)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3141..3158)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3141..3157)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3150..3172)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3299..3754
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3386..3405)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3596..3617
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3780..3884
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3885..3953,3954..4745)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4103..4122)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4916..5504
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5405..5424
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5658..5675
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5792..5813
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5828..5833,5834..5851,5852..5858)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5866..5882
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5871..5893
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5890..5906
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5890..5906
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5925..5945
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5927..5945
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5973..6199
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6200..6380
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6427..6552
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7045..7278
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7463..7507
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7738..7948
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7760..7956
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7807..7942
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7988..8590
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(7988..8007)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8484..8504
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8633..8750
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgat
     1201 ggagacgcgt aaaccggcgg aacggctggc cttgccatac tcgctgcgca ccgcgcccct
     1261 gggcgttccg gggaccctgc ccggactccc gcggagggac cccctcaggg tcgccctgcg
     1321 tctggacgcc gcgtgctggg agtgggcgcg cagcggctgc gcacggggat ggcagtactt
     1381 gcccgtgccg ctggacagcg ccttcgagcc cgccttcctc cgcaagcgca acgagcgcga
     1441 gcggcagcgg gtgcgctgcg tgaacgaggg ctatgcgcgc ctccgagacc acctgccccg
     1501 ggagctggca gacaagcgcc tcagcaaagt ggagacgctc cgcgctgcca tcgactacat
     1561 caagcacctg caggagctgc tggagcgcca ggcctggggg ctcgagggcg cggccggcgc
     1621 cgtcccccag cgcagggcgg aatgcaacag cgacggggag tccaaggcct cttcggcgcc
     1681 ttcgcccagc agcgagcccg aggagggggg cagctagtaa tgaactagtc cgtaacttga
     1741 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgagttgt acaatgcgtg
     1801 ccgggaagga cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     1861 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     1921 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     1981 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     2041 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     2101 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     2161 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     2221 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     2281 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     2341 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     2401 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     2461 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     2521 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     2581 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     2641 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     2701 tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat
     2761 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     2821 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     2881 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     2941 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     3001 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     3061 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     3121 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     3181 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     3241 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     3301 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     3361 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     3421 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     3481 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     3541 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     3601 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     3661 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     3721 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     3781 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     3841 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     3901 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     3961 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     4021 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     4081 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     4141 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     4201 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     4261 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     4321 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     4381 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     4441 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     4501 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     4561 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     4621 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     4681 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     4741 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     4801 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     4861 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     4921 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     4981 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5041 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     5101 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5161 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5221 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     5281 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     5341 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     5401 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     5461 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     5521 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     5581 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     5641 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     5701 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     5761 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     5821 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     5881 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     5941 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
     6001 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
     6061 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
     6121 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
     6181 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
     6241 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     6301 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     6361 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     6421 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     6481 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     6541 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     6601 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     6661 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     6721 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     6781 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     6841 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     6901 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     6961 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     7021 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     7081 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     7141 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     7201 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     7261 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     7321 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     7381 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     7441 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     7501 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     7561 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     7621 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     7681 catgcattgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     7741 ggcagaagta tgcaaagcat gcgtctcaat tagtcagcaa ccatagtccc gcccctaact
     7801 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     7861 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     7921 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tttctagagg taccaccgga
     7981 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     8041 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     8101 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     8161 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gccgtggcgg tctggaccac
     8221 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     8281 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     8341 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     8401 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     8461 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     8521 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     8581 tgccagttaa cgaattctag atcttgagac aaatggcagt attcatccac aattttaaaa
     8641 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     8701 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     8761 acagggacag cagagatcca ctttggcgcc ggctcgaggc ctgcaggtgc aaagatggat
     8821 aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt
     8881 gggaatt
//
